Effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitro

Zhang, Wei; Wang, Yan; Sui, Xianxian; Sun, Yulin; Chen, Daohai

doi:10.1590/s0102-865020160020000006

Cited by 10 publications

(9 citation statements)

References 19 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, therapeutic effect of low molecular weight chitosan containing sepia ink has been demonstrated in ethanol-induced gastric ulcer in rats [22]. Zhang et al showed the beneficial effect of chitin and sepia ink hybrid sponge on the healing of burning wound in rat model via promoting the phagocytosis ability of macrophages [23]. Moreover, sepia ink extract provided a curative effect and anti-oxidative capacity on bile duct ligation, and could ameliorate the complications of live cholestasis [24].…”

Section: Discussionmentioning

confidence: 99%

Sepia Ink Oligopeptide Induces Apoptosis of Lung Cancer Cells via Mitochondrial Pathway

Wang

Chen

Zhou

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Our previous study suggested the anti-tumor activity of sepia ink oligopeptide (SIO). Here we sought to investigate the underlying molecular mechanism. Methods: Cell proliferation was evaluated by cell counting kit-8 (CCK-8) assay. Cell apoptosis was determined by Annexin V/Propidium Iodide (PI) staining. The mitochondria pathway was characterized by quantification of Bcl-2, Bax, Caspase-9 and Cyto-C. The death receptor pathway was analyzed by determinement of Fas, Caspase-8 and NIK. The endoplasmic reticulum (ER)-dependent pathway was determined by measurement the expression of CHOP, Caspase-12, GRP78 and Calpain. The associated gene expression was quantified by RT-PCR and protein level was determined by immunoblotting. Results: We demonstrated treatment with structurally modified SIO (CSIO, 5 µM) significantly inhibited cell proliferation and induced apoptosis in lung cancer cell line A549. The mitochondrial pathway, death receptor pathway and ER stress induced apoptosis were stimulated upon CSIO treatment. The administration with respective inhibitors including midiv-1 (50 µM for 2 h), PDTC (20 µM PDTC for 30 min) and ALLN (20 mM ALLN for 5 h) readily reversed the apoptosis inducing effect of CSIO. Conclusion: Our data demonstrates that CSIO is capable of induction apoptosis in lung cancer cell line, which is mediated by all three classical apoptotic pathways. Our results warrant further in vivo investigations of the anti-tumor potential of CSIO.

show abstract

Section: Discussionmentioning

confidence: 99%

Sepia Ink Oligopeptide Induces Apoptosis of Lung Cancer Cells via Mitochondrial Pathway

Wang

Chen

Zhou

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…As shown in Figure 8, the wound healing rates of the CCD, control, A, B, C, and D groups, respectively, were 31…”

Section: Effect On Infectious Wound Healingmentioning

confidence: 88%

“…For example, they are all safe and nontoxic, enabling use in the food industry as a food preservative and additive. 31,32 It is also considered that natural materials have low toxicity and are especially suitable for modified wound dressings. Because of the destruction of the defense barrier of burn patients both internally and on the body surface, patients' immune ability typically decreases significantly, and extensive tissue necrosis and the invasion of bacteria can lead to wound infection.…”

Section: Discussionmentioning

confidence: 99%

Preparation of chitin-amphipathic anion/quaternary ammonium salt ecofriendly dressing and its effect on wound healing in mice

Zhou¹,

Yang²,

Yang³

et al. 2018

IJN

View full text Add to dashboard Cite

ObjectiveThis study aimed to prepare an eco-friendly dressing using a chitin-derived membrane with amphipathic anion/quaternary ammonium salt designed for antibacterial purposes.MethodsFour dressings were prepared and group A was chitin, group B was chitin + amphiphilic ion, group C was chitin + quaternary ammonium salt, group D was chitin + amphiphilic ion + quaternary ammonium salt.ResultsIn the group D material, precipitation of adherent composite ions was observed. The contact angle test showed that the material was hydrophilic. The drug loading rate in groups B, C, and D was 40–50 (ug:mg), the entrapment efficiency was 70%–75% (P>0.05), and the cumulative release percentages were 87.3%, 88.7%, and 90.2% after 72h for group B, C, and D, respectively. The anti-bacterial activity in vitro was in the order D>C>B>A> control (P>0.05). The anti-pollution activity in vitro was in the order D>B>C>A (P<0.05). The cell proliferation inhibition test showed slight proliferation inhibition (P<0.05) only on the seventh day for group D. Seven days after injury, the wound healing rate was in the order D>C> commercial chitin dressing >B>A> control (P<0.05), and the length of the neonatal epithelium also showed the same trend. Additionally, PCNA and CD31 expression indicated that cell proliferation and angiogenesis were enhanced when skin defects were covered with the D group material (P<0.05).Conclusionchitin-amphiphilic ion/quaternary ammonium salt dressing was successfully prepared. The antibacterial and antipollution effects of the prepared material (group D) were both very good, acting to promote wound healing.

show abstract

“…Chitin patch caused more abundant of new blood vessels with a lower inflammation score and reduced thickness of fibrous capsule compared with the PP mesh at each time point ( p < 0.01). The anti‐inflammatory and healing‐promoting effects of the chitin materials has been linked to the influences on secretion of kinds of cytokines and activating macrophages, including inhibitory effects of IL‐6 and TNF‐α and promoting effect of FGF1 and TGF‐β1 . Therefore, regulatory effects of the chitin patch on related cytokines deserved our concern.…”

Section: Resultsmentioning

confidence: 99%

“…The anti-inflammatory and healing-promoting effects of the chitin materials has been linked to the influences on secretion of kinds of cytokines and activating macrophages, including inhibitory effects of IL-6 and TNF-a and promoting effect of FGF1 and TGF-b1. [27][28][29] Therefore, regulatory effects of the chitin patch on related cytokines deserved our concern.…”

Section: Morphological Evaluation Of the Repaired Sitementioning

confidence: 99%

Biomechanical properties and healing effects of chitin patch in a rat full‐thickness abdominal wall defect model

et al. 2017

View full text Add to dashboard Cite

Hernia repair is usually accompanied with the implantation of a synthetic mesh, which frequently results in a foreign body response and serious complications. In the present study, a novel biodegradable chitin-based hernia patch was prepared and characterized. Biomechanical properties and biodegradability of the chitin patch were quantified in vitro and in vivo. In repair of the rat abdominal wall full-thickness defect model, the chitin patch induced more abundant new blood vessels with milder tissue inflammation and fibrosis compared with polypropylene mesh. Chitin patch effectively inhibited excessive secretion of inflammation-associated cytokines (IL-6 and TNF-α) (p < 0.01) and significantly increased the secretion of healing-related cytokines (FGF1 and TGF-β1) (p < 0.01). Accompanied by biodegradation of the chitin patch, intra-abdominal adhesions caused by the chitin patch decreased significantly, and the tensile strength of the repaired site could meet the biomechanical requirements of human abdominal wall. After the one-year observation period, the defected abdominal wall returned to the appropriate thickness with no obvious complication or hernia occurrence. In a conclusion, the newly designed chitin patch showed good biomechanical properties and satisfactory healing effects on the full-thickness defect of abdominal wall, which makes it promising candidate for clinical hernia treatment. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1349-1357, 2018.

show abstract

Effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitro

Cited by 10 publications

References 19 publications

Sepia Ink Oligopeptide Induces Apoptosis of Lung Cancer Cells via Mitochondrial Pathway

Sepia Ink Oligopeptide Induces Apoptosis of Lung Cancer Cells via Mitochondrial Pathway

Preparation of chitin-amphipathic anion/quaternary ammonium salt ecofriendly dressing and its effect on wound healing in mice

Biomechanical properties and healing effects of chitin patch in a rat full‐thickness abdominal wall defect model

Contact Info

Product

Resources

About