Biocompatibility of Ferrara intracorneal ring segment with and without chondroitin sulfate coating: clinical and histopathological evaluation in rabbits

Andreghetti, Eduardo; Hashimoto, Mitsuo; Domingues, Maria Aparecida Custódio; Antunes, Vitor Andrigheti Coronado; Segundo, Paulo de Souza; Silva, Maria Rosa Bet de Moraes

doi:10.1590/s0102-86502013000900002

Cited by 5 publications

(4 citation statements)

References 26 publications

(25 reference statements)

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“…The increase in desmoplakin expression could correspond to the stimulation of epithelial mitosis. Moreover, epithelial hypoplasia in the epithelium covering the rings has been observed, whilst the central epithelium retains a normal structure ( 61 , 62 ). The epithelium between these two zones is hypertrophic ( 62 ), which is related to the biological stress produced by the implant ( 60 ).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, epithelial hypoplasia in the epithelium covering the rings has been observed, whilst the central epithelium retains a normal structure ( 61 , 62 ). The epithelium between these two zones is hypertrophic ( 62 ), which is related to the biological stress produced by the implant ( 60 ). In fact, on removing the rings a recovery of the epithelium is observed ( 61 ).…”

Section: Discussionmentioning

confidence: 99%

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Tear proteome profile in eyes with keratoconus after intracorneal ring segment implantation or corneal crosslinking

et al. 2022

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PurposeKeratoconus (KC) is a corneal ectasia characterized by structural changes, resulting in progressive thinning and biomechanical weakening that can lead to worsening visual acuity due to irregular astigmatism. Corneal collagen Crosslinking (CXL) and Intracorneal Ring Segment (ICRS) are widely used treatments in KC disease, but the alterations they cause in biomechanical mediators are still poorly understood. The aim of this study was to analyze the tear proteome profile before and after treatments to identify biomarkers altered by surgery.Materials and methodsAn observational, prospective, case-control pilot study was conducted, analyzing tear samples from KC patients by nano-liquid chromatography-mass spectrometry (nLC-MS/MS). Data are available via ProteomeXchange with identifier PXD035655. Patients with KC who underwent ICRS surgery (n = 4), CXL (n = 4), and healthy subjects (Ctrl, n = 4) were included in this study. Clinical parameters were measured and tear samples were collected before and 18 months after surgery. Proteins with ≥2 expression change and p-value < 0.05 between groups and times were selected to study their role in post-operative corneal changes.ResultsThese analyses led to the identification of 447 tear proteins, some of which were dysregulated in KC patients. In comparisons between the two surgical groups and Ctrls, the biological processes that were altered in KC patients at baseline were those that were dysregulated as a consequence of the disease and not of the surgical intervention. Among the biological processes seen to be altered were: immune responses, cytoskeleton components, protein synthesis and metabolic reactions. When comparing the two treatment groups (ICRS and CXL), the process related to cytoskeleton components was the most altered, probably due to corneal thinning which was more pronounced in patients undergoing CXL.ConclusionThe changes observed in tears after 18 months post-operatively could be due to the treatments performed and the pathology. Among the deregulated proteins detected, A-kinase anchor protein 13 (AKAP-13) deserves special attention for its involvement in corneal thinning, and for its strong overexpression in the tears of patients with more active KC and faster disease progression. However, it should be kept in mind that this is a pilot study conducted in a small number of patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Tear proteome profile in eyes with keratoconus after intracorneal ring segment implantation or corneal crosslinking

et al. 2022

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“…Intrastromal rings are implanted in the deep corneal stroma to modify the corneal curvature and are classically made of PMMA. Paraffin-based histology is generally chosen for these implants (Andreghetti et al 2013) and a double stain with hematoxylin and eosin and periodic acid of Schiff allows for adequate corneal staining. Histopathologic evaluation should focus on the thickness of the corneal epithelium that can be adversely thinned due to the presence of the implant.…”

Section: Implanted Ocular MDmentioning

confidence: 99%

Ocular Medical Devices: Histologic Technique and Histopathologic Evaluation of the Biocompatibility and Performance

Drevon-Gaillot

2018

Toxicol Pathol

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Ocular medical devices (MDs) represent a very wide and promising field of human ophthalmology. In preclinical studies evaluating the safety and/or performance of these ocular MDs, the choice of histologic technique and the focus of the histopathologic evaluation method should take into consideration the following aspects: the specific guidelines possibly associated with the MD or combination product, the ocular compartment in contact with the MD and its specificities, and last the nature of the biomaterial used in the MD. Following a brief overview of animal models, this short review will present the different types of ocular MDs and will present the specificities of the histologic technique and the histopathologic evaluation related to ocular MDs.

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“…The results obtained have shown the absence of toxicity in experimental material samples and their low adhesive properties with respect to the stromal cell culture, thereby confirming its potential suitability for intracorneal implantation. так и за рубежом [10][11][12][13]. Примечательным явля ется успешное использование данного материала в виде интрастромальных роговичных сегментов, которые обычно имплантируются в относительно толстую периферическую часть роговицы [14].…”

Section: актуальностьunclassified

Investigation on the intracorneal lens material biocompatibility using the model of the corneal stromal cell culture

Малюгин¹,

Борзенок²,

Mushkova³

et al. 2017

RJTAO

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Biocompatibility of Ferrara intracorneal ring segment with and without chondroitin sulfate coating: clinical and histopathological evaluation in rabbits

Abstract: Ferrara intracorneal rings coated with chondroitin sulfate (FICRS-CS) caused lower frequency of clinical and histopathological alterations than Ferrara intracorneal rings without the coating (FICRS), demonstrating higher biocompatibility of the FICRS-CS.

Cited by 5 publications

References 26 publications

Tear proteome profile in eyes with keratoconus after intracorneal ring segment implantation or corneal crosslinking

Tear proteome profile in eyes with keratoconus after intracorneal ring segment implantation or corneal crosslinking

Ocular Medical Devices: Histologic Technique and Histopathologic Evaluation of the Biocompatibility and Performance

Investigation on the intracorneal lens material biocompatibility using the model of the corneal stromal cell culture

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