Effect of low-intensity therapeutic ultrasound on wound healing in rats subjected to third-degree burns

Mendonça²,

et al. 2016

Acta Cir. Bras.

PURPOSE:To evaluate the effects of low intensity ultrasound on the healing process of third degree burn wounds in experimentally induced diabetic Wistar rats. METHODS:One hundred rats were divided into: control group; non-diabetic treated group; diabetic control group; diabetic treated group. The therapy was performed with a 3MHz ultrasound application, pulsed emission at 100Hz frequency, modulated at 20% with a dosage of 0.5W/cm 2 during three minutes throughout 30 days. The surgical debridement of the wound was performed once at day 2. The wounds were morphometrically, macroscopically and microscopically evaluated at 3, 7, 14, 21 and 30 days. RESULTS:The wound contraction and collagen quantification were higher in all treated groups. Macroscopically, necrosis was higher in the diabetic control group. Granulation tissue was higher in treated groups during the proliferative and remodeling phase.Microscopically, there were greater mononuclear inflammatory infiltration, angiogenesis and fibroblast quantification in treated groups during the proliferative and remodeling phases.CONCLUSIONS: therapeutic ultrasound is beneficial in the inflammatory and proliferative phases of the healing process because it controlled the necrotic tissue, increased the granulation tissue and wound contraction. However in the remodeling phase it is not beneficial because of the continued angiogenesis and a mononuclear inflammatory infiltration.

mentioning

confidence: 72%

Low intensity ultrasound therapy induces angiogenesis and persistent inflammation in the chronic phase of the healing process of third degree burn wounds experimentally induced in diabetic and non-diabetic rats

Mendonça²,

et al. 2016

Acta Cir. Bras.

“…Walker and Mason 6 used 100°C as water temperature and 4 studies 18 , 19 , 22 , 25 did the same. More recent studies 16 , 17 , 23 used 90°C to 98°C as water temperatures, yet, the results were the same as those using 100°C; 2 studies [ 17 , 21 ] used 70°C to 78°C so as to obtain a more superficial injury; and 3 studies 14 , 15 , 17 used 80°C a 89°C and observed a median injury.…”

Section: Discussionmentioning

confidence: 99%

“…used 7 to 9 seconds to obtain a median injury (second degree); however, Walker and Mason 6 observed the same result using 4 seconds. In order to observe a full thickness burn (third degree), 7 studies used the same amount of seconds as Walker and Mason, i.e., 10 seconds 14 , 16 , 17 , 19 , 22 , 23 , 25 . Vana et al 20 .…”

Section: Discussionmentioning

confidence: 99%

Experimental models of scald burns. A scope review

et al. 2019

Purpose:To conduct a scope review of the experimental model described by Walker and Mason, by identifying and analyzing the details of the method.Methods:The authors searched Pubmed-Medline, Cochrane-Bireme and PEDro databases for articles published between January 2016 and December 2018, using the following search queries: burns, burn injuries, models animal, and animal experimentation. All articles whose authors used Walker and Mason's model - with or without changes to the method in Wistar rats - were included in this study.Results:The search identified 45 mentions of Walker and Mason's model; however, after reading each summary, 20 were excluded (of which 5 due to duplicity). The inconsistencies observed after the scope review were: water temperature, length of time of exposure of the experimental model's skin to water, extent of the burnt area, and the description of the thickness/depth of the injury.Conclusions:Reproducibility of a scientific method is the basis to prove the veracity of the observed results. Thus, it is necessary to have a greater number of publications that adopt a reproducible scientific method, for this review found inconsistencies in the description of Walker and Mason's model.

“…The observations included the epidermis, dermis, blood vessels, cells and organelles. Another two evaluators used the histological changes in burn wound healing scoring system [19] to perform blind histological evaluations.…”

Section: The Test Of Hande Stainingmentioning

confidence: 99%

Burn Ointment Promoted Cutaneous Wound for Burns and Scalds by Modulating the PI3K/AKT/mTOR Signaling Pathway

Gan

Yao

et al. 2020

Preprint

Background: Burn injury is common, burn ointment (BO) is a common preparation used to treat burns and scalds in folk as an effective remedy for burn healing. The present study was undertaken to evaluate the healing effect and related underlying mechanisms of BO in a model of deep second-degree thermal burn by animal experiments. Methods: BO is was made up of a combination of extracts from several traditional Chinese medicine and borneol, solid paraffin, rapeseed oil, and and its quality control was assessed. The acute toxicity test and skin irritation test were evaluated by rats. The anti-inflammatory effect was revealed by using inflammation animal models, including the xylene-induced auricle swelling in mice and carrageenan-induced toe swelling in rats. The hot plate test was used to evaluate its analgesic activity. Moreover, the experiments of knife and a deep second-degree burn wound were used to explore the effect of BO in promoting wound healing. On days 7, 14 and 28, the wounds were digitally photographed by a camera and after sacrifice of the SD rats, skin samples were obtained for performing H&E staining, immunohistochemical staining and Western Blotting examination. In addition, The expressed of TNF-α, TGF-β1 and VEGF in serum were detected by ELISA kits. Results: BO had no toxicity or side effects on the skin and liver or kidney function. BO could significantly inhibit auricular swelling in mice, paw welling in rats and markedly prolonged the latencies of licking paws in mice; it also could accelerate the process of wound healing and repair scar by promoting the formation of new epithelial tissue. In addition, BO significantly reduced the content of TNF-α and markedly increased the content of VEGF and TGF-β1 in the serum. Moreover, BO promoted the expression of collagen I. Furthermore, it increased the ratio of p-PI3K/PI3K, p-AKT/AKT and p-mTOR/mTOR in the PI3K / AKT / mTOR pathway.Conclusions: BO could effectively reduce inflammation and pain, and effectively accelerate the healing process of deep second-degree burn wounds. And the mechanism of BO promoting wound healing may be related to activate PI3K / AKT / mTOR pathway. therefore, it may be recommended as a promising topical medication for treating burn wounds in the future clinical trials.