Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats

Neto, Carlos Alberto da Silva Frias; Koike, Márcia Kiyomi; Saad, Karen Ruggeri; Saad, Paulo Fernandes; Montero, Edna Frasson de Souza

doi:10.1590/s0102-86502014001700004

Cited by 12 publications

(2 citation statements)

References 27 publications

(21 reference statements)

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“…Santos et al, investigating the protective effect of cilostazol on isolated rabbit femoral arteries, claimed that cilostazol has improved vascular reactivity after revascularization [ 5 ] . However, Neto et al found no beneficial effect of cilostazol on kidney and skeletal striated muscle in rats with IRI models [ 6 ] . In contrast, O’Donnell et al found positive effects of cilostazol on exercise-induced IRI in 90 patients with peripheral artery disease [ 7 ] .…”

Section: Introductionmentioning

confidence: 99%

Cardioprotective Effect of Cilostazol on Ischemia-Reperfusion Injury Model

Şahın¹,

Baytaroğlu²,

Sevgili³

2022

Braz J Cardiovasc Surg

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Introduction To clarify the potential protective role of cilostazol on rat myocardial cells with ischemia-reperfusion injury (IRI) models. Methods The study was conducted with three groups of 10 Wistar rats (control group, rats without any coronary ischemia; sham group, rats with coronary ischemia but without cilostazol administration; and cilostazol group, rats with coronary ischemia and cilostazol administration). The level of myocardial injuries was measured by analyzing cardiac troponin T and creatine kinase MB levels in blood samples. In tissue samples, adenosine triphosphate (ATP), nitric oxide, superoxide dismutase (SOD), and malondialdehyde were used to determine the amount of tissue damage. Tissues were stained with hematoxylin-eosin method, and samples were examined under light microscope. Results The mean level of ATP was 104.4 in the cilostazol group and 149.1 in the sham group ( P =0.044). SOD level was significantly higher in the cilostazol group than in the sham group (2075.3 vs . 1783.7, P =0.043). According to histopathological examination, all samples were classified as G0 in the control group. In the sham group, one sample was categorized as G1, six samples as G2, and three samples as G3. In the cilostazol group, nine samples and one sample were categorized as G1 and G2, respectively ( P =0.011). Conclusion Cilostazol has beneficial effects on Wistar rats’ myocardial cells in regard to decreasing inflammatory process, necrosis, and fibrosis. Our findings revealed that the use of cilostazol significantly decreased ATP and increased SOD levels in Wistar rats’ myocardial cells after IRI.

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Section: Introductionmentioning

confidence: 99%

Cardioprotective Effect of Cilostazol on Ischemia-Reperfusion Injury Model

Şahın¹,

Baytaroğlu²,

Sevgili³

2022

Braz J Cardiovasc Surg

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“…The histopathological evaluation of rat kidney and heart was done by two experienced histopathologists. For the quantification of the tissue damage, a scoring system was employed based on previously published criteria [14][15][16][17] . Collective histological scoring ranged from 0 (within normal range) to 5 (severe organ injury).…”

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confidence: 99%

Conditioning attenuates kidney and heart injury in rats following transient suprarenal occlusion of the abdominal aorta

Tsilimigras

Selemenakis

Vlachou

et al. 2020

Sci Rep

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Suprarenal aortic clamping during abdominal aortic aneurysm (AAA) repair results in ischemiareperfusion injury (IRI) in local (i.e. kidney) and distant (i.e. heart) tissue. To investigate perioperative approaches that mitigate IRI-induced tissue damage, Wistar rats underwent suprarenal aortic clamping either alone or in combination with short cycles of ischemic conditioning before and/or after clamping. Serum analysis revealed significant reduction in key biochemical parameters reflecting decreased tissue damage at systemic level and improved renal function in conditioned groups compared to controls (p < 0.05), which was corroborated by histolopathological evaluation. Importantly, the levels of DNA damage, as reflected by the biomarkers 8-oxo-G, γH2AX and pATM were reduced in conditioned versus non-conditioned cases. In this setting, NADPH oxidase, a source of free radicals, decreased in the myocardium of conditioned cases. Of note, administration of 5-HD and 8-SPT blocking key protective signaling routes abrogated the salutary effect of conditioning. To further understand the non-targeted effect of IRI on the heart, it was noted that serum TGF-β1 levels decreased in conditioned groups, whereas this difference was eliminated after 5-HD and 8-SPT administration. Collectively, conditioning strategies reduced both renal and myocardial injury. Additionally, the present study highlights TGF-β1 as an attractive target for manipulation in this context. Abdominal aortic aneurysm (AAA) is a potentially life-threatening entity, relatively common in routine clinical practice 1. Despite the introduction of endovascular aortic aneurysm repair (EVAR) as well as the significant improvements in surgical techniques, grafts, and perioperative care during the last decades, AAA repair still carries a considerable risk of morbidity and mortality 2. One of the main concerns in vascular surgery over the years

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Preconditioning improves muscle regeneration after ischemia–reperfusion injury

Zhong

Liu

Kim

et al. 2020

Journal Orthopaedic Research

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Ischemia-reperfusion injury (IRI) is a critical condition associated with serious clinical manifestations. Extensive research has focused on the strategies increasing organ tolerance to IRI. Preconditioning (PC) has been shown to provide protection to various organs toward IRI. However, the underlying mechanisms remain unknown. This study aimed to evaluate the role of PC on muscle regeneration after IRI and the potential underlying mechanisms. Three-month-old male UCP-1 reporter mice underwent unilateral hindlimb IRI with or without PC, the tissue viability and injury index were measured at 24 h after IRI. Hindlimb gait, muscle contractility, muscle histology were analyzed at 2 weeks after IRI. In another group of animals, β3 adrenergic receptor (β3AR) agonist amibegron and β3AR antagonist SR-59230A were administrated before PC/IRI, the hindlimb function and muscle regeneration were evaluated at 2 weeks after IRI. Our results showed that PC has little effect on improving the tissue viability at the acute phase of IRI, but it showed a long-term beneficial role of improving hindlimb function and muscle regeneration as evidenced by increased central nuclei regenerating myofibers. The effects of PC are related to inducing muscle fibro-adipogenic progenitor (FAP) brown/beige-like adipocyte (BAT) differentiation. Amibegron treatment displayed a similar role of PC while SR-59230A abolished the effect of PC. This study suggests PC has a beneficial role in promoting muscle regeneration after IRI through β3AR signaling pathwaystimulated FAP-BAT differentiation.

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Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats

Cited by 12 publications

References 27 publications

Cardioprotective Effect of Cilostazol on Ischemia-Reperfusion Injury Model

Cardioprotective Effect of Cilostazol on Ischemia-Reperfusion Injury Model

Conditioning attenuates kidney and heart injury in rats following transient suprarenal occlusion of the abdominal aorta

Preconditioning improves muscle regeneration after ischemia–reperfusion injury

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