Corticosteroid effect upon intestinal and hepatic interleukin profile in a gastroschisis rat model

Gonçalves, Frances Lilian Lanhellas; Bittencourt, Daniel Guimarães; Velloso, Lı́cio A.; Schmidt, Augusto F.; Gallindo, Rodrigo Melo; Sbragia, Lourenço

doi:10.1590/s0102-86502013001300003

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…We have recently shown that DEX can significantly increase intestinal permeability and tight junction expression in small intestine of broiler chickens (Barekatain, Nattrass, et al, 2019). Although DEX is known as an anti‐inflammatory drug, its effect can be pro‐inflammatory in different organs including the intestine depending on dose and physiological conditions (Gonçalves et al., 2013).…”

Section: Discussionmentioning

confidence: 99%

Expression of selected genes encoding mechanistic pathways, nutrient and amino acid transporters in jejunum and ileum of broiler chickens fed a reduced protein diet supplemented with arginine, glutamine and glycine under stress stimulated by dexamethasone

Barekatain

Chrystal

Gilani

et al. 2020

Animal Physiology Nutrition

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Reducing crude protein and supplementation with synthetic amino acids in poultry nutrition is a recent trend to avoid wastage of protein and ammonia in production systems. Stress has been shown to impair intestinal barrier and increase inflammatory response. This study was performed on intestinal tissues of broiler chickens to understand the mechanism of stress induced by a synthetic glucocorticoid, dexamethasone (DEX) and the effect of supplementation of arginine, glutamine and glycine in reduced protein diets. Intestinal tissue samples from a previous study were utilized. Male Ross 308 chickens received a basal diet for the first seven days and then fed with crude protein that was reduced to 194 g/kg in grower experimental diets supplemented with glutamine, glycine and additional arginine at 10, 10 and 5 g/kg respectively. Half of the 96 individual birds were injected with DEX (0.5 mg/kg body weight) or saline on days 14, 16, 18 and 20 of age. mRNA expression for jejunum and ileum for amino acid transporters (y+LAT‐1, Bo,+AT, EAAT‐3 and CAT‐1), mechanistic genes (SGLT‐1, mTOR, IAP and FABP‐2) and pro‐inflammatory genes (MUC‐2, NF‐κB, iNOS, IL‐8 and IL‐1β) were analysed using real‐time PCR. The results showed that DEX decreased y+ LAT1 in jejunum, Bo,+AT and EAAT‐3 in ileum. Arginine increased CAT‐1 in the jejunum and ileum under DEX treatment. Through an interaction, DEX reduced IAP in jejunum of glycine and arginine supplemented group and reduced mTOR in jejunum independently. DEX reduced MUC‐2 and iNOS in jejunum and increased iNOS and IL8 in the ileum. Amino acid supplementation did not appear to ameliorate these effects; however, there were some positive effects of glycine on NF‐κB and arginine through increased CAT‐1. Mechanistic understanding of amino acid supplementation in broiler diets warrants further research particularly when dietary protein is reduced below the level tested in the present study.

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Section: Discussionmentioning

confidence: 99%

Expression of selected genes encoding mechanistic pathways, nutrient and amino acid transporters in jejunum and ileum of broiler chickens fed a reduced protein diet supplemented with arginine, glutamine and glycine under stress stimulated by dexamethasone

Barekatain

Chrystal

Gilani

et al. 2020

Animal Physiology Nutrition

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“…Corticosteroids usage such as Betamethasone, a potent anti-inflammatory agent acting as inhibitor of pro-inflammatory agents and some interleukins (IL-1, IL-6 e IL-10), can also act locally inhibiting inflammatory reactions described in the previous study [13].…”

Section: Introductionmentioning

confidence: 84%

Comparative Study of Histological Alterations in Intravascular Catheter Region with and without the Use of Local Corticosteroid

Muniz¹,

Amaral²,

Novo³

et al. 2017

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“…84,85 Similar findings of ICC were also observed by Auber et al 86 França et al found similar results with α-internexin marker showing more immature plexus in G exposed in different GA (18.5 and 19.5 days) and concluded that the time of exposure of AF increased the ID. 87,88 Yu et al and Bittencourt et al performed antenatal treatment with dexamethasone and found decreased wall thickening 89,90 with increase of intestinal IL-6 and decrease of tumor necrosis factor-α, 91 which may be stimulated by the activation of hepatic NF-κB that was increased in GS. 92 It appears that those inflammatory responses have some implication in compromising fetal insulin receptors and glucose uptake that may induce transitory insulin resistance and promote intrauterine growth restriction.…”

Section: Gastroschisis Model Surgically Created In Ratmentioning

confidence: 99%

Gastroschisis: State of the Art in Translating Experimental Research to the Bedside

et al. 2019

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The survival rate of newborns with gastroschisis (GS) has been increasing in the past decades; however, the morbidity continues to be high, mainly related to the length of hospitalization due to disturbances of motility, absorption, and risk of intestinal infections. The development of basic research with the creation of experimental models has provided enormous advances in the understanding of the pathophysiology of the disease. These models allowed the study of the target genes involved in the embryology of the defect, the influence of the amniotic fluid, and the use of drugs and fetal therapies in an attempt to reduce the intestinal damage and to provide a rapid return of intestinal motility. Our aim was to describe the main GS models and the translational, historical impact of these research advances on the disease.

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Corticosteroid effect upon intestinal and hepatic interleukin profile in a gastroschisis rat model

Cited by 5 publications

References 12 publications

Expression of selected genes encoding mechanistic pathways, nutrient and amino acid transporters in jejunum and ileum of broiler chickens fed a reduced protein diet supplemented with arginine, glutamine and glycine under stress stimulated by dexamethasone

Expression of selected genes encoding mechanistic pathways, nutrient and amino acid transporters in jejunum and ileum of broiler chickens fed a reduced protein diet supplemented with arginine, glutamine and glycine under stress stimulated by dexamethasone

Comparative Study of Histological Alterations in Intravascular Catheter Region with and without the Use of Local Corticosteroid

Gastroschisis: State of the Art in Translating Experimental Research to the Bedside

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