A new proposal for laparoscopic left colectomy in a rat model

Duraes, Leonardo; Duraes, Eliana; Freitas, Pedro F. S.; Carvalho, Felipe Augusto Neves Oliveira de; Carvalho, Sérgio Andurte; Sousa, João Batista de

doi:10.1590/s0102-86502013000400002

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…9 by measuring the bursting pressure using a Zurich brand Z.10.B manometer on the day of euthanasia. Then, a colon segment containing the wound was resected for histopathological analysis and a blood sample was collected for determination of the serum bilirubin level.…”

Section: (8) 2014 -525mentioning

confidence: 99%

Effect of vitamin K1 supplementation on left colon healing in rats with extrahepatic biliary obstruction

Amaral¹,

Alecrim²,

Duarte³

et al. 2014

Acta Cir. Bras.

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Intellectual and scientific content of the study, protocol design, provision of guidelines for surgical intervention, supervision of all phases of the study, manuscript writing, critical revision. ABSTRACT PURPOSE:To evaluate the effects of vitamin K1 on wound healing in the left colon of rats with experimental biliary obstruction. METHODS:Sixteen male rats, divided into four groups of four animals each (L, M, LK, and MK), underwent colostomy followed by bowel suture in the left colon. Seven days before, animals in the L and LK groups had undergone common bile duct ligation. The animals in groups MK and LK received vitamin K1 supplementation. On day 7 after bowel suture, repeat laparotomy was performed for evaluation of colonic healing by burst pressure measurement and collection of samples for histopathological analysis. Changes in body weight were evaluated in the four groups. RESULTS:Weight loss was lower in animals supplemented with vitamin K. No significant differences were observed in burst pressure among the four groups (p>0.05). Histological analysis showed more hemorrhage and congestion in the biliary obstruction groups.Supplemented animals exhibited increased collagen formation and less edema and abscess formation. CONCLUSION:Vitamin K supplementation attenuated weight loss and improved colonic wound healing in rats.

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Section: (8) 2014 -525mentioning

confidence: 99%

Effect of vitamin K1 supplementation on left colon healing in rats with extrahepatic biliary obstruction

Amaral¹,

Alecrim²,

Duarte³

et al. 2014

Acta Cir. Bras.

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“…In the field of gastrointestinal research, the rat colon is widely used to study procedures that require total or partial colectomy including transplantation (Galvão et al., 2006; Zhou et al., 2006), laparoscopic resection (Durães et al., 2013), ulcerative colitis (Schieffer et al., 2017) and anastomosis healing (Akyuz et al., 2018; Boersema et al., 2016; Ghiselli et al., 2020). As these procedures may induce ischaemia and leakage of the anastomosis (Akyuz et al., 2018; Boersema et al., 2016), detailed knowledge of the arterial anatomy is important for accurate and reproducible surgery and to avoid complications.…”

Section: Introductionmentioning

confidence: 99%

Arterial supply to the rat colon

Kigata

Shibata

Kobayashi

2021

Anat Histol Embryol

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Summary Rats are often used as animal models in studies such as on intestinal transplantation and anastomosis healing, which require colectomy. Although detailed information regarding arterial supply is important to establish accurate and reproducible experimental procedures, this has not been studied in the rat colon. Therefore, we analysed the detailed arterial distribution pattern and its individual variations in the colon of 34 rats. The rat colon received colic branches of the ileocolic artery, and the right, middle and left colic arteries. The single left colic artery constantly arose from the caudal mesenteric artery and was distributed to the descending colon, whereas the others showed variations in number and distribution. The ileocolic artery gave rise to one (12%) or two (88%) colic branches supplying the proximal ascending colon, and these branches formed rich, mesh‐like anastomoses along the initial portion of the ascending colon. One (74%) or two (26%) right colic arteries originated from the cranial mesenteric artery and supplied the ascending colon and right colic flexure. Moreover, one (38%), two (56%) or three (6%) middle colic arteries emerged from the cranial mesenteric artery and were distributed to the transverse colon, left colic flexure and proximal descending colon. In total, we categorized the individual variations in arterial branching and anastomosis into 11 patterns. Arterial supply to the rat colon showed a specific pattern and frequent individual variations. These findings thus provide essential information for establishing reproducible models of rat colic surgery.

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International consensus statement regarding the use of animal models for research on anastomoses in the lower gastrointestinal tract

Bosmans

Moossdorff

Beek

et al. 2016

Int J Colorectal Dis

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PurposeThis project aimed to reach consensus on the most appropriate animal models and outcome measures in research on anastomoses in the lower gastrointestinal tract (GIT). The physiology of anastomotic healing remains an important research topic in gastrointestinal surgery. Recent results from experimental studies are limited with regard to comparability and clinical translation.MethodsPubMed and EMBASE were searched for experimental studies investigating anastomotic healing in the lower GIT published between January 1, 2000 and December 31, 2014 to assess currently used models. All corresponding authors were invited for a Delphi-based analysis that consisted of two online survey rounds followed by a final online recommendation survey to reach consensus on the discussed topics.ResultsTwo hundred seventy-seven original articles were retrieved and 167 articles were included in the systematic review. Mice, rats, rabbits, pigs, and dogs are currently being used as animal models, with a large variety in surgical techniques and outcome measures. Forty-four corresponding authors participated in the Delphi analysis. In the first two rounds, 39/44 and 35/39 participants completed the survey. In the final meeting, 35 experts reached consensus on 76/122 items in six categories. Mouse, rat, and pig are considered appropriate animal models; rabbit and dog should be abandoned in research regarding bowel anastomoses. ARRIVE guidelines should be followed more strictly.ConclusionsConsensus was reached on several recommendations for the use of animal models and outcome measurements in research on anastomoses of the lower GIT. Future research should take these suggestions into account to facilitate comparison and clinical translation of results.Electronic supplementary materialThe online version of this article (doi:10.1007/s00384-016-2550-5) contains supplementary material, which is available to authorized users.

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A new proposal for laparoscopic left colectomy in a rat model

Cited by 4 publications

References 8 publications

Effect of vitamin K1 supplementation on left colon healing in rats with extrahepatic biliary obstruction

Effect of vitamin K1 supplementation on left colon healing in rats with extrahepatic biliary obstruction

Arterial supply to the rat colon

International consensus statement regarding the use of animal models for research on anastomoses in the lower gastrointestinal tract

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