Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury

Abstract

. Design and supervised all phases of the study, critical revision. ABSTRACT PURPOSE:To investigate the effects of acute hyperglycemia on dexmedetomidine-induced preconditioning against renal ischemiareperfusion injury. METHODS:Sprague-Dawley rats were randomly arranged to the normoglycemic (NG) or hyperglycemic group (HG), with each group further divided into sham (no I/R injury), I/R (ischemia-reperfusion) and dex (given by dexmedetomidine) groups. Acute hyperglycemia was induced by intraperitoneal injection (i.p.) of 25% glucose (3 g/kg) 45 min before ischemia. Dexmedetomidine (50 μg/kg, i.p.) was administrated 30 min before induction of ischemia. Renal function, histology, apoptosis, expression of Bax, Bcl-2 and phosphorylated AKT (p-AKT) were detected. RESULTS:I/R insult significantly increased the serum levels of blood urea nitrogen and creatinine, apoptotic tubular epithelial cells, expression of Bax and p-AKT, but decreased Bcl-2 expression. All these changes were further enhanced by hyperglycemia (p<0.05). In hyperglycemic condition, there was no statistically difference between the I/R group and Dex group in all the aforementioned detection indexes (p>0.05). CONCLUSION:Acute hyperglycemia attenuates dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury in non-diabetic rats.

Proton Nuclear Magnetic Resonance (¹H-NMR)-Based Metabolomic Evaluation of Human Renal Allografts from Donations After Circulatory Death

“…A potential role for glucose metabolism in IR injury is suggested by the results of Chang et al [ 15 ], who found that Dapagliflozin, an antidiabetic inhibitor of sodium/glucose cotransporter 2 (SGLT2), could attenuate IR injury. Moreover, hyperglycemia has been shown to exacerbate kidney IR injury and accelerate renal dysfunction, with several molecular pathways being profoundly affected by the hyperglycemia that occurs prior to renal IR injury [ 16 , 17 ]. However, in the absence of samples and clinical measures from the donors, it was impossible to determine whether the glucose increase was due to IR injury or a prior insult.…”

Dexmedetomidine preconditioning inhibits the long term inflammation induced by renal ischemia/reperfusion injury in rats

“…Other studies also demonstrated that the administration with Dex could reduce the number of apoptotic tubular epithelial cells and attenuate renal tissue necrosis and histological lesions in a rat IRI model 7,8 . Besides, wang et al 23 aggravate the injuries caused by IRI. Si et al 25…”

Toll-like receptor 7 involves the injury in acute kidney ischemia/reperfusion of STZ-induced diabetic rats

“…Western blot was performed as previously described 23 . Protein concentrations were determined by densitometry values.…”