Severe autogenously fecal peritonitis in Wistar rats with permanent bilateral carotid occlusion. Response to intra peritoneal moxifloxacin combined with dexamethasone

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PURPOSE:To investigate the morphological aspects of brain and eyes in newborn rats whose mother underwent autogenously fecal peritonitis. METHODS:Four pregnant rats that underwent fecal peritonitis, with a 10% fecal suspension in dose of 4 ml per kilogram received two antimicrobial treatments: 1. intraperitoneal moxifloxacin and dexamethazone; and 2. Intravenous meropenem. After head inspection, the brain consistencies and the eyes belonging to all offspring were analyzed. RESULTS:The brains of newborn from rats that received 4 ml/kg of 10% suspension of feces showed, significantly smaller and less than the firm consistency of those in the control group. Congenital cataract was observed in 9 (34.6%). No cataract was observed in the 20 newborn rats from the mothers that received the combination of moxifloxacin and dexamethasone. Cataract could be observed in three (13.6%) offspring from mothers that received meropenem. CONCLUSIONS:Peritonitis can produce brain damage and congenital cataract in rats. The translation to humans is that intra abdominal infection in pregnant women may be associated with damage in brain and eye structures of their concepts. This can be averting using the adequate early therapeutically approach.

Section: Discussionmentioning

confidence: 99%

Brain damage and congenital cataract due to autogenously fecal peritonitis in pregnant Wistar rats

Brandt

Melo

Gadelha³

et al. 2014

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“…In these papers the rats included in the investigation weighed 300g to 330g, however there was no mention to the ages of the rats in some studies 5,6 , and in other the rats were very young (between two and three months). In the present study it was followed a previous protocol 16 , in which it was produced a severe peritonitis and sepsis, but the rats were young (around 12 month-old). One limitation of this investigation was not foreseeing that the 6 ml/kg of 10% autogenously fecal suspension for producing severe peritonitis was an overwhelming infectious insult that the older rats could not overcome for the first 48 h post peritonitis induction, thus not allowing true comparison with the other two young rat groups that received intravenously meropenem after seven days post peritonitis induction.…”

Section: Discussionmentioning

confidence: 99%

Severe autogenously fecal peritonitis in ageing Wistar rats. Response to intravenous meropenem

Mascena

Melo²,

Gadelha³

et al. 2014

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PURPOSE:To evaluate the treatment outcome of severe peritonitis in rats with increasing age. METHODS:Thirty Wistar rats stratified in three groups: group I -six month-old; group II -12 month-old; and group III -18 monthold, underwent autogenously fecal peritonitis (6 ml/kg rat), and were treated with intravenous meropenem. The survival animals were followed-up for 45 days. The variables were expressed by their mean and standard error of the mean (SEM). p<0.05 was used for rejecting the null hypothesis. The study was approved by the Ethics Committee. RESULTS:There was a significant increase in the mortality and morbidity in elderly rats. Of interest, even among young survival rats presenting with severe residual abscesses both in the abdomen and thorax cavities, they present an almost normal life. CONCLUSIONS:The treatment of severe autogenously fecal peritonitis with intravenous meropenem reached reasonable results in rats with six and twelve months of age, even considering residual abscesses on abdomen and thorax cavities. However, the great majority (80%) of elderly rats could not overcome the initial severe infectious challenge, proving that ageing is a very important risk factor for impairing immune response. Thus, sepsis remains a challenging situation, especially in elderly.

“…The morbidity scores, according to previous protocol 13 , of the these rats were respectively: 7, 8, 7 and 8. All four survival rats were living normal life without any symptom or sign of infection.…”

Section: Alcohol Extract Of Schinu Sterebinthifolius Raddi (Anacardiamentioning

confidence: 98%

“…It was injected 5 ml/kg of filtered 10% fecal suspension in the rat left lower quadrant, according with previous protocol 13 . After 24 hours, in the six survival rats it was injected.…”

Section: Eight Male Wistar (Rattus Norvegicus Albinus Rodentiamentioning

confidence: 99%

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Alcohol extract of Schinu sterebinthifolius raddi (anacardiaceae) as a local antimicrobial agent in severe autogenously fecal peritonitis in rats

Melo

Gadelha²,

Oliveira

et al. 2014

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PURPOSE:To develop an alcoholic extract of the inner bark of the Schinus terebinthifolius raddi and to test its impact on autogenously fecal peritonitis in Wistar rats. METHODS:The inner bark of the Schinus terebinthifolius raddi was kept for seven days in 70% ethanol alcohol. The total elimination of the solvent was performed in a rotary evaporator under reduced pressure at 55-60°C. Four milliliter of this extract was injected, after 24 h, into the abdominal cavity of six out of eight survival rats that underwent autogenously fecal peritonitis with five milliliter of 10% filtered fecal suspension. They were clinically followed up for 45 days when they were euthanized. The necropsy findings (inventory) of the abdominal and thorax cavities were inspected and the main findings were recorded and photographed. The investigation was approved by the Ethics Committee. RESULTS:Two out of six survival rats that were critically ill after 24 h died within the 12 h after the extract injection into the abdominal cavity. Four rats that were also critically ill recovered and gradually became healthy, eating well, regaining weight and moving normally in the cage. At 45 days post severe peritonitis the necropsy findings revealed few signs of residual infection on the abdominal and thorax cavities. There were no bowel adhesions. CONCLUSION:The impact of alcoholic extract of the inner bark of the Schinus terebinthifolius raddi was considered very positive and promising as natural local antiseptic against very severe peritonitis in Wistar rats.