Effects of hyperbaric oxygen therapy on the liver after injury caused by the hepatic ischemia-reperfusion process

Abstract

PURPOSE:To evaluate the effects of hyperbaric oxygen on rats submitted to hepatic ischemia and reperfusion. METHODS:Twenty-three Wistar rats were divided at random into 3 groups: SHAM, rats submitted to surgical and anesthetic stress without induction of hepatic ischemia/reperfurion; I/R, rats submitted to total ischemia of the hepatic pedicle for 25 min followed by 5 min of reperfusion; HBOI/R, rats submitted to 60 min of hyperbaric oxygen therapy at a pressure of 2 absolute atmospheres immediately after the experimental protocol of ischemia/reperfusion. Hepatic function was evaluated by quantitation of serum alanine aminotranferase (ALT) and aspartate aminotransferase (AST), and by mitochondrial function through the determination of states 3 and 4 of mitochondrial respiration, respiratory control ratio (RCR) and mitochondrial swelling. Data were analyzed by the Mann-Whitney test, with the level of significance set at p <0.05. RESULTS:There was a significant difference in state 3 values for the SHAM group vs I/R and I/R vs IRHBO, in state 4 values for the SHAM group vs I/R; and in mitochondrial swelling for the SHAM groups vs I/RHBO, SHAM vs I/R, and IR vs I/RHBO. CONCLUSION:The use of hyperbaric oxygen after I/R improved in a relative manner both the production of energy and the effects on the mitochondrial wall.

Hyperbaric Oxygen Therapy Ameliorates the Harmful Effects of Dexamethasone Induced Diabetes on Liver and Pancreas of Adult Male Albino Rats

“…Group IV rats revealed partial restoration of the normalarchitecture of the liver and structure of the hepatocytes. Also, it showed decreased connective tissue infiltration between the hepatic lobules and around the portal triad compared with group III.These findings were in agreement with other researchers [36,37] who reported that HBO treatment improved liver functions and stimulated hepatocyte regeneration in cases of liver cirrhosis. Also, our results were supported bythe work of [38,39] who demonstrated the protective effects of HBOT on the liver and some of them attributed these effects to the antiinflammatory and antioxidant action of HBOT.…”

Hyperbaric Oxygen Therapy in Liver Diseases

“…However, it is of importance to emphasize that only early HBOT given before neutrophil-mediated injury phase (in 3 hours after reperfusion) might take protective effect 11 . In addition, early HBOT after hepatic I/R injury also improved mitochondrial functions 12 . Besides, the therapeutic effect of HBOT in different periods of I/R was determined.…”

Effects of hyperbaric therapy on liver morphofunctional of rabbits (Oryctolagus cuniculus) after hind limb ischemia-reperfusion injury

“…Da Silveira et al . [ 23 ] stated that the supply of oxygen after reperfusion can act as a protector of mitochondrial membrane. Oxygen is an agent that plays a role in the formation of ATP by oxidative phosphorylation.…”