Effects of acupuncture and electroacupuncture on estradiol-induced inflammation and oxidative stress in health rodents

Abstract

PURPOSE:To investigate the effects of classical acupuncture (Ac) and electroacupuncture (EAc) on estradiol-induced inflammation and oxidative stress in health rodents. METHODS:Twenty-four eight-week old female rats were treated with estradiol valerate (EV) 4.0 mg i.m. single dose and randomly assigned to four groups (n=6): G1(control), G2 (Ac), G3 (EAc 2 Hz) and G4 (EAc 100 Hz). After 60 days all rats were anesthetized with chloral hydrate 10% (0.1 ml/30 g weight of the animal) and submitted to Ac/EAc for twenty minutes. The procedures were repeated on days three, five, seven and nine of the study. The equivalent of the human right ST-36 (Zusanli) and SP-6 (Sanyinjiao) acupoints were chosen for needling and electrical stimulation. On the 10th day of the experiment, all rats were anesthetized for collection of blood and tissues (ovaries) samples for biochemical analysis and histological examination. RESULTS:Glutathione (GSH) and malonaldehyde (MDA) concentrations increased significantly in all groups (plasma and ovary) while myeloperoxidase (MPO) activity decreased significantly in all groups compared with control group (G1). CONCLUSIONS:Both classical acupuncture and electroacupuncture decrease systemic and local oxidative stress and ovary inflammation in healthy rats exposed to estrogenic stimulation. EAc enhances lipid peroxidation at systemic and local levels in female rats exposed to estrogenic stimulation.

Acupuncture inhibits TXNIP‐associated oxidative stress and inflammation to attenuate cognitive impairment in vascular dementia rats

“…Oxidative stress is the result of a disequilibrium of pro‐oxidant and antioxidant systems, which have been well documented in the pathophysiology of CCH‐induced cognitive dysfunction . Acupuncture has proven to have the ability to suppress oxidative stress in many diseases including VD . Our previous study demonstrated that the major ROS‐producing enzyme, NADPH oxidase, was suppressed by acupuncture in VD rats .…”

“…32 Acupuncture has proven to have the ability to suppress oxidative stress in many diseases including VD. 16,33,34 Our previous study demonstrated that the major ROS-producing enzyme, NADPH oxidase, was suppressed by acupuncture in VD rats. 20 In this study, we found that overproduction of ROS and 8-OHdG and SOD inhibitory activity induced by 2VO were reversed by acupuncture treatment.…”

Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats

“…Santos et al 13 studies showed decrease of MPO activity when different types of stimulation were used (AC, EAc -2 and 100 Hz). Reducing the activity of MPO using Ac has already been demonstrated in work recently published by Da Silva et al 23 , using an experimental model of peritonitis in mice.…”

Integrative Health Services in School Health Clinics

“…Doppler ultrasound has shown that acupuncture increases blood flow in treated areas (Lo, Lin, Wei, & Sun, 2013). Finally, thermal imaging shows that acupuncture can decrease inflammation (Santos et al, 2013;Stux, Berman, & Pomeranz, 2000).…”