Comparative efficacy of Belzer or Euro-Collins solutions for pancreatic preservation during cold ischemic storage in rats

Abstract

PURPOSE:To compare the efficacy of different types of solutions (Belzer or Euro-Collins) for the preservation of rat pancreas during cold ischemia. METHODS:Thirty Wistar rats were divided into three groups according to the perfusion or storage solution: Group E (perfusion and storage in Euro-Collins solution); Group B (perfusion and storage in Belzer solution) and Group BE (Perfusion in Belzer solution and storage in Euro-Collins solution). After perfusion, the pancreas was excised and stored at 4˚C for 18 hours. Amylase was measured at 6, 12 and 18h, and histological analysis of the pancreas was performed after 18h of cold storage. RESULTS:Amylase was elevated and comparable in Groups E and BE after 12 and 18 hours of ischemia (p<0.05). In the exocrine pancreas, histological differences in the amount of necrosis (p=0.049), lymphocytic infiltrate (p<0.001) and neutrophilic infiltrate (p=0.004) were observed, with more favorable features present in Group B. In the endocrine pancreas, Group B showed less edema (p<0.001), but other parameters were similar among all groups. CONCLUSION:The Euro-Collins solution is inferior to the Belzer solution for the preservation of rat pancreas during cold ischemia.

A new method to measure intestinal secretion using fluorescein isothiocyanate-inulin in small bowel of rats

“…With this, we were able to demonstrate the reproducibility of the system and the ability to continuously collect samples of the luminal effluent. Also, this documents [13,14,26]. Absorption or secretion of fluid by the intestine will lead to an increase and/or decrease in micromolar concentration of the FITC-inulin, which is measured by nanfluorospectrometer over time.…”

Interleukin-1 Receptor-Induced Nitric Oxide Production in the Pancreas Controls Hyperglycemia Caused by Scorpion Envenomation

“…In fact, NO impacts mitochondrial oxidative phosphorylation, which, in turn, inhibits insulin secretion [21]. Mild edema was also observed in β-cells of islets with notable leukocyte infiltration, which has been shown to reduce insulin secretion in different mouse models [23][24][25][26]. This study speculated that the inflammatory infiltrate in the pancreas contributes to increased IL-1 production, whereas IL-1/IL-1R signaling disrupts β-cell homeostasis via the induction of NO.…”