Reconstruction of abdominal wall defects using small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor

Abstract

. Design of the study, critical revision. ABSTRACT PURPOSE:To construct a new biomaterial-small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor, and to evaluate the new biomaterials for the reconstruction of abdominal wall defects. METHODS:Thirty six Sprague-Dawley rats were used in the animal experiments and randomly divided into three groups. The new biomaterial was constructed by combining small intestinal submucosa with gelatin hydrogel for basic fibroblast growth factor release. Abdominal wall defects were created in rats, and repaired using the new biomaterials (group B), compared with small intestinal submucosa (group S) and ULTRAPRO TM mesh (group P). Six rats in each group were sacrificed at three and eight weeks postoperatively to examine the gross effects, inflammatory responses, collagen deposition and neovascularization. RESULTS:After implantation, mild adhesion was caused in groups B and S. Group B promoted more neovascularization than group S at three weeks after implantation, and induced significantly more amount of collagen deposition and better collagen organization than groups S and P at eight weeks after implantation. CONCLUSION:Small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor could promote better regeneration and remodeling of host tissues for the reconstruction of abdominal wall defects.Key words: Abdominal Wall. Biocompatible Materials. Intestinal, Fibroblast Growth Factor 2. Rats. Reconstruction of abdominal wall defects using small intestinal submucosa coated withgelatin hydrogel incorporating basic fibroblast growth factor

The clinical application potential of extracellular matrix in cardiac tissue engineering

“…SIS-ECM coated with a gelatin hydrogel incorporating FGF-2, which was constructed by the same procedure as Tanaka and colleagues, also has been investigated in an abdominal wall defect rat model. 8 In this setting, a SIS-ECM patch incorporating FGF-2 showed better reconstruction of the abdominal wall compared with SIS-ECM alone. Taken together with this article by Tanaka and colleagues, a SIS-ECM patch incorporating FGF-2 could be an approach to myocardial regeneration because the prosthetic ECM materials and sustained protein therapy promote endogenous myocardial remodeling.…”

Emerging Trends in Abdominal Wall Reinforcement: Bringing Bio‐Functionality to Meshes

“…Indeed, in a mesh‐free incisional hernia model, the authors recorded a re‐herniation rate of 30% in the animals treated with a bFGF‐loaded rod, while it reached 90% for the control group . Following this first pilot study, other attempts have been conducted by directly loading the growth factor onto the surface of the meshes, by simple immersion or by entrapment in a vehicle, using for example gelatin hydrogel or collagen . Even though encouraging results using bFGF‐loaded meshes have been presented (Table ), a better control of the release of the active cue would clearly benefit long‐term implantation results.…”

“…Even though encouraging results using bFGF‐loaded meshes have been presented (Table ), a better control of the release of the active cue would clearly benefit long‐term implantation results. In the few publications investigating the bFGF release profile from the active mesh, it was shown that 50% of the growth factor diffused out within 10 days (under in vitro condition) or within only 2 days after subcutaneous implantation …”

Functional Augmentation of Naturally-Derived Materials for Tissue Regeneration

“…55,93 SIS matrix, which contains detectable levels of FGF and VEGF growth factors in addition to its structural protein content, has been functionalized with exogenous amounts of either protein to enhance abdominal wall defect repair. 48,75,123,129 Matrices produced from platelet-rich plasma (PRP) are rich in the host of growth factors, yet have been combined with recombinant proteins including nerve growth factor (NGF) and BMP. 22,138 PRP scaffolds, consisting of platelet-derived factors (e.g., PDGF, VEGF, transforming growth factor-β (TGF-β), and insulin-like growth factor (IGF)) present at physiological ratios within a fibrin network, are capable of stimulating cell proliferation, ECM production, and angiogenesis.…”