Oxidative stress evaluation of ischemia and reperfusion in kidneys under various degrees of hypothermia in rats

Santos, Emanuel Burck dos; Koff, Walter José; Filho, Tomáz de Jesus Maria Grezzana; Rossi, Samanta Daiana de; Treis, Lisiane; Bona, Silvia Regina; Pêgas, Karla Laís; Katz, Betina; Meyer, Fabíola Schons; Marroni, Norma Possa; Corso, Carlos Otávio

doi:10.1590/s0102-86502013000800003

Cited by 23 publications

(28 citation statements)

References 17 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, there was no statistically significant difference between animals subjected to kidney ischemia under hypothermic or normothermic conditions. Similar findings were reported previously by our research group in a study of tissue protection by TRH, in which histologic attenuation of renal injuries was not observed 10 .…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Analysis of the effects of topical renal hypothermia on lung tissue after kidney ischemia and reperfusion in rats

et al. 2015

Self Cite

View full text Add to dashboard Cite

PURPOSE:To evaluate whether topical renal hypothermia (TRH) at different levels of temperature has protective effects on lung tissue after renal I/R, through an analysis of organ histology and inflammatory markers in lung tissue. METHODS:Twenty-eight male Wistar rats were randomly allocated across four groups and subjected to renal ischemia at different levels of topical renal temperature: normothermia (no cooling, 37°C), mild hypothermia (26°C), moderate hypothermia (15°C), and deep hypothermia (4°C). To induce I/R, the vessels supplying the left kidney of each animal were clamped for 40 minutes, followed by reperfusion. After four hours, another procedure was performed to harvest the tissues of interest. TNF-α, IL-1β and myeloperoxidase activity were measured in lung tissue. Histological analysis was performed in hematoxylin and eosin-stained lung specimens. RESULTS:Induction of renal I/R under deep topical hypothermia resulted in a significant decrease in lung concentrations of TNF-α compared with normothermic I/R (p<0.05). A trend toward significant correlation was found between lung IL-1β concentration and intensity of hypothermia p=0.055). No difference was found in myeloperoxidase activity or histologic injury between groups. CONCLUSION:Topical renal hypothermia reduces activation of the inflammatory cascade in the lung parenchyma. However, tissueprotective effects were not observed.

show abstract

Section: Discussionsupporting

confidence: 92%

“…Detailed descriptions of the surgical technique, I/R model, and method of renal cooling employed are available in a previous publication by our research group 10 . Briefly, procedures were performed under general anesthesia achieved by intraperitoneal administration of ketamine (75 mg/kg) and xylazine (10 mg/kg).…”

Section: Anesthesia and Surgical Protocolmentioning

confidence: 99%

Analysis of the effects of topical renal hypothermia on lung tissue after kidney ischemia and reperfusion in rats

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…The authors observed that reperfusion accelerated the development of myocardial necrosis, and the degree of myocardial necrosis following I/R for 30-60 min was similar to that observed 24 h after coronary occlusion. To date, various studies have shown that I/R injury is associated with calcium overload (6)(7)(8), the production of free radicals (9,10) and mitochondrial alterations (10,11). Cytosolic calcium overload is now well known as an essential pathophysiological mechanism which is involved in reperfusion injury, although the source and origin of the calcium remains to be determined (12).…”

Section: Introductionmentioning

confidence: 99%

Danshensu protects against ischemia/reperfusion injury and inhibits the apoptosis of H9c2 cells by reducing the calcium overload through the p-JNK-NF-κB-TRPC6 pathway

Meng

Shi

et al. 2015

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Abstract. Ischemia-reperfusion (I/R) plays an important role in myocardial injury. In the present study, we aimed to examine the protective effects of Danshensu (DSS) against I/R injury and to elucidate the underlying mechanisms. For this purpose, H9c2 cells were cultured in hypoxic solution in a hypoxic incubator for 2 h, and then cultured in a high oxygen incubator for various periods of time and pre-treated with or without DSS, ammonium pyrrolidine dithiocarbamate (PDTC) or SP600125 [a c-Jun N-terminal kinase (JNK) inhibitor]. Cell apoptosis and cytosolic free Ca 2+ ([Ca 2+ ] i ) levels were analyzed by flow cytometry. The protein expression levels of JNK, phosphorylated (p-)JNK, nuclear factor-κB (NF-κB) and transient receptor potential cation channel, subfamily C, member 6 (TRPC6) were measured by western blot analysis. The mRNA expression levels of JNK were measured by RT-qPCR. The results revealed that TRPC6 protein expression, the cell apoptotic rate and the [Ca 2+ ] i levels increased in a timedependent manner in the H9c2 cells following the induction of I/R injury. The apoptotic rate and TRPC6 protein expression decreased when the cells were treated with DSS prior to the induction of I/R injury. The knockdown of JNK expression by siRNA decreased the p-JNK and TRPC6 protein expression levels in the H9c2 cells subjected to I/R injury. The protein expression levels of p-JNK and NF-κB in the nucleus increased significantly when the H9c2 cells were subjected to I/R injury, whereas NF-κB expression in the cytoplasm decreased in a time-dependent manner. However, p-JNK, NF-κB and TRPC6 protein expression, the [Ca 2+ ] i level and cell apoptosis decreased when the H9c2 cells were pre-treated with DSS or SP600125. Therefore, our data suggest that DSS prevents myocardial I/R injury by inhibiting p-JNK activation and NF-κB translocation, which potentially upregulate TRPC6 expression, increase the [Ca 2+ ] i level, and result in the apoptosis of H9c2 cells.

show abstract

“…Its absorbance at 535 nm was determined with the aid of a spectrophotometer 10 . To calculate the MDA concentration, an equation fitted to the absorbance curve generated by known MDA concentrations was used [11][12] .…”

Section: Evaluation Of Oxidative Stressmentioning

confidence: 99%

Pulmonary oxidative stress in diabetic rats exposed to hyperoxia

Paiva

Silva²,

Souza³

et al. 2017

Acta Cir. Bras.

View full text Add to dashboard Cite

Pulmonary oxidative stress in diabetic rats exposed to hyperoxia 1 1-Experimental SurgeryActa Cir Bras. 2017;32(7):503-514 AbstractPurpose: To evaluate the pulmonary oxidative stress in diabetic rats exposed to hyperoxia for 90 minutes. Methods: Forty male Wistar rats were divided into four groups, each one containing 10 animals, according to the oxygen concentration to which they were exposed: 21%, 50%, 75% and 100% (hyperoxia). In each group five animals were randomly induced to diabetes by means of at a dose of 55 mg/kg of streptozotocin (STZ). Results: Seventy two hours after diabetes induction, a significant difference was seen in blood glucose in the experimental groups in comparison with the control. In the experimental groups a significant difference was observed in the concentration of malondialdehyde (MDA) in lung tissue and blood plasma (p<0.05), except the 50% group. In the control group, significant differences in the MDA concentration in plasma and lung tissue were also observed (p<0.05), except the 75% group. The MDA concentration in lung tissue in comparison with the diabetic and non-diabetic groups showed a significant difference in the 21% group; however, no difference was seen in the 75 and 100% groups. Conclusion:In diabetic animals high oxygen concentrations (75 and 100%) do not appear to exert deleterious effects on lipid peroxidation in lung tissue.

show abstract

Oxidative stress evaluation of ischemia and reperfusion in kidneys under various degrees of hypothermia in rats

Cited by 23 publications

References 17 publications

Analysis of the effects of topical renal hypothermia on lung tissue after kidney ischemia and reperfusion in rats

Analysis of the effects of topical renal hypothermia on lung tissue after kidney ischemia and reperfusion in rats

Danshensu protects against ischemia/reperfusion injury and inhibits the apoptosis of H9c2 cells by reducing the calcium overload through the p-JNK-NF-κB-TRPC6 pathway

Pulmonary oxidative stress in diabetic rats exposed to hyperoxia

Contact Info

Product

Resources

About