Antimicrobial activity of Melaleuca sp. oil against clinical isolates of antibiotics resistant Staphylococcus aureus

Abstract

PURPOSE:To extract the Melaleuca sp. oil and to assess its in vitro inhibitory effect against Staphylococcus aureus isolates obtained from lower limb wounds and resistant to several antibiotics. METHODS:A total of 14 test-tubes containing Mueller-Hinton broth were used to determine the Minimum Inhibitory Concentration (MIC). The following concentrations of the Melaleuca sp. oil were added to the first 11 tubes: 8; 4; 2; 1; 0.5; 0.2; 0.1; 0.05; 0.025; 0,0125 and 0.00625%. The 12 th and 13 th tubes, with and without oil, were used as the positive and negative controls, respectively. The experimental study was carried out in triplicate at 37ºC for 18 hours. The Minimum Bactericidal Concentration (MBC), able of killing all the microorganisms, was also determined. Two S. aureus isolates were obtained from lower limb wounds of female patients and the identification of the microorganisms (Staphylococcus aureus) and the test for susceptibility to the antimicrobial agents were carried out by automation using the apparatus MicroScan ® . After identification, the isolates were preserved in liquid Trypticase Soy medium, and inoculated for determination of the MIC and MBC. RESULTS:The MIC was 0.2% and the MBC was 0.4%. CONCLUSION:The Melaleuca sp. oil showed antimicrobial properties in vitro against strains isolated from lower limb wounds which were resistant to multiple antibiotics.

Comparative Antimicrobial Activity of Tea Tree Oil (Melaleuca Oil) and Common Topical Antimicrobials against Bacteria Associated With Wound and Topical Infections

“…In early studies, it was assumed that TTO was more active on antibiotic resistant bacteria, [14] which later on, were proved to be wrong assumptions [1]. The present study further proved the weakness of the present belief that resistance to antibiotic and sensitivity to TTO cannot be correlated [1,17] and thus the mechanism of bacterial resistance to TTO may not be similar to antibiotic drug resistance. The sensitivity of bacteria to TTO was strongly (p, ≤0.05) correlated with sensitivity to several antibiotics including novobiocin (r, 0.24), tetracycline (r, 0.22), gentamicin (r, 0.21), ciprofloxacin (r, 0.17), nitrofurantoin (r, 0.16), and chloramphenicol (r, 0.14).…”

“…In early studies on the antimicrobial activity of TTO, it was hypothesized to be more active against antibiotic-resistant bacteria [14] thus attracted considerable interest. Thereafter, several studies were conducted but only on a limited number of strains specifically using mupirocin-resistant and methicillin-resistant strains of Staphylococcus aureus (MRSA) and of other bacteria [15][16][17]. Most of the studies concluded an insignificant difference in TTO sensitivity of antibiotic-resistant and sensitive strains [1].…”

Control of canine idiopathic nasal hyperkeratosis with a natural skin restorative balm: a randomized double‐blind placebo‐controlled study

“…It can be hypothesized that the positive outcomes observed in this study resulted from the combination of the various active ingredients contained in DBB. The essential oil of M. cajuputi contains different molecules including 1.8‐cineole, with strong mucolytic, expectorant and anti‐inflammatory properties, and other monoterpenes and sesquiterpenes with antimicrobial and analgesic properties . Soybean oil is the main excipient of the product and contains vitamins (A, D, E and K) and essential fatty acids (linoleic, linolenic and oleic acids) .…”

A Review on the Potency of Melaleuca leucadendron Leaves Solid Waste in Wood Preservation and Its In Silico Prediction upon Biological Activities

“…Antibacterial. Cajuput oil, which was hydrodistilled from the leaves, was tested against the methicillin-resistant Staphylococcus aureus (MRSA) colony [42]. e minimum bactericidal concentration (MBC) and the minimum inhibition concentration (MIC) were found to be 0.2% and 0.4%, respectively.…”