Penile reconstruction using mesenchymal stem cells

Laks, Marcus; Freitas-Filho, Luiz G.; Sayeg, Kleber; Leite, Mila Torii Correia; Teixeira, Luciana Cristina

doi:10.1590/s0102-865020150080000003

Cited by 7 publications

(7 citation statements)

References 32 publications

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“…In addition, these cells have the potential to form tumours in vivo 21 , thus restricting their applications. Among adult stem cells, mesenchymal stem cells (MSCs) have been the most widely studied and can be easily acquired by bone marrow aspiration 22 , 23 from individual patients. The most important characteristic of these cells is considered to be their capacity to maintain tissue integrity 24 .…”

Section: Introductionmentioning

confidence: 99%

Construction of engineered corpus cavernosum with primary mesenchymal stem cells in vitro

Xie

et al. 2017

Sci Rep

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Various methods have been used to reconstruct the penis. The objective of this study was to investigate the feasibility of constructing engineered corpus cavernosum with primary mesenchymal stem cells (MSCs) in a rabbit model in vitro. Acellular corporal matrices (ACMs) were obtained from adult rabbit penile tissues through an established decellularization procedure. MSCs were separated, purified, and then seeded on ACMs to construct engineered corpus cavernosum. The seeded ACMs were subsequently cultured in an incubator for 14 days. Histological analyses showed that MSCs seeded on the ACMs had proliferated and were well distributed. Detection of CD31, vWF, smooth muscle actin (SMA), and myosin protein as well as vWF and myosin mRNA revealed that the MSCs had differentiated into endothelial cells and smooth muscle cells. In addition, cell morphology of the engineered corpus cavernosum was directly observed by transmission electron microscopy. This study demonstrated that engineered corpus cavernosum could be successfully constructed using primary MSCs in vitro. This technology represents another step towards developing engineered corpus cavernosum in vitro.

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Section: Introductionmentioning

confidence: 99%

Construction of engineered corpus cavernosum with primary mesenchymal stem cells in vitro

Xie

et al. 2017

Sci Rep

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“…Higher VEGF expressing MDSC correlated with better distribution and growth of seeded cells on ACCM surface, higher number of nuclei, and cell adherence capacity. Also, ICP was significantly higher in this group (60% of normal), and markers for SMC and endothelium were expressed higher Laks et al (2015). used MSCs for reconstructing CC segments.…”

mentioning

confidence: 73%

“…Laks et al () used MSCs for reconstructing CC segments. Six rabbit ACCM were seeded with rabbit MSCs and implanted in excised CC space.…”

Section: Resultsmentioning

confidence: 99%

“…These stem cells migrate to and repopulate damaged organs, maintain homeostasis, and also suppress the immune response (Augello, Kurth, & De Bari, ). Laks et al () used undifferentiated MSCs and seeded them on ACCM and compared this to intravenous injection of MSCs. Both procedures resulted in filling of distal end of the CC and a one‐third ICP compared to normal.…”

Section: Discussionmentioning

confidence: 99%

“…Both procedures resulted in filling of distal end of the CC and a one‐third ICP compared to normal. Unfortunately, cavernosometry was only performed in one animal per group (Laks et al, ). Altogether, stem cells are a promising addition in regeneration and engineering of corporal tissue, but the ideal procedure resulting in normally functional corporal tissue has not yet been developed (Albersen, Lin, & Lue, ).…”

Section: Discussionmentioning

confidence: 99%

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A systematic review on cell‐seeded tissue engineering of penile corpora

Vocht

Kemp

Iljas

et al. 2017

J Tissue Eng Regen Med

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Tissue engineering of corporal tissue is a new development in otherwise untreatable erectile dysfunction and in urethral reconstructions to treat hypospadias or severe urethral stricture disease. Multiple complications can arise with the current treatments, whereas engineered tissue, if well vascularized and existing of autologous cells, may lead to better results. The aim of this review was to provide an overview of literature on cell-seeded-based tissue engineering of corporal penile tissue. A literature search was performed following the PRISMA guidelines. Papers describing cell-seeded tissue engineering of corporal tissue were included. Studies using different techniques, such as intracavernous injection were excluded. Fifteen articles were included in the review. Twelve of these studies described engineering of the corpus cavernosum in animal models. Two articles were found on engineering of animal corpus spongiosum and one article on engineering of the human glans. Both synthetic scaffolds and biological scaffolds were used. The advantage of a biological, acellular scaffold was that the native, complex architecture of corporal tissue was maintained. Most studies used endothelial and smooth muscle cells from corporal origin, but stem cells were also investigated. Furthermore, dynamic culturing achieved an improved cell content and functionality. This review has summarized the developments in tissue engineering of corpus cavernosum and spongiosum tissue. Functional tissue has been developed in animal studies with the use of seeded cells on scaffolds. This knowledge will form a basis for the development of tissue engineering of corporal tissue for clinical applications.

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Tissue-Engineered Approaches for Penile Reconstruction

Park¹

2020

Organ Tissue Engineering

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Penile reconstruction using mesenchymal stem cells

Abstract: The reconstruction of corpus cavernosum using descellularized matrices and mesenchymal stem cells, either by intravenous injection or directly seeded is possible, with growth of corpus cavernosum-like tissue.

Cited by 7 publications

References 32 publications

Construction of engineered corpus cavernosum with primary mesenchymal stem cells in vitro

Construction of engineered corpus cavernosum with primary mesenchymal stem cells in vitro

A systematic review on cell‐seeded tissue engineering of penile corpora

Tissue-Engineered Approaches for Penile Reconstruction

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