Analysis of proteins associated with storage root formation in cassava using two-dimensional gel electrophoresis

Cabral, G. B.; Carvalho, Luiz Joaquim Castelo Branco

doi:10.1590/s0103-31312001000100005

Cited by 8 publications

(10 citation statements)

References 14 publications

(4 reference statements)

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“…Unfortunately, detailed characterization of this protein and its function has not since been carried out. Recent comparative proteomic analyses between fibrous and storage roots of cassava further demonstrated protein polymorphism among these two tissues (Cabral and Carvalho 2001;Sheffield et al 2006). Similar to those reported in potato research, leaf physiology and leaf-generated factor(s) are also thought to play important roles in storage root formation in cassava.…”

Section: Introductionsupporting

confidence: 62%

“…Yet, there has not been any report in the literature on molecular and biochemical investigation of leaf genes or proteins involved in the tuberization process of the cassava plant. Particularly, to date, there have been very few papers in the literature report on proteomic analysis in cassava (Cabral and Carvalho 2001;Sheffield et al 2006;Li et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation

Mitprasat

Roytrakul

Jiemsup

et al. 2011

Planta

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Tuberization in cassava (Manihot esculenta Crantz) occurs simultaneously with plant development, suggesting competition of photoassimilate partitioning between the shoot and the root organs. In potato, which is the most widely studied tuber crop, there is ample evidence suggesting that metabolism and regulatory processes in leaf may have an impact on tuber formation. To search for leaf proteins putatively involved in regulating tuber generation and/or development in cassava, comparative proteomic approaches have been applied to monitor differentially expressed leaf proteins during root transition from fibrous to tuberous. Stringent cross comparison and statistical analysis between two groups with different plant ages using Student's t test with 95% significance level revealed a number of protein spots whose abundance were significantly altered (P < 0.05) during week 4 to week 8 of growth. Of these, 39 spots were successfully identified by ion trap LC-MS/MS. The proteins span various functional categories from antioxidant and defense, carbohydrate metabolism, cyanogenesis, energy metabolism, miscellaneous and unknown proteins. Results suggested possible metabolic switches in the leaf that may trigger/regulate storage root initiation and growth. This study provides a basis for further functional characterization of differentially expressed leaf proteins, which can help understand how biochemical processes in cassava leaves may be involved in storage root development.

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Section: Introductionsupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation

Mitprasat

Roytrakul

Jiemsup

et al. 2011

Planta

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“…All soluble proteins as well as peripheral and integral membrane proteins were used for the subsequent mass spectrometric analyses. Protein extract of soluble proteins from Cassava was prepared as previously described [26] from three different root samples and one leaf sample.…”

Section: Preparation Of Plastids and Protein Extracts From Spinach Bmentioning

confidence: 99%

A workflow to increase the detection rate of proteins from unsequenced organisms in high‐throughput proteomics experiments

et al. 2007

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We present and evaluate a strategy for the mass spectrometric identification of proteins from organisms for which no genome sequence information is available that incorporates cross-species information from sequenced organisms. The presented method combines spectrum quality scoring, de novo sequencing and error tolerant BLAST searches and is designed to decrease input data complexity. Spectral quality scoring reduces the number of investigated mass spectra without a loss of information. Stringent quality-based selection and the combination of different de novo sequencing methods substantially increase the catalog of significant peptide alignments. The de novo sequences passing a reliability filter are subsequently submitted to error tolerant BLAST searches and MS-BLAST hits are validated by a sampling technique. With the described workflow, we identified up to 20% more groups of homologous proteins in proteome analyses with organisms whose genome is not sequenced than by state-of-the-art database searches in an Arabidopsis thaliana database. We consider the novel data analysis workflow an excellent screening method to identify those proteins that evade detection in proteomics experiments as a result of database constraints.

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“…A formação de raízes de mandioca a campo é conseqüência, principalmente, da elevada produção de elementos do xilema e fl oema provenientes da atividade do câmbio, seguida do depósito de substâncias de reserva, principalmente amido e sacarose, nas células parenquimáticas (Cabral & Carvalho 2001).…”

Section: Introductionunclassified

“…Kurian 1977;Cabral & Carvalho 2001). No caso da batata, inicialmente ocorre intensa divisão celular, seguida do alongamento das células, divisões das células parenquimáticas próximas ao fl oema e depósito de amido (Ewing & Struik 1992).…”

Section: Introductionunclassified

Microtuberização in vitro de cultivares de mandioca: aspectos morfológicos e anatômicos

et al. 2010

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RESUMO -(Microtuberização in vitro de cultivares de Mandioca: aspectos morfológicos e anatômicos). Objetivou-se induzir o processo de microtuberização in vitro de cultivares de mandioca (Manihot esculenta Crantz.), em dois tipos de meio, sob a ação de diferentes reguladores de crescimento e concentrações de sacarose, e verifi car as alterações estruturais decorrentes do processo de tuberização. Foram analisadas a infl uência do tipo de meio, a ação do BAP e ANA e doses de sacarose (3%, 6% e 8% p/v) através das características comprimento da parte aérea, o número médio de raízes, o padrão morfológico das raízes tuberiformes, bem como características anatômicas. Verifi ca-se que as cultivares de mandioca respondem diferentemente à microtuberização in vitro. A cultivar Parazinha responde melhor à indução de raízes tuberiformes, tendo o tratamento constituído de 0,4 μM BAP, 1,6 μM ANA e 8% sacarose como indutor na formação da raiz tuberosa, tanto em meio semisólido quanto em meio líquido. Para o comprimento da parte aérea, da raiz mais longa e o número de raízes destaca-se a Mantiqueira, porém, em meio de indução à raiz tuberiforme, foi considerada recalcitrante. Anatomicamente, a microtuberização in vitro caracteriza-se pela presença de grãos de amido. Palavras-chave: Manihot esculenta (Crantz), cultura in vitro, regulador de crescimento, tuberização ABSTRACT -(In vitro microtuberization of Cassava cultivars: morphological and anatomical aspects). The goal of this work was to induce in vitro tuberization of cassava cultivars (Manihot esculenta Crantz.) in two kinds of media, under the infl uence of different growth regulators and sucrose concentrations, as well as to determine subsequent structural alterations due to the tuberization. The infl uence of the kind of media, action of BAP and NAA and doses of sucrose (3%, 6% and 8% w/v) were analyzed by measuring the length of the aerial portion, number of roots, morphological pattern of the tuber roots, as well as anatomical changes. There were differences among the cultivars regarding the response to in vitro tuberization. Cultivar Parazinha had better induction of tuber roots, where treatment was with 0.4 μM BAP, 1.6 μM NAA and 8% sucrose as inducing medium, whether in solid or liquid medium. Cultivar Mantiqueira had the tallest aerial portion, longest root and largest number of roots, but the induction of tuber roots was recalcitrant. Anatomically, the microtuberization in vitro was characterized by the presence of starch grains.

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Analysis of proteins associated with storage root formation in cassava using two-dimensional gel electrophoresis

Cited by 8 publications

References 14 publications

Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation

Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation

A workflow to increase the detection rate of proteins from unsequenced organisms in high‐throughput proteomics experiments

Microtuberização in vitro de cultivares de mandioca: aspectos morfológicos e anatômicos

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