Effects of blocking αvβ3 integrin by a recombinant RGD disintegrin on remodeling of wound healing after induction of incisional hernia in rats

Abstract

Brazil. Conception, design, intellectual and scientific content of the study; analysis and interpretation of data; critical revision; final approval of the version to be published. ABSTRACT PURPOSE:To investigate the changes induced by DisBa-01 on repair of wound healing after induced incisional hernia (IH) in rats. METHODS:Thirty two male albino rats were submitted to IH and divided into four experimental groups: G1, placebo control; G2, DisBa-01-treated; G3, anti-α v β 3 antibodies-treated and G4, anti-α 2 antibodies-treated. Histological, biochemical and extracellular matrix remodeling analysis of abdominal wall were evaluated. RESULTS:After 14 days, 100% of the G2 did not present hernia, and the hernia ring was closed by a thin membrane. In contrast, all groups maintained incisional hernia. DisBa-01 also increased the number macrophages and fibroblasts and induced the formation of new vessels. Additionally, MMP-2 was strongly activated only in G2 (p<0.05). Anti-α v β 3 -integrin antibodies produced similar results than DisBa-01 but not anti-α 2 integrin blocking antibodies. CONCLUSION:DisBa-01 has an important role in the control of wound healing and the blocking of this integrin may be an interesting therapeutically strategy in incisional hernia.

A novel amphiphilic fluorescent probe BODIPY–O-CMC–cRGD as a biomarker and nanoparticle vector

“…8 showed that all the uorescent materials exhibited strong and stable uorescence signals via CLSM. Although the specicity of cRGD had already been reported, 18 the images presented in Fig. 8(A and B) did not reveal apparent specicity.…”

Hernia and Cancer: The Points Where the Roads Intersect

“…Moreover, the blockade with DisBa-01 may be preventive against incisional hernia development. A recent animal study from Brazil demonstrated that DisBa-01 increased the number of macrophages and fibroblasts and no subject treated with this recombinant disintegrin developed incisional hernia (117). The authors concluded that this might be a promising therapeutic tool in wound healing and incisional hernia prevention.…”

One-bead one-compound combinatorial library derived targeting ligands for detection and treatment of oral squamous cancer

“…To investigate the targeted receptor of LLY13, we employed an integrin antibody blocking assay [30, 31]. After HSC-3 oral cancer cells were treated separately with a panel of anti-integrin monoclonal antibodies (anti-α1, α2, α3, α4, α5, α6, αV, β1) prior to incubation with LLY13-beads, we found that only anti-α3 antibody could partially block HSC-3 cancer cell binding to LLY13-beads, indicating that the receptor for LLY13 might be α3 integrin.…”

“…Athymic nude mice ( nu / nu ) were obtained from Harlan (Hayward, CA, USA) at 5 to 6 weeks of age. OSC subcutaneous tumors were established by injecting HSC-3 (1 × 10 6 cells) into the right flank ( n = 20) [28, 30, 31]. To establish OSC orthotopic bioluminescent nude mice model in tongue, HSC-3 cells were transfected with lentiviral vectors that express green fluorescent protein (GFP)/firefly luciferase fusion proteins.…”