The effect of the aqueous extract Peumus boldus on the proliferation of hepatocytes and liver function in rats submitted to expanded hepatectomy

Abstract

PURPOSE:To assess the effect of aqueous extract of Peumus Boldus (AEPB) on the liver proliferative response after parcial hepatectomy of 70% (PH) in rodents. METHODS:Twenty Wistar rats were divided in two groups: AEPB100 (whose rats received 100mg/Kg of AEPB, once a day, orally, in 4 days prior to the first surgical procedure) and Vehicle (whose rats were treated similarly with distilled water). Both groups underwent PH. After 24 hours the remaining livers were removed for studying the proliferation of hepatocytes by Ki-67 and 2mL of blood were collected for serological assessment: cholesterol, glucose, triglycerides, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and total, direct and indirect bilirubin. All data were analyzed by Gaussian distribution. Statistically significant differences between mean values were analyzed using T Student's test. Non-Gaussian data were analyzed using Mann-Whitney's test. RESULTS:The liver of all these rats presented positive staining of Ki-67, indicating liver proliferation. Laboratory results showed no significant difference in serum values between the analyzed groups. The analysis of Ki-67 was significantly more positive in AEPB100 group than in Vehicle group. CONCLUSION:Aqueous extract of Peumus Boldus acute administration exerts significant positive effect on liver regeneration after 24h in rats that underwent parcial hepatectomy, while maintaining unchanged hepatic function.

Effect of aqueous extract of the leaves of Baccharis trimera on the proliferation of hepatocytes after partial hepatectomy in rats

“…Quantitative analysis of PCNA-positive cells revealed average IPs significantly higher (p = 0.02) in the group that used carqueja, which demonstrates the stimulatory effect of Baccharis trimera on rat liver regeneration. Similar findings were verified with the use of other plant extracts as Hyptis pectinata 7 , Sida cordifolia 24 , Hyptis fruticos 11 and Peumus boldus 25 . This positive effect on proliferation of hepatocytes may be related with the antioxidant property of flavonoids, since they regulate the activities of antioxidant enzymes, modulate nuclear receptors and act on gene expression 26 .…”

Cleaner corrosion inhibitors using Peumus boldus Molina formulations in oil well acidizing fluids: gravimetric, electrochemical and DFT studies

“…A model based on the main alkaloid present in Peumus boldus extract (boldine) (Figueiredo et al, 2016;Teixeira et al, 2016) was constructed in order to analyze structural, electronic and quantum properties using calculations based on the DFT method. Fig.…”

Hepatoprotective effect of boldine against diethylnitrosamine‐induced hepatocarcinogenesis in wistar rats

“…In this present study, boldine decreased the positive incidence of oncofetal serum protein (AFP) and CEA tumor biomarkers expression by restoring the normal liver function tests in HCC rats. To validate, the aqueous extract of Peumus boldus had liver regeneration activity with no alternations in liver function in rats with 70% hepatectomy after 24 hours …”