Effect of Otostegia persica extract on ischemia/reperfusion induced renal damage in diabetic rats. A biochemical study

Abstract

PURPOSE:To evaluate the effect of Otostegia persica (O. persica) extract on renal damage induced by ischemia/reperfusion (I/R) in diabetic rats. METHODS:Forty-eight rats were subjected to right nephrectomy; then, they were allocated into six groups: Sham; Diabetic sham; I/R; Diabetic I/R; I/R+O. persica; Diabetic I/R+O. persica. Diabetes was induced by streptozotocin (200 mg/kg, i.p.). O. persica (300 mg/ kg/day, p.o) was administered for 2 weeks. On the 15th day, ischemia was induced in left kidney for 60 min, followed by reperfusion for 24h. Renal functional and biochemical markers were estimated.RESULTS: I/R in both normal and diabetic rats, induced a significant elevation in serum levels of urea and creatinine (p<0.05). Renal I/R induced a significant increase of malondialdehyde, myeloperoxidase and nitric oxide concentrations associated with significant reduction in superoxide dismutase and catalase activities in comparison with the sham group (p<0.05). Diabetic rats that underwent renal I/R exhibited a significant increase in all the studied parameters with a reduction in the antioxidant enzymes as compared to nondiabetic rats (p<0.05). These deleterious effects associated with renal I/R were improved by the treatment with O. persica (p<0.05). CONCLUSION:Otostegia persica pretreatment protected the renal injury from ischemia-reperfusion in diabetic rats.

A Review of the Phytochemistry, Traditional Uses, and Biological Activities of the Genus Ballota and Otostegia

“…Additionally, a reduction in lipidic oxidation was proved by the decrease in MDA values and the increase in GSH values. The ethanolic extract of O. persica was demonstrated to possess protective effects by preventing renal damage induced by ischemia/reperfusion induced in diabetic rats [289]. The hematic renal function indicators were ameliorated after treatment with 300 mg/kg b. w. of the extract for 2 wk: urea from 67.6 to 36.1, creatinine from 2.32 to 1.32, glucose from 378.6 to 147.2.…”

The renoprotective effect of oral Tadalafil pretreatment on ischemia/reperfusion injury in rats

“…I/R has been studied in several experimental models, and some synthetic or plant extracts have been used, supposedly to protect the ischemic and reperfused organs [12][13][14][15] . Inhibitors of type 5 phosphodiesterase, such as sildenafil, which have well-characterized effects in the arterial flow, increase the production of cyclic guanosine 3,5-monophosphate (cGMP), consequently of nitric oxide, resulting in vasodilation 16 .…”

“…Various drugs have been used in experimental studies, supposed to protect the organs from the effects of I/R [12][13][14][15] . Some the attention has turned to phosphodiesterase type-5 inhibitors such as sildenafil, which increase the concentration of cyclic guanosine 3,5-monophosphate (cGMP) resulting in release of nitric oxide, and subsequent arterial vasodilation 16 .…”