Measurement of testicular volume in Wistar rats using a caliper and ultrasonography in experimental surgery

Brêtas, Salomão; Tatsuo, Edson Samesima; Brêtas, Monica Odete Oliveira; Brêtas, Caroline Oliveira

doi:10.1590/s0102-865020160070000008

Cited by 3 publications

(2 citation statements)

References 21 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ipsilateral and contralateral testes were surgically dissected, weighed and measured and then stored in a −80°C freezer for western blot analysis or fixed for histopathological examination. Testicular volume was calculated as length ( L ) × width ( W ) × height ( H ) × 0.52 15 …”

Section: Methodsmentioning

confidence: 99%

Protective effect of remote ischemic postconditioning in rat testes after testicular torsion/detorsion

Liu

Yan

et al. 2022

Andrology

View full text Add to dashboard Cite

Background: Testicular torsion/detorsion can lead to severe testicular damage.The organ-protective effect of remote ischemic postconditioning (RLIPost) against ischemia/reperfusion injury has been characterized; however, it remains unknown whether RLIPost has a testicular protective effect.Objectives: Here, we tested the hypothesis that RLIPost can protect the testes in a rat model of testicular torsion/detorsion in vivo. Materials and methods:Male Sprague-Dawley rats were assigned to sham-operated, control, or remote liver and limb ischemic postconditioning-treated groups. Testicular torsion/detorsion was performed by 3 h of testicular torsion (720 • clockwise unilateral spermatic cord torsion), followed by 3 h of detorsion. For liver and limb ischemic postconditioning, four cycles of 5 min of liver or limb ischemia with 5-min intermittent reperfusion stimuli were conducted at the onset of testicular reperfusion.Results: Liver and limb ischemic postconditioning significantly ameliorated ipsilateral and contralateral testicular swelling responses, preserved morphological integrity and spermatogenesis and inhibited testicular apoptosis. In addition, RLIPost enhanced the phosphorylation of AKT/ERK1/2/GSK-3β/STAT-3 in the ipsilateral testis while suppressing JNK activation in the ipsilateral and contralateral testes. Discussion and conclusion:To the best of our knowledge, this study is the first to demonstrate the involvement of RLIPost in an animal model of testicular torsion/detorsion. We showed that RLIPost protects both ipsilateral and contralateral testes against testicular torsion/detorsion in vivo, via at least in part, the RISK and SAFE-mediated signaling pathways.

show abstract

Section: Methodsmentioning

confidence: 99%

Protective effect of remote ischemic postconditioning in rat testes after testicular torsion/detorsion

Liu

Yan

et al. 2022

Andrology

View full text Add to dashboard Cite

show abstract

“…The animals were anesthetized via intramuscular injection of ketamine hydrochloride and xylazine cocktail (87.5 mg/kg Ketamine-12.5 mg/kg Xylazine) and testes collected and weighted using an analytical balance. Testicular dimensions were measured with an electronic caliper (Sattiyrch Digital Caliper, Goldmoon Industry, Suzhou, Jiangsu, China) and testicular volumes were calculated using the formula for the calculation of a solid ellipsoid triaxial body, as previously described: 4πabc/3 [ 39 , 40 , 41 ]. This formula was used considering π = 3.14; a = half the height; b = half the width; c = half the length.…”

Section: Methodsmentioning

confidence: 99%

Kcnj16 (Kir5.1) Gene Ablation Causes Subfertility and Increases the Prevalence of Morphologically Abnormal Spermatozoa

Poli

Hasan

Belia

et al. 2021

IJMS

View full text Add to dashboard Cite

The ability of spermatozoa to swim towards an oocyte and fertilize it depends on precise K+ permeability changes. Kir5.1 is an inwardly-rectifying potassium (Kir) channel with high sensitivity to intracellular H+ (pHi) and extracellular K+ concentration [K+]o, and hence provides a link between pHi and [K+]o changes and membrane potential. The intrinsic pHi sensitivity of Kir5.1 suggests a possible role for this channel in the pHi-dependent processes that take place during fertilization. However, despite the localization of Kir5.1 in murine spermatozoa, and its increased expression with age and sexual maturity, the role of the channel in sperm morphology, maturity, motility, and fertility is unknown. Here, we confirmed the presence of Kir5.1 in spermatozoa and showed strong expression of Kir4.1 channels in smooth muscle and epithelial cells lining the epididymal ducts. In contrast, Kir4.2 expression was not detected in testes. To examine the possible role of Kir5.1 in sperm physiology, we bred mice with a deletion of the Kcnj16 (Kir5.1) gene and observed that 20% of Kir5.1 knock-out male mice were infertile. Furthermore, 50% of knock-out mice older than 3 months were unable to breed. By contrast, 100% of wild-type (WT) mice were fertile. The genetic inactivation of Kcnj16 also resulted in smaller testes and a greater percentage of sperm with folded flagellum compared to WT littermates. Nevertheless, the abnormal sperm from mutant animals displayed increased progressive motility. Thus, ablation of the Kcnj16 gene identifies Kir5.1 channel as an important element contributing to testis development, sperm flagellar morphology, motility, and fertility. These findings are potentially relevant to the understanding of the complex pHi- and [K+]o-dependent interplay between different sperm ion channels, and provide insight into their role in fertilization and infertility.

show abstract

Advances in the crosstalk between maternal separation and voluntary ethanol consumption and effects on reproduction

et al. 2022

View full text Add to dashboard Cite

Measurement of testicular volume in Wistar rats using a caliper and ultrasonography in experimental surgery

Cited by 3 publications

References 21 publications

Protective effect of remote ischemic postconditioning in rat testes after testicular torsion/detorsion

Protective effect of remote ischemic postconditioning in rat testes after testicular torsion/detorsion

Kcnj16 (Kir5.1) Gene Ablation Causes Subfertility and Increases the Prevalence of Morphologically Abnormal Spermatozoa

Advances in the crosstalk between maternal separation and voluntary ethanol consumption and effects on reproduction

Contact Info

Product

Resources

About