Remote limb ischemic post-conditioning attenuates ischemia-reperfusion injury in rat skin flapby limiting oxidative stress

Zhang, Yi; Xu, Hua; Wang, Tao; He, Jinguang; Wei, Jiao; Wang, Tingliang; Dong, Jie

doi:10.1590/s0102-865020160010000003

Cited by 11 publications

(6 citation statements)

References 23 publications

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“…Their group used mice to conduct three cycles of 5-min ischemia and subsequent 5-min reperfusion of bilateral femoral arteries to show that LRIP significantly improved neurological outcomes likely by reducing oxidative stress and initiating the Nrf2-ARE pathway. Zhang et al, Zhou et al, and Kadkhodaee et al all investigated the effect of LRIP against I/R injury in rats; all groups showed a significant decrease in the level of MDA after LRIP ( 64 , 105 , 106 ). We performed studies in rats to understand the role of nitrotyrosine, mRNA of P22 phox , and xanthine oxidase and how they contribute to oxidative damage.…”

Section: Mechanisms Of Limb Remote Ischemic Pre- Per- and Postcondimentioning

confidence: 99%

Limb Remote Ischemic Conditioning: Mechanisms, Anesthetics, and the Potential for Expanding Therapeutic Options

et al. 2018

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Novel and innovative approaches are essential in developing new treatments and improving clinical outcomes in patients with ischemic stroke. Remote ischemic conditioning (RIC) is a series of mechanical interruptions in blood flow of a distal organ, following end organ reperfusion, shown to significantly reduce infarct size through inhibition of oxidation and inflammation. Ischemia/reperfusion (I/R) is what ultimately leads to the irreversible brain damage and clinical picture seen in stroke patients. There have been several reports and reviews about the potential of RIC in acute ischemic stroke; however, the focus here is a comprehensive look at the differences in the three types of RIC (remote pre-, per-, and postconditioning). There are some limited uses of preconditioning in acute ischemic stroke due to the unpredictability of the ischemic event; however, it does provide the identification of biomarkers for clinical studies. Remote limb per- and postconditioning offer a more promising treatment during patient care as they can be harnessed during or after the initial ischemic insult. Though further research is needed, it is imperative to discuss the importance of preclinical data in understanding the methods and mechanisms involved in RIC. This understanding will facilitate translation to a clinically feasible paradigm for use in the hospital setting.

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Section: Mechanisms Of Limb Remote Ischemic Pre- Per- and Postcondimentioning

confidence: 99%

Limb Remote Ischemic Conditioning: Mechanisms, Anesthetics, and the Potential for Expanding Therapeutic Options

et al. 2018

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“…The cessation of oxygen and nutrition supply caused by the ischemia leads to cell damage. During reperfusion, besides the cellular hypoxic damage, inflammatory reactions and free radical release lead to further tissue damage, resulting in hemorheological disturbances among others [5,8,31,33]. The micro-rheological differences can lead to further microcirculatory disturbances that cause decreased tissue perfusion again [5,18,28,30] (Fig.…”

Section: Discussionmentioning

confidence: 99%

Impact of groin flap ischemia-reperfusion on red blood cell micro-rheological parameters in a follow-up study on rats

Magyar

Molnár

Nachmias

et al. 2021

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BACKGROUND: Flap hypoperfusion or ischemia-reperfusion (I/R) may occur during preparation-transposition procedures and by postoperative thrombotic complications. Behind the microcirculatory disturbances micro-rheological alterations are also supposed. OBJECTIVE:We aimed to investigate the groin flap I/R with following-up micro-rheological parameters.METHODS: Anesthetized rats were subjected to Control or I/R groups. Groin flaps were prepared bilaterally, pedicled on the superficial epigastric vessels. In Control group the flaps were re-sutured after one hour, while in I/R group microvascular clips were applied on the pedicles for 60 minutes, then the flaps were repositioned. Besides daily wound control, before the operation and on the 1st, 3rd, 5th, 7th and 14th postoperative days blood samples were collected for testing red blood cell (RBC) deformability (rotational ektacytometry) and aggregation (light-transmission aggregometry). RESULTS:RBC deformability significantly worsened by the 3rd-7th postoperative day in I/R group. RBC aggregation enhanced significantly by the 1st day, in I/R group it remained elevated on the 3rd day as well. In a complicated case with unilateral flap necrosis, RBC deformability and aggregation worsening was outlined from its group (base, 1st, 3rd day). CONCLUSION:Wound healing affected micro-rheological parameters in the early postoperative period. Flap I/R exacerbated the alterations. The parameters markedly worsened in case of flap necrosis.

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“…The perforator flap, which is an essential component, tends to contribute to flap transfer and/or tissue reconstruction (Scaglioni et al, 2018; Stekelenburg et al, 2017). Several procedures, such as IPC, have been proven to confer protection against flap I/R injury, not only by preclamping the flap pedicle but also by blocking the limb blood flow (RIPC) (Küntscher et al, 2002, 2005; Moon et al, 2008; Zhang et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

ZNF667 attenuates leukocyte‐endothelial adhesion via downregulation of P‐selectin in skin flap following remote limb ischemic preconditioning

Chen

Liu²,

et al. 2021

Cell Biology International

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We assessed the effects and potential mechanism of romote ischemic preconditioning (RIPC) on leukocytes‐endothelium cell adhesion in the flap microvessel after ischemia‐reperfusion (I/R) injury. Eight hours after reperfusion, edema and intravascular leukocyte aggregation were reduced and microvessels were more obvious in the group with superficial inferior epigastric artery (SIEA) perforator flap (SIEA‐flap) subjected to RIPC than in the I/R group. Zinc finger protein 667 (ZNF667) was significantly increased but P‐selectin was decreased in the flaps subjected to RIPC, compared to those in the I/R group. The low expression of P‐selectin was associated with ZNF667 expression and activation in human dermal microvascular endothelial cells in response to hypoxic preconditioning. ZNF667 bound to the P‐selectin promoter region, suppressing its transcription through a special core sequence. The ablation of P‐selectin by small interfering RNA effectively prevented the leukocytes‐endothelium cell adhesion effect of ZNF667‐knockdown. ZNF667 upregulation attenuates leukocyte‐endothelial cell adhesion by negatively regulating the expression of P‐selectin in SIEA‐flap subjected to RIPC.

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Remote limb ischemic post-conditioning attenuates ischemia-reperfusion injury in rat skin flapby limiting oxidative stress

Cited by 11 publications

References 23 publications

Limb Remote Ischemic Conditioning: Mechanisms, Anesthetics, and the Potential for Expanding Therapeutic Options

Limb Remote Ischemic Conditioning: Mechanisms, Anesthetics, and the Potential for Expanding Therapeutic Options

Impact of groin flap ischemia-reperfusion on red blood cell micro-rheological parameters in a follow-up study on rats

ZNF667 attenuates leukocyte‐endothelial adhesion via downregulation of P‐selectin in skin flap following remote limb ischemic preconditioning

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