Histopathological evaluation of tumor necrosis and volume after cyanogenic chemotherapy

Abstract

PURPOSE:To determine the percentage of tumoral necrosis and volume after cyanogenic chemotherapy. METHODS:Histopathological findings of 20 Swiss mice inoculated subcutaneously in the left abdominal wall with 0.05 ml of cell suspension containing 2.5 x 10 5 viable cells of the Ehrlich tumor were evaluated. The tumor response to cyanogenic chemotherapy was determined using a system that comprises two inhibition factors of tumor growth by calculating the percentage of necrosis in the tumor tissue and calculation of tumor volume in treated animals relative to that in control animals. The importance of this system has been validated by the correlation between tumor inhibition in the groups treated with the respective percentages of necrosis. RESULTS:While the control group presented an average of 13.48 ± 14.71% necrosis and average tumor volume of 16.18 ± 10.94, the treated group had an average of 42.02 ± 11.58 and 6.8 ± 3.57, respectively. The tumor inhibition was significantly associated with treatment (p=0.0189). The analysis of necrosis percentage showed a significant prognostic importance (p=0.0001). CONCLUSION:It is concluded that the effect of cyanogenic chemotherapy showed strong inhibitory action of tumor growth, as well as an increase in its area of necrosis.

Action of Cyanogenic Substances and Magnetic Field in the Reduction of Neoplasms

“…When the exposure of tonsillin in tumor cells was verified, there was a concentration-dependent reduction in the number of tumor cells, and no sign of x-ray was demonstrated by the trip an blue test (MAKAREVIC ́ et al, 2014). Corroborating with Makarevic' et al (2014), Ramalho et al (2014), reported in his study that cyanidrin acetone, which is a cyanogenic substance, has a strong inhibiting action on tumor growth. Yuan et al (2018), observed that in the cell lines of neforblastoma and neuroblastoma, including G401, CHLA255 and N2a, after exposure to the magnetic field for 2 hours/day, the viability of the tumor cell decreases significantly after 2 days of magnetic field 2019), which reported in its review that tonsil in exerted antitumor effect on tumor cells of human colon cancer, where the cDNA result showed a significant difference in the expression of SNU-C4 cells after treatment at a dose of 5mg/ml for 24 hours.…”

“…In another study, it can be observed that the influence of cyanide, released by Acetone Cyanidrin (CA), on the treatment of Ehrlich tumor demonstrated that cyanide promoted an increase in necrosis and inhibited tumor growth when compared to the control group, again proving the difficulty of tumor cells in eliminating cyanide (RAMALHO et al, 2014). Tonsil in, can also be known as laetrile is a cyanogenic glycoside that belongs to the family Rosaceous, also known as vitamin B17, not being toxic, but produces a substance the HCN that at the dosage of 4g for 15 days can generate toxicity to the human body.…”

Комбінований Вплив Вінорельбіну З Наночастинками Оксиду Заліза Й Електромагнітного Випромінювання На Карциному Легені Льюїс

“…Отримані мікропрепарати фотографували і аналізували за допомогою мікроскопа OLYMPUS CХ 41 із цифровою камерою і програмним забезпеченням QuickPHOTO MICRO 2.3 за стандартних умов. Відсоток некротичних зон у тканині пухлини оцінювали згідно з [21].…”

Influência da estimulação elétrica nervosa transcutânea no crescimento de carcinoma 4T1 em glândula mamária de camundongos

“…Estes fatores são um importante estímulo para a migração e diferenciação de células imunes, portanto, um centro necrótico pode ser associado a um mau prognóstico (Onuchic e Chammas, 2010). Diversos autores utilizaram a hematoxilina e eosina para analisar os efeitos de diferentes intervenções na necrose tumoral como a quimioterapia cianogênica e a deleção da IL-33R, mas as diferenças metodológicas entre estes trabalhos e o presente estudo dificultam comparações (Ramalho et al, 2014;Milosavljevic et al, 2016).…”