The acute effects of preoperative ozone theraphy on surgical wound healing

Abstract

PURPOSE:To investigate the effects of preoperative rectal ozone insufflation on surgical wound healing over the proinflammatory cytokines and histopathological changes. METHODS:Twenty one rabbits were divided into 3 groups. Sham, surgical wound, and ozone applied (6 sessions, every other day 70 µg/mL in 12 mL O2-O3 mixture rectally) surgical wound groups were created. TNF-alpha and IL-6 levels from all rabbits were studied at the basal, 24th hour, and 72nd hour. The histopathological examination was done by removing the surgical scar tissue at the end of 72nd hour. RESULTS:TNF-alfa and IL-6 levels were significantly lower compared to the control group, in the rabbits treated with ozone.The increase in angiogenesis, the decrease in the number of inflammatory cells, epidermal and dermal regeneration, better collagen deposition, and increased keratinisation in stratum corneum were observed in the histopathological examination. It was determined that the wound healing noticeably accelerated in the ozone group. CONCLUSION: Preoperative rectal ozone insufflation had a positive effect on surgical wound healing in acute period.

Endometrial angiogenesis induced by uterine insufflation with an oxygen–ozone gas mixture in mares

“…Morphometry, but not morphology, of the endometrial glands was positively affected by intrauterine infusion of the O 2 –O 3 gas mixture containing 42 μg O 3 ml ‐1 . Increased angiogenesis, epidermal regeneration and better collagen deposition have been observed in surgical wounds subjected to O 3 therapy (Sahin et al., 2016). Similarly, the greater area and height of the endometrial glands from the O 3 group suggest a potential beneficial effect of O 3 gas on endometrial health.…”

“…However, in humans, O 3 therapy improves general metabolism (Borrelli & Bocci, 2002) and blood supply to ischaemic tissues (Tylicki et al., 2003). Improved angiogenesis (KrKl et al., 2016), intense collagen synthesis (Sahin et al., 2016) and greater expression of VEGF‐A (Valacchi et al., 2011) are recurrent phenomena found in scar tissues subjected to O 3 therapy.…”

The effects of ozone therapy on wound healing after gingivectomy operations: a controlled clinical study

“…Ozonun iyileşme üzerine olan etkilerinin kısmen enflamatuar sitokinlerdeki azalmadan kaynaklandığı Şahin ve ark. Tarafından gösterilmiştir 28 . Ozon, aynı zamanda in vitro olarak keratinositlerin proliferasyonunu ve VEGF-aracılı neovaskülarizasyonu da artırmaktadır 29,30 .…”

Investigating Inflammatory Markers in Wound Healing: Understanding Implications and Identifying Artifacts

“…For instance, claiming that a treatment can effectively heal a wound by reducing the release of pro-inflammatory factors at specific times raises serious questions because effective wound healing requires the early release of pro-inflammatory markers. Furthermore, the crucial role of the pro-inflammatory cytokines (such as IL-8 and IL-6) in the late stages of wound healing, such as re-epithelization and remodeling, cannot be underestimated; thus, significantly modulating the release of the pro-inflammatory markers is not always in favor of enhanced wound healing, and the possible impact of these materials on releasing anti-inflammatory factors should be considered. − Likewise, the modulatory effects of materials on the release of anti-inflammatory markers do not sufficiently illustrate their potential for wound healing; in order to substantiate the therapeutic efficacy, such evaluations should be supplemented by examining their impact on a wide range of other pro- and anti-inflammatory markers.…”