Effect of vitamin K1 supplementation on left colon healing in rats with extrahepatic biliary obstruction

Abstract

Intellectual and scientific content of the study, protocol design, provision of guidelines for surgical intervention, supervision of all phases of the study, manuscript writing, critical revision. ABSTRACT PURPOSE:To evaluate the effects of vitamin K1 on wound healing in the left colon of rats with experimental biliary obstruction. METHODS:Sixteen male rats, divided into four groups of four animals each (L, M, LK, and MK), underwent colostomy followed by bowel suture in the left colon. Seven days before, animals in the L and LK groups had undergone common bile duct ligation. The animals in groups MK and LK received vitamin K1 supplementation. On day 7 after bowel suture, repeat laparotomy was performed for evaluation of colonic healing by burst pressure measurement and collection of samples for histopathological analysis. Changes in body weight were evaluated in the four groups. RESULTS:Weight loss was lower in animals supplemented with vitamin K. No significant differences were observed in burst pressure among the four groups (p>0.05). Histological analysis showed more hemorrhage and congestion in the biliary obstruction groups.Supplemented animals exhibited increased collagen formation and less edema and abscess formation. CONCLUSION:Vitamin K supplementation attenuated weight loss and improved colonic wound healing in rats.

Effect of phylloquinone on indomethacin‐induced gastric ulceration in rats: Role of SIRT‐1

“…Phylloquinone (vitamin k1) is an essential macronutrient having anti‐inflammatory and antioxidant properties which was proved in previous studies 20,23,24 . The main target of our study is exploring the potential effects of phylloquinone against indomethacin‐induced gastric ulceration and studying the involved mechanisms.…”

“…Also, it inhibits inflammation by hindering NF‐κB and inducible nitric oxide synthase (iNOS) which are master regulators of the inflammatory process 21 Moreover, it effectively decreased hyperglycaemia in streptozotocin induced‐diabetes in rats 22 . Studies also showed that rats receiving phylloquinone show rapid healing of left colon 23 and that topical application of phylloquinone improves wound healing in rats 24 …”

Effect of vitamin K on wound healing: A systematic review and meta-analysis based on preclinical studies

“…By reading the full text for screening, seven articles were excluded (Supplementary Material S5) for the following reasons: four articles were not related to the effect of vitamin K on wound healing, and three articles did not report the wound healing rate, healing days, or tensile strength. Finally, six articles (including 16 studies) were eligible for meta-analysis (Amaral et al, 2014;Hemmati et al, 2014;Pinilla et al, 2014;Rush et al, 2016;Pazyar et al, 2019…”

“…The study of Hemmati et al (2014) and Amaral et al (2014) reported the wound tensile strength. The combined results suggested that there was no significant difference in wound tensile strength between vitamin K group and control group.…”

“…The included studies used Eucerin, 1% phenytoin, phosphatebuffered saline (PBS), etc. As controls, whereas blank controls were used in the studies ofAmaral et al (2014)) andPinilla et al (2014)…”