Does alfa lipoic acid prevent liver from methotrexate induced oxidative injury in rats?

Abstract

PURPOSE:To determine the antioxidant and anti-inflammatory effects of alfa lipoic acid (ALA) on the liver injury induced by methotrexate (MTX) in rats. METHODS:Thirty two rats were randomly assigned into four equal groups; control, ALA, MTX and MTX with ALA groups. Liver injury was performed with a single dose of MTX (20 mg/kg) to groups 3 and 4. The ALA was administered intraperitonealy for five days in groups 2 and 4. The other rats received saline injection. At the sixth day the rats decapitated, blood and liver tissue samples were removed for TNF-α, IL-1β, malondialdehyde, glutathione, myeloperoxidase and sodium potassium-adenosine triphosphatase levels measurement and histological examination. RESULTS:MTX administration caused a significant decrease in tissue GSH, and tissue Na + , K + ATPase activity and which was accompanied with significant increases in tissue MDA and MPO activity. Moreover the pro-inflammatory cytokines (TNF-α, IL-β)were significantly increased in the MTX group. On the other hand, ALA treatment reversed all these biochemical indices as well as histopathological alterations induced by MTX. CONCLUSION:Alfa lipoic acid ameliorates methotrexate induced oxidative damage of liver in rats with its anti-inflammatory and antioxidant effects.

Protective effects of sea cucumber (Holothuria atra) extract on testicular dysfunction induced by immune suppressant drugs in Wistar rats

“…It has been shown that MTX showed renal and hepatic toxicity (Abdel‐Daim, Khalifa, Abushouk, Dkhil, & Al‐Quraishy, ). MTX is metabolised in liver and induced inflammation and liver damage and increased liver enzymes (Çakır et al., ). MTX metabolites have been confirmed to precipitate in kidney (Yuksel et al, ).…”

The effect of hippophae rhamnoides extract on oral mucositis induced in rats with methotrexate

“…No information was found in the literature about the role of IL-1β and TNF-α in MTX oral mucositis. However, Çakir, et al 7 (2015) reported that MTX induced the amount of MDA and IL-1β and TNF-α gene expressions in the kidney tissue. In our experiment, HRE, which significantly prevented the increase of MDA, also significantly prevented the increase of IL-1β and TNF-α gene expressions.…”

Protective effect of alpha‐lipoic acid against apical periodontitis‐induced cardiac injury in rats

“…The therapeutic effect of ALA has been the subject of research involving conditions such as atherosclerosis , joint diseases , AIDS , hepatic impairment , and diabetic polyneuropathy . Alpha‐lipoic acid has also been reported to be effective against conditions in which oxidative stress plays a role . It has also been reported that ALA might be a preventive agent in ulcerogenesis and inflammation‐associated tumor formation .…”