Effects of vardenafil on the kidney of Wistar rats submitted to acute ischemia and reperfusion

Abstract

PURPOSE:To investigate the effect of vardenafil in kidney of rats submitted to acute ischemia and reperfusion. METHODS:Twenty-eight rats were randomly distributed into two groups. Right nephrectomy was performed and the vardenafil group received vardenafil solution (at a concentration of 1 mg/ml in 10 mg/kg) while the control group received 0.9% saline solution (SS) one hour prior to the ligature of the left renal pedicle. After one hour of ischemia, animals were submitted to twenty-four hours of reperfusion, followed by left nephrectomy. The kidney's histological parameters evaluated on the study included vacuolar degeneration and tubular necrosis. Apoptosis was assessed by immunohistochemistry for cleaved caspase-3 using the point-counting and digital methods (Cytophotometry). Also, a biochemical analysis for creatinine was conducted. RESULTS:There were statistically significant differences between groups only with regards to the vacuolar degeneration parameter and to the cleaved caspase-3 digital method. CONCLUSION:Vardenafil showed a protective effect on the kidney of rats subjected to acute ischemia and reperfusion in this model

Current Concepts on the Reno-Protective Effects of Phosphodiesterase 5 Inhibitors in Acute Kidney Injury: Systematic Search and Review

“…The main characteristics and results of the human studies evaluating the potential reno-protective effects of PDE5Is are summarized in Table 1 [17,24,38,39]. The main characteristics and results of the animal studies on currently proposed AKI models evaluating the potential reno-protective effects of sildenafil, tadalafil, icariin, vardenafil, zaprinast-udenafil are summarized in Table 2 [23,30,, Table 3 [29,35,45,49,[62][63][64][65][66][67][68][69][70][71][72][73][74], Table 4 [18,75,76], Table 5 [45,77,78], and Table 6 [21,40,79,80], respectively. The main characteristics and results of the animal studies in the AKI-CKD transition spectrum (focusing on renal function and/or structure alterations for up to three months, not fulfilling the KDIGO definition for CKD [6]) evaluating the potential reno-protective effects of sildenafil, tadalafil, icariin, vardenafil, zaprinast-udenafil are summarized in Table A1 [109,110], respectively (Appendix B).…”

Tadalafil protector effect during ischemia-reperfusion in rats

“…In renal ischemia, vardenafil demonstrated improved histological parameters and apoptosis in kidneys which underwent to 24-hour ischemia Reperfusion 15 .…”

The mechanism of attenuation of epithelial‐mesenchymal transition by a phosphodiesterase 5 inhibitor via renal klotho expression

“…Compared to placebo, 12‐week treatment with PF‐00489791 resulted in a significant reduction of 15.7% in the urinary albumin‐to‐creatinine ratio) . Results of several animal studies suggested that PDE5 inhibitors such as sildenafil, vardenafil, and tadalafil have a protective effect on renal ischaemic‐reperfusion injury . To demonstrate the protective effect of a PDE5 inhibitor on an impaired NO system in the kidney, we postulated that the central molecule between EMT and cGMP would be eNOS.…”

“…22 Results of several animal studies suggested that PDE5 inhibitors such as sildenafil, vardenafil, and tadalafil have a protective effect on renal ischaemic-reperfusion injury. [23][24][25][26] To demonstrate the protective effect of a PDE5 inhibitor on an impaired NO system in the kidney, we postulated that the central molecule between EMT and cGMP would be eNOS. However, eNOS expression in the kidney tissue did not show significant difference among groups in our pilot study.…”