Morphological and immunohistochemical analysis of apoptosis in the cerebellum of rats subjected to focal cerebral ischemia with or without alcoholism model

Carvalho, C.; Tirapelli, Daniela Pretti da Cunha; Rodrigues, Alisson Mendes; Neto, Fermino Sanches Lizarte; Novais, Paulo Cézar; Silva, Jairo Pinheiro; Júnior, Carlos Gilberto Carlotti; Colli, Benedicto Oscar; Tirapelli, Luís Fernando

doi:10.1590/s0102-865020160090000009

Cited by 10 publications

(10 citation statements)

References 20 publications

(19 reference statements)

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“…Carvalho et al 29 reported that the histopathological changes were observed to a greater degree in the cerebellum of rats submitted to the same protocol of ischemia performed by the present study. In groups A and IA the expression of CASPASE-3 was greater than the expression of BCL-2 and XIAP, increased with groups A and IA, especially near the transition region between the granular and molecular layers.…”

Section: ■ Discussionsupporting

confidence: 76%

Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism

Schiavoni

Silva²,

Neto³

et al. 2018

Acta Cir. Bras.

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Section: ■ Discussionsupporting

confidence: 76%

Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism

Schiavoni

Silva²,

Neto³

et al. 2018

Acta Cir. Bras.

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“…Among the findings of recent studies of our group, neuronal loss was also higher in the medial striatum region of the animals of groups I and I + A and the protein expression of CASPASE -3 was higher than that of XIAP in groups I and I + A for both proteins. (Carvalho et al, 2016;Schiavoni et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Research Article Morphological, immunohistochemical and miRNA21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model

Tirapelli¹,

Cirino²,

Silva³

et al. 2020

Genet. Mol. Res.

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Cerebral ischemia is one of the main causes of morbidity and mortality as Abusive use of alcohol also causes health problems, along with considerable social and economic repercussions. We examined how these two conditions affect histopathological signs, morphometrics, expression of apoptosisrelated proteins CASPASE 3 and BCL2, and serum gene expression of miRNA-21, in cerebral ischemia associated with a chronic alcoholism model in brain tissue (striatum, lateral and dorsolteral cortex). Fifty adult Wistar rats were divided into five groups: control (C), sham (S), ischemic (I), alcoholic (A) and ischemic/alcoholic ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 19 (4): gmr18624 D.C.P. Tirapelli et al. 2 groups (I+A). Brain samples were processed for histopathological, morphological, and immunohistochemical analyses for the expression of CASPASE 3 and BCL2, and blood was collected for analysis of miRNA-21 gene expression by real-time PCR. Histopathological changes such as neurons with pyknotic nuclei and diffuse neurons with loss of the contour of their cellular membranes and cytoplasmic edema, were observed in groups I and I+A in all three areas. Protein expression of CASPASE 3 was significantly higher than that of BCL2, with higher expression in groups I and I+A for these two proteins. Serum expression of miRNA-21 was low in all groups, and was slightly higher in groups I and I+A, with no significant differences. The histopathological and morphometric alterations, which were observed mainly in the ischemic animals, correlated with the expression of CASPASE 3. The expression of BCL2 was greater where histopathological changes and expression of CASPASE 3 were less evident. The I and I+A groups presented more histopathological changes, with greater neuronal loss and cerebral edema, and greater expression of CASPASE 3, suggesting that ischemia and alcoholism, when associated, can cause considerable injury and damage to brain tissue.

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“…According toCarvalho et al [28] after one week of acclimatization, the rats were anesthetized by halothane inhalation, fixed in a supine position and intubated with an oro-tracheal cannula. A midline incision was made in the neck, after that the incision was extended 1 cm to the right then both common carotid arteries and the right common jugular vein were exposed carefully by blunt dissection.…”

Section: Induction Of Acute Cerebral Ischemiamentioning

confidence: 99%

Efficacy of cerebrolysin on dentate gyrus of hippocampus after experimentally induced acute ischemic stroke in adult albino rats (Histological, immunohistochemical and biochemical study)

Abdel-aziz

Ahmed

Mohammed

et al. 2019

Egyptian Journal of Histology

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Background: Acute ischemic stroke (AIS) is a brain medical disorder characterized by the sudden loss of blood circulation to an area of the brain, resulting in a loss of its neurologic function. Cerebrolysin is a mixture of neuropeptides and free amino acids. Aim of the work:This study aimed to evaluate the role of cerebrolysin in ameliorating the histological,immunohistochemic al and biochemical harm in post ischemic stroke and also to assess its dose dependent effect. Materials and Methods: Thirty adult male rats were divided into 3 equal groups; control, ischemic and post ischemic treated groups. After dissecting hippocampal dentate gyrus prepared sections were stained with hematoxylin and eosin, Erβ and calretinin proteins. Oxidative stress parameters, TNF α, and HSP-70 assay. Also, Gh receptor gene expression and DNA fragmentation test were measured. Immunoperoxidase reactions for GFAPin astrocyte was estimated. Statistical analysis was conducted. Results: Ischemia group showed decrease number of granule cells with small dark stained nuclei, areas of cell loss and numerous spindle shaped cells in the sub-granular zone. A faint positive immunoreaction for ERβ in nuclei of granular cells and negative reaction for calretinin was detected in granule cells. Antioxidant enzymes in the brain tissue as MDA,TNF α and HSP-70 were significantly elevated in ischemic group compared to control and post ischemic treated groups. Gh receptor gene were decreased, while Fragmentation index of DNA was significantly increased. Administration of 2.5 mg/ kg cerebrolysinshowed partial improvement, whereas, 5 mg/kg dose displayed more ameliorative effects. Increased GFAP immune-expression in the cytoplasm of astrocytes in ischemic group compared to control and post ischemic treated groups Conclusion: Cerebrolysin was effective in experimentally induced AIS (Acute ischemic stroke) in a dose-dependent manner as proved by improving the histological structure, immunohistochemical reactions and biochemical parameters of the dentate gyrus of hippocampus in adult male albino rats.

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Morphological and immunohistochemical analysis of apoptosis in the cerebellum of rats subjected to focal cerebral ischemia with or without alcoholism model

Cited by 10 publications

References 20 publications

Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism

Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism

Research Article Morphological, immunohistochemical and miRNA21 expression manifestations related to cellular apoptosis in focal cerebral ischemia in a rat alcoholism model

Efficacy of cerebrolysin on dentate gyrus of hippocampus after experimentally induced acute ischemic stroke in adult albino rats (Histological, immunohistochemical and biochemical study)

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