Enduring amnesia induced by ICV scopolamine is reversed by sesame oil in male rats

Abstract

. Intellectual, scientific, conception and design of the study; analysis and interpretation of data; manuscript preparation and writing; final approval. ABSTRACT PURPOSE:To evaluated the long-term effect of scopolamine and sesame oil on spatial memory. METHODS:Memory impairment induced by Intracerebroventricular (ICV) injection of scopolamine hydrochloride (10 μg/ rat).Animals were gavaged for 4 weeks with saline, sesame oil (0.5, 1, or 2 mL/kg/day), or 3 weeks with memantine (30 mg/kg/day) in advance to induction of amnesia. Morris water maze (MWM) test was conducted 6 days after microinjection of scopolamine. Then, blood and brain samples were collected and evaluated for the malondialdehyde (MDA) levels, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities, and total antioxidant status (TAS) and ferric reducing ability of plasma (FRAP). RESULTS: Scopolamine significantly decreased traveled distance and time spent in target quadrant in probe test. Pretreatment of ratswith sesame oil (0.5 mg/kg) mitigated scopolamine-induced behavioral alterations. Measurement of MDA, SOD, and GPX in brain tissue, and FRAP and TAS in blood showed little changes in animals which had received scopolamine or sesame oil.CONCLUSIONS: Intracerebroventricular injection of scopolamine has a residual effect on memory after six days. Sesame oil has an improving effect on spatial memory; however this effect is possibly mediated by mechanisms other than antioxidant effect of sesame oil.

Cholinergic Enhancing and Antioxidant Effect of Vigna subterranea (L.) Verdc. (Fabaceae) Landrace Aqueous Extract on Scopolamine-Induced Amnesia in Male Swiss Mice

“…It selectively blocks muscarinic receptors (M1), hinders cholinergic transmission leading to the development of oxidative stress status [42] and subsequent neurodegeneration associated to the deposit of tau protein and β amyloid. [43] Scopolamine also significantly decreases ACh level and increases AChE activity [44] reason why scopolamine mediated memory impairment can be used as a valid model for investigations on molecules that may reverse AD symptoms [45]. In the present study, the potentials of V. subterranea aqueous extract (100, 200 and 400 mg/kg) in inhibiting behavioral and cognitive impairment associated to repeated scopolamine injection was tested in the Y-maze, NOR, T-maze and MWM.…”

Inhibition of Connexin43 Hemichannels Impairs Spatial Short-Term Memory without Affecting Spatial Working Memory

“…Scopolamine was used as a positive control as different studies report spatial memory impairments following i.c.v. injection of scopolamine (Abbasi et al, 2013; Tabari et al, 2016). In line with literature, we observed impairments in cognitive function with scopolamine in both experimental protocols, implicating that the Y maze protocol is sensitive to impairments after i.c.v.…”

“…Following drug infusion, injection cannulas were left in place for 1 min to allow drug diffusion away from the cannula tip. Doses were based on literature (e.g., Stehberg et al, 2012; Abbasi et al, 2013; Tabari et al, 2016). For TAT-Gap19 and TAT-Gap19I130A, mice received 1 nmol/μL or 2.7 μg/μL i.c.v.…”

Ethanol Extract of Oldenlandia diffusa Herba Attenuates Scopolamine-Induced Cognitive Impairments in Mice via Activation of BDNF, P-CREB and Inhibition of Acetylcholinesterase

“…Since scopolamine is known to show rapid anti-depressant effects in humans [ 36 ], and impair short-term memory and learning ability, it has been used for the Alzheimer’s disease animal model [ 37 ]. The present study revealed that pretreatment of ODH for 28 days protected the scopolamine induced amnesia ICR mouse model.…”