Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis

Abstract

PURPOSE:To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis. METHODS:Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two or four weeks after intervention. Each group was divided into three subgroups according daily application of enemas containing saline, sucralfate at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis and neutral and acid mucins by Periodic Acid Schiff and Alcian Blue techniques, respectively. The contents of mucins were quantified by computer-assisted image analysis. Student's t paired and ANOVA test were used to compare the contents of both types of mucins among groups, and to verify the variance with time, establishing level of signification of 5% for both (p<0.05). RESULTS:Enemas containing sucralfate improves the inflammation and increases the tissue contents of neutral and acid mucins.The content of neutral mucins does not change with the time or concentration of sucralfate used, while acid mucins increases with concentration and time of intervention. CONCLUSIONS:Sucralfate enemas improve the inflammatory process and increase the tissue content of neutral and acid mucins in colon without fecal stream.

Claudin-3 and occludin content in the glands of colonic mucosa devoid from fecal stream submitted to topical intervention with oil extract of Curcuma longa

“…The surgical methodology used for the induction of exclusion colitis has already been described in previous studies published by our group [1][2][3] . Briefly, all animals were placed under general anesthesia by intramuscular administration of 0.1 mL/100 g of a 1:1 (v/v) ketamine (50 mg/mL) and xylazine (20 mg/mL).…”

“…The presence of colitis in the colon segments devoid of the fecal stream was confirmed by the presence of two independent histological parameters: mucosal-submucosal neutrophil infiltration and presence of epithelial erosion or ulceration and classified in crosses (-to 9+). The severity of the inflammation in the colonic mucosa devoid from the fecal stream was established in accordance with a previous used inflammatory grade scale 2 .…”

“…Several substances have been tested for the treatment of CDC. Most of these drugs exhibit anti-inflammatory and antioxidant effects reducing the epithelial inflammation and oxidative stress [1][2][3]12,13 . Curcumin, the principal active principle present in the rhizome of Curcuma longa, has showed to be a promising substance for the treatment of different groups of inflammatory bowel disease (IBD) because it's anti-inflammatory and antioxidant properties 14 .…”

“…This defense system is formed by different structures, which act as mechanical barrier, and by cells of the immune system, which constitute an immunological barrier [1][2][3] . The mechanical protection are formed by the mucus that recover the intestinal epithelium, the cellular membranes of colonocytes, the mechanisms of intercellular junctions (IJs) and basal membrane 4 .…”

“…zThese substances can reduce the epithelial damage, improve the inflammation and increase the mucous layer that recovers the colonic mucosa devoid of the fecal stream [1][2][3] . Curcumin has been used as a substance for the prevention and treatment of many organ and tissue disorders, most of which are associated with inflammation and oxidative stress 33,34 .…”