Cutaneous changes in rats induced by chronic skin exposure to ultraviolet radiation and organophosphate pesticide

Fernandes, Tânia Rita Moreno de Oliveira; Santos, Itamar; Korinsfky, Juliana Pedrosa; Silva, Benedyto Savio Lima e; Carvalho, Luiz Otávio Pereira; Plapler, Hélio

doi:10.1590/s0102-86502014000100002

Cited by 6 publications

(3 citation statements)

References 22 publications

(53 reference statements)

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“… 66 Moreover, some studies revealed the skin changes implicated with UVB exposure, where hyperkeratosis and acanthosis are among the common alterations in skin morphology after irradiation. 67 , 68 Another important factor that causes skin damage is the oxidative stress that depletes vitamin C levels in the skin, 39 , 40 which is known to stimulate collagen synthesis and protect against UV-induced photodamage. According to the results, the group that was treated with collagen- and Vit C-loaded NEs mixture showed the most healthy skin features, as collagen was found to elevate the degree of hydration at the inner layers of the skin through the topical treatment with cosmetic formulations containing collagen peptides, 69 and boost the smoothness and the luminosity of the skin surface at the microtextural level.…”

Section: Discussionmentioning

confidence: 99%

A Cosmeceutical Topical Water-in-Oil Nanoemulsion of Natural Bioactives: Design of Experiment, in vitro Characterization, and in vivo Skin Performance Against UVB Irradiation-Induced Skin Damages

Yousry¹,

Saber²,

Abd-Elsalam³

2022

IJN

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Damage to human skin occurs either chronologically or through repetitive exposure to ultraviolet (UV) radiation, where collagen photodegradation leads to the formation of wrinkles and skin imperfections. Consequently, cosmeceutical products containing natural bioactives to restore or regenerate collagen have gained a remarkable attention as an ameliorative remedy. Methods: This study aimed to develop and optimize collagen-loaded water-in-oil nanoemulsion (W/O NE) through a D-optimal mixture design to achieve an ideal multifunctional nanosystem containing active constituents. Vit E was included as a constituent of the formulation for its antioxidant properties to minimize the destructive impact of UV radiation. The formulated systems were characterized in terms of their globule size, optical clarity, and viscosity. An optimized system was selected and evaluated for its physical stability, in vitro wound healing properties, and in vivo permeation and protection against UV radiation. In addition, the effect of collagen-loaded NE was compared to Vit C-loaded NE and collagen-/Vit C-loaded NEs mixture as Vit C is known to enhance collagen production within the skin. Results: The optimized NE was formulated with 25% oils (Vit E: safflower oil, 1:3), 54.635% surfactant/cosurfactant (Span 80: Kolliphor EL: Arlasolve, 1:1:1), and 20.365% water. The optimized NE loaded with either collagen or Vit C exhibited a skin-friendly appearance with boosted permeability, and improved cell viability and wound healing properties on fibroblast cell lines. Moreover, the in vivo study and histopathological investigations confirmed the efficacy of the developed system to protect the skin against UV damage. The results revealed that the effect of collagen-/Vit C-loaded NEs mixture was more pronounced, as both drugs reduced the skin damage to an extent that it was free from any detectable alterations. Conclusion: NE formulated using Vit E and containing collagen and/or Vit C could be a promising ameliorative remedy for skin protection against UVB irradiation.

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Section: Discussionmentioning

confidence: 99%

A Cosmeceutical Topical Water-in-Oil Nanoemulsion of Natural Bioactives: Design of Experiment, in vitro Characterization, and in vivo Skin Performance Against UVB Irradiation-Induced Skin Damages

Yousry¹,

Saber²,

Abd-Elsalam³

2022

IJN

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“…It allows the verification of the feasibility of the planned formulation for use in other pharmacological models and preclinical trials. Mice and rats are routinely used as an experimental tool to study novel bioactive medicines and healing remedies (Surey et al, 2014;Fernandes et al, 2014;Sá et al, 2012;Magalhães et al, 2010). The Wistar rats were chosen for this study for being small, easy to purchase and due to the standardization concerning age, weight, sex, food, accommodation, cleaning care and experimental manipulation.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the cicatrizant activity of a semisolid pharmaceutical formulation obtained from Platonia insignis Mart.

Monica¹,

Joselma²,

Rezende³

et al. 2015

Afr. J. Pharm. Pharmacol.

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The use of natural products for skin treatments has been increased. Mainly, those of topical action incorporated to pharmaceutical forms, since they allow the restoring of skin integrity after possible attacks. In this context, the plant Platonia insignis Mart has been widely used for the treatment of various skin diseases and as wound healing medicine in folk medicine. The aim of this study is to evaluate the wound healing activity of a cream formulated containing triglyceride isolated from P. insignis Mart called 1,3-distearoyl-2-oleoylglycerol (TG1) in skin lesions induced in Wistar rats by macroscopic and histological analysis of the wounds. The animals were randomly divided into 5 groups according to the treatment to be performed. The negative control received saline solution, the positive control received collagenase and the other groups received cream containing the triglyceride at concentrations of 5, 10 and 15%. The results showed effectiveness in the healing of wounds through reduction of their diameter compared to negative control. Histological analysis demonstrated the inflammatory and proliferative phenomena of healing in wounds of rats. The cream with TG1 in three concentrations demonstrated efficacy in wound healing, as evidenced by macroscopic and microscopic analyses of lesions in Wistar rats. Based on this, a further development of phytomedicines for wound care is suggested.

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“…One of the major molecular mediators of photoimmunosuppression is UVR-induced DNA damage ( 3 ). It has also been shown that chronic UVR can lead to the induction of skin cancer, which is the most serious adverse health effect of UVR ( 4 ). Excess sun exposure increases the risk of non-melanoma and melanoma skin cancer ( 5 , 6 ).…”

Section: Introductionmentioning

confidence: 99%

Prevention of ultraviolet radiation-induced immunosuppression by sunscreen in Candida albicans-induced delayed-type hypersensitivity

Chen

Zhao

et al. 2016

Molecular Medicine Reports

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Ultraviolet (UV) radiation-induced immunosuppression leading to skin cancer has received increased attention in previous years. The present study aimed to investigate the immunoprotection offered by Anthelios sunscreen in a mouse model of Candida albicans-induced delayed-type hypersensitivity. Anthelios sunscreen was applied to the skin on the dorsal skin of BALB/c mice treated with a sub-erythema dose of solar-simulated radiation. Delayed-type hypersensitivity was induced by immunization with Candida albicans. Changes in the skin thickness of the foot pads were measured, and immunosuppression rates were also evaluated. The expression levels of CD207, CD80 and CD86 in the Langerhans cells were semi-quantitatively detected using Western blotting and immunohistochemical assays. The delayed-type hypersensitivity mouse model was successfully established. The minimal erythema doses of UVA and UVB exposure to the mice were 2,000 and 145 mJ/cm2, respectively. The immunosuppression rates in the sunscreen group and non-sunscreen group were 24.39 and 65.85%, respectively (P<0.01). The results of the Western blotting and immunohistochemistry showed that the expression levels of CD207 (P<0.01), CD80 (P<0.05) and CD86 (P<0.01) were higher in the sunscreen group, compared with those in the non-sunscreen group. UV exposure reduced Candida albicans antigen-induced delayed-type hypersensitivity. Anthelios sunscreen was found to protect the skin from immunosuppression through the activation of epidermal Langerhans cells.

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Cutaneous changes in rats induced by chronic skin exposure to ultraviolet radiation and organophosphate pesticide

Abstract: Concurrent association of UVR-B to organophosphate pesticide produced more severe lesions microscopically, although this has not been so apparent macroscopically. In daily practice the clinical evaluation should be complemented with laboratory evaluation.

Cited by 6 publications

References 22 publications

A Cosmeceutical Topical Water-in-Oil Nanoemulsion of Natural Bioactives: Design of Experiment, in vitro Characterization, and in vivo Skin Performance Against UVB Irradiation-Induced Skin Damages

A Cosmeceutical Topical Water-in-Oil Nanoemulsion of Natural Bioactives: Design of Experiment, in vitro Characterization, and in vivo Skin Performance Against UVB Irradiation-Induced Skin Damages

Evaluation of the cicatrizant activity of a semisolid pharmaceutical formulation obtained from Platonia insignis Mart.

Prevention of ultraviolet radiation-induced immunosuppression by sunscreen in Candida albicans-induced delayed-type hypersensitivity

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