Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation

Abstract

PURPOSE:To evaluate microscopic behavior and viability of dental pulp stem cells under glucose and glutamine deprivation. METHODS:Human tooth tissues were minced in isolated pieces and cultured until the desired cellular proliferation for experimental phases. Cells were cultured under variations of glucose and glutamine in both serum presence and absence, and then those cells were evaluated according to number and viability by MTT assay. The confocal microscopy analyzed cytoskeleton, nucleus, and mitochondria integrity. RESULTS:A low concentration of glucose favored cellular viability and microscopic behavior; the presence of glutamine in culture medium was favorable only when associated with glucose. The cellular biological potential in culture could be preserved in serum absence if nutritional requirements are adequate. CONCLUSION:Cell microscopic behavior and viability have demonstrated better patterns on serum-free low glucose culture medium with glutamine deprivation.

Development of regenerative and flexible fibroin‐based wound dressings

“…MTT assay confirmed the biocompatibility of both the SF films and the SF/glucose films, which provided an excellent platform for the proliferation of fibroblasts. Some researchers demonstrated that low concentrations of glucose improved cellular viability; furthermore, an in vitro study on the effect of glucose concentration on the growth of normal human dermal fibroblasts suggested that, when glucose is supplied to cells, the healing of chronic wounds is faster. The glucose requirement increases in cell in tissue repair, because glucose represents an energy source for cell proliferation and for the formation of extracellular matrix components …”

The likely role of proteolytic enzymes in unwanted differentiation of stem cells in culture

“…First, fetal bovine serum and culture media: the presence of fetal bovine serum in cell culture medium is questionable since there is a lack of characterization of these substances (growth factors), and the amount of such substances as well. Furthermore, the ideal culture media should contain standard nutrients in accordance with the cell type and necessity [5]. Second, the in vitro cell culture environment is very different from the in vivo environment of the source tissue, for instance: 3D environment, hormones and circulating growth factors [6].…”