Rabbit olfactory stem cells. Isolation protocol and characterization

Ercolin, Anna Carolina Mazeto; Roballo, Kelly Cristine Santos; Casals, Juliana Barbosa; Pieri, Naira Caroline Godoy; Souza, Aline Fernanda de; Barreto, Rodrigo da Silva Nunes; Bressan, Fabiana Fernandes; Feitosa, Matheus Levi Tajra; Miglino, María Angélica; Meirelles, Flávio Vieira; Ambrósio, Carlos Eduardo

doi:10.1590/s0102-865020160010000009

Cited by 16 publications

(25 citation statements)

References 27 publications

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“…Located in the olfactory region of the nasal cavity, OM-MSCs are primarily derived from neural crest cells, have a high proliferation rate, self-renewal ability and multiple lineage diferentiation capabilities [83]. These cells have already been isolated from human and mice [84], rat [85], rabbit [86] and dog [87], both with in vivo [84] and post-mortem protocols [86].…”

Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

Alvites¹,

Santos²,

ProençaVarejão³

et al. 2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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The peripheral nerve injury after trauma is a common occurrence in both human and veterinary medicine and has severe consequences for the survival and quality of life of the patients. Despite the continuous eforts and the creation of diverse medical and surgical techniques, the harmful efects of this type of injury are far from being overcome. Regenerative medicine has been growing in the scientiic milieu as a new therapeutic approach for diferent situations. Among the cell-based therapies explored, the mesenchymal stem cells are evidenced by their features, versatility and potential applications. The olfactory mucosa mesenchymal stem cells, components of the olfactory system and identiied in the lamina propria, were newly identiied and are still undergoing characterization, appearing as a new promise in the regenerative therapy of several tissues but with special emphasis on the nervous system in general and the peripheral nervous system in particular, for which they appear to have special regenerative aptitude.

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Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

Alvites¹,

Santos²,

ProençaVarejão³

et al. 2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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“…Esse método deve seguir as normas bioéticas vigentes para o procedimento de eutanásia. Na coleta realizada após a decapitação, o maxilar inferior e os músculos faciais devem ser removidos a partir da parte anterior dos incisivos, também se deve remover o osso etmoide que cobre a cavidade nasal , Ercolin et al 2016). Localizadas na parte medial do nariz com coloração laranja/marrom, as conchas nasais ficarão visíveis e também devem ser descartadas.…”

Section: Principais Métodos De Coleta Das Células-tronco Do Epitélio unclassified

“…No septo, a mucosa olfatória deve ser cortada ao longo de três locais: no arco da lâmina horizontal, na lâmina cribriforme e no teto da cavidade nasal. O procedimento de coleta em ambos os lados da cavidade nasal não deve ser superior a 10 minutos e o material coletado deve ser transferido para placas contendo meio de cultivo específico para a remoção do muco , Ercolin et al 2016. Para que não se faça necessário à eutanásia e posteriormente o descarte dos ratos doadores, Stamegna et al (2014) desenvolveu um método que permite a coleta em animais vivos .…”

Section: Principais Métodos De Coleta Das Células-tronco Do Epitélio unclassified

“…A diferenciação celular pode ser realizada utilizando meios de cultivo que estimulam a diferenciação osteogêni-ca, adipogênica ou neurogênica (Ercolin et al 2016). Alves (2009) utilizou nas células-tronco provenientes de cães com 60 dias de vida intra-uterina, 2-fosfato ácido ascórbico e β-Glicerolfosfato para diferenciação osteogênica, para a diferenciação adipogênica utilizou-se meio de cultivo com dexametasona, indometacina, IBMX (3-isobutil-1-metilxantina), insulina, e na diferenciação neurogênica apenas monoetilglicerol (3-mercapto-1,2-propanediol).…”

Section: Diferenciação Das Células-tronco Do Epitélio Olfatório Para unclassified

“…Alves (2009) utilizou nas células-tronco provenientes de cães com 60 dias de vida intra-uterina, 2-fosfato ácido ascórbico e β-Glicerolfosfato para diferenciação osteogênica, para a diferenciação adipogênica utilizou-se meio de cultivo com dexametasona, indometacina, IBMX (3-isobutil-1-metilxantina), insulina, e na diferenciação neurogênica apenas monoetilglicerol (3-mercapto-1,2-propanediol). Em outro estudo realizado por Ercolin et al (2016) com coelhos, utilizou-se também monoetilglicerol na diferenciação neuronal e meios de diferenciação comercializados na diferenciação osteogênica e adipogênica.…”

Section: Diferenciação Das Células-tronco Do Epitélio Olfatório Para unclassified

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Células-tronco derivadas do epitélio olfatório: perspectivas terapêuticas na medicina veterinária

et al. 2016

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ABSTRACT.-Mazzarella R., Garnica T.K., Roballo K. The olfactory epithelium (OE) is a promising source of stem cells (OESC) for therapeutic use in veterinary and human medicine, especially in diseases correlated with the peripheral (spinal cord) and central (brain and brainstem) nervous system (CNS), because of its ability to differentiate into neurons, astrocytes and oligodendrocytes cells. In humans, OESC has been used primarily in therapeutic trials for degenerative diseases such as Alzheimer and Parkinson. In animals, the frequency of corresponding cases of chronic or acute neurodegenerative diseases is very low, because of the difficulty of a definitive diagnosis; thus, the focus of cell therapy research are mostly mechanical spinal cord injuries. Due to the lack of normalization and selection of the best methodologies for comparative studies, this review aims to analyze recent reports on the potential use of stem cells from the olfactory epithelium in cell therapies and to discuss the main challenges and future prospects in veterinary medicine.INDEX TERMS: Olfactory epithelium, stem cell, spinal cord therapies, veterinary medicine.¹ Recebido em 5 de fevereiro de 2016.Aceito para publicação em 3 de maio de 2016.

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The Nasal Cavity of the Rat and Mouse—Source of Mesenchymal Stem Cells for Treatment of Peripheral Nerve Injury

Alvites

Caseiro

Pedrosa

et al. 2018

The Anatomical Record

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The nasal cavity performs several crucial functions in mammals, including rodents, being involved in respiration, behavior, reproduction, and olfaction. Its anatomical structure is complex and divided into several regions, including the olfactory recess where the olfactory mucosa (OM) is located and where the capture and interaction with the environmental odorants occurs. Among the cells of this region are the OM mesenchymal stem cells (MSCs), whose location raises the possibility that these cells could be involved in the peculiar ability of the olfactory nerve to regenerate continuously throughout life, although this relationship has not yet been confirmed. These cells, like all MSCs, present functional characteristics that make them candidates in new therapies associated with regenerative medicine, namely to promote the regeneration of the peripheral nerve after injury. The availability of stem cells to be therapeutically applied essentially depends on their collection in the tissue of origin. In the case of mice and rat's OM-MSCs, knowledge about the anatomy and histology of their nasal cavity is essential in establishing effective collection protocols. The present article describes the morphological characteristics of rodent's OM and establishes an alternative protocol for access to the olfactory recess and collection of the OM. Anat Rec, 301:1678-1689, 2018. © 2018 Wiley Periodicals, Inc.

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Rabbit olfactory stem cells. Isolation protocol and characterization

Cited by 16 publications

References 27 publications

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

Células-tronco derivadas do epitélio olfatório: perspectivas terapêuticas na medicina veterinária

The Nasal Cavity of the Rat and Mouse—Source of Mesenchymal Stem Cells for Treatment of Peripheral Nerve Injury

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