Innate and adaptive immunity gene expression of human keratinocytes cultured of severe burn injury

Abstract

. Conception, design, intellectual and scientific content of the study; analysis and interpretation of data; manuscript writing; critical revision. ABSTRACT PURPOSE:Evaluate the expression profile of genes related to Innate and Adaptive Immune System (IAIS) of human Primary Epidermal keratinocytes (hPEKP) of patients with severe burns. METHODS:After obtaining viable fragments of skin with and without burning, culture hKEP was initiated by the enzymatic method using Dispase (Sigma-Aldrich). These cells were treated with Trizol® (Life Technologies) for extraction of total RNA. This was quantified and analyzed for purity for obtaining cDNA for the analysis of gene expression using specific IAIS PCR Arrays plates (SA Biosciences). RESULTS:After the analysis of gene expression we found that 63% of these genes were differentially expressed, of which 77% were repressed and 23% were hyper-regulated. Among these, the following genes (fold increase or decrease): IL8 (41), IL6 (32), TNF (-92), HLA-E (-86), LYS (-74), CCR6 (-73), CD86 (-41) and HLA-A (-35).CONCLUSIONS: This study contributes to the understanding of the molecular mechanisms underlying wound infection caused by the burn. Furthermore, it may provide new strategies to restore normal expression of these genes and thereby change the healing process and improve clinical outcome.

Dual RNA-Seq Analysis of Trichophyton rubrum and HaCat Keratinocyte Co-Culture Highlights Important Genes for Fungal-Host Interaction

“…Although more elucidated in macrophages, the increased expression of this gene was also observed in keratinocytes of patients with severe burns, suggesting that this gene participates in the innate immune response in the presence of tissue injury [ 74 ]. Tissue damage also occurs in dermatophytoses as a result of the secretion of keratinolytic proteases by the fungus.…”

Sub-pathway analysis for severe burns injury patients: Identification of potential key lncRNAs by analyzing lncRNA-mRNA profile

“…In previous studies, the expression of various genes was found to be altered after severe burns. Noronha et al ( 4 ) found that several genes, including C-X-C motif chemokine ligand 8, interleukin 6, tumor necrosis factor and major histocompatibility complex, Class I, E played a contributory role in wound infection induced by burns. In addition, Padfield et al identified various genes that encode chemokines, complement, oxidative-stress and immune functions following severe burn trauma ( 5 ).…”

Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients

“…The high incidence of infection in burn patients is a major concern in clinical setting, because it is directly related to morbidity and mortality 6,7 .…”