Peroneal nerve reconstruction by using glycerol-preserved veins: histological and functional assessment in rats

Cunha, Armando dos Santos; Costa, Márcio Paulino; Silva, Ciro Ferreira da

doi:10.1590/s0102-86502013000200002

Cited by 3 publications

(4 citation statements)

References 18 publications

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“…Alongside the development of synthetic tubes to bridge nerve gaps, a search is currently underway for novel nerve guidance conduits. Blood vessels have been widely used as biologic tubes [ 52 , 86 ]. Importantly, even in the past [ 74 ], successful outcomes were achieved with blood vessels as artificial nerve guides.…”

Section: Tissue-engineered Conduits For Peripheral Nerve Regenerationmentioning

confidence: 99%

“…For example, sciatic nerve transaction leads to gastrocnemius atrophy. The extent of nerve repair was evaluated by weighing the innervated muscle, a histological analysis of muscle fibers, and immunohistochemical staining of pre-and postsynaptic terminals at nerve muscle synapses [ 34 , 54 , 65 , 86 , 96 , 99 ].…”

Section: Evaluation Of the Degree Of Nerve Regeneration After Treatmementioning

confidence: 99%

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Injured Nerve Regeneration using Cell-Based Therapies: Current Challenges

2015

Acta Naturae

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This paper reviews the recent research progress in the past several years on promoting peripheral nerve recovery using stem and progenitory cells. The emphasis is placed on studies aimed at assessing various stem cells capable of expressing neurotrophic and growth factors and surviving after implantation in the nerve or a conduit. Approaches to improving nerve conduit design are summarized. The contribution of stem cells to axonal regeneration and neural repair is discussed. The side effects associated with cell-based treatment are highlighted. From the studies reviewed, it is concluded that the fate of transplanted stem cells needs further elucidation in a microenvironment-dependent manner.

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Section: Tissue-engineered Conduits For Peripheral Nerve Regenerationmentioning

confidence: 99%

Section: Evaluation Of the Degree Of Nerve Regeneration After Treatmementioning

confidence: 99%

Injured Nerve Regeneration using Cell-Based Therapies: Current Challenges

2015

Acta Naturae

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“…Several replacements for nerve grafts have been employed such as vessels 5 All study animals were anesthetized with intraperitoneal injection of sodium pentobarbital (5 mg/kg) before surgical procedure.…”

Section: Studies On Extensive Losses Of Neural Tissue and Theirmentioning

confidence: 99%

Combined polyglycolic acid tube and autografting versus autografting or polyglycolic acid tube alone. A comparative study of peripheral nerve regeneration in rats

Costa

Teixeira²,

Longo

et al. 2015

Acta Cir. Bras.

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PURPOSE:To compare sciatic nerve regeneration in rats using three different techniques of repair. METHODS:Fifteen isogonics rats were divided into three groups according to the method used to repair a 5-mm long defect created in the sciatic nerve: autogenous graft (Group A), polyglycolic acid tube (PGAt) (Group B), and of the association of PGAt with the graft (Group C). Histological analysis, regenerated myelinated axon number count and functional analysis were used to compare after six weeks. RESULTS:There was no difference in fiber diameter and degree of myelinization presented by Groups A, B and C. Group B presented the lowest number of regenerated axons. The groups did not display any significant functional difference after walking track analysis (p<0.05). CONCLUSION:No differences between the three groups in terms of functional recovery, although there were histological differences among them.

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“…Glycerol-preserved veins do not show signs of anatomical demise, which enables their microsurgical use, and renders them less immunogenic 6 , enhance the microenvironment 7 , 8 .…”

Section: Introductionmentioning

confidence: 99%

Comparison of three different strategies to treat sciatic nerve regeneration: an experimental study

et al. 2022

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Purpose: To compare the effect of vein conduit filled with adipose tissue stem cells (ASC) on peripheral nerve injury regeneration. Methods: We analyzed 30 male Wistar rats surgically submitted to a 5-mm gap on the sciatic nerve. Then, the animals were divided into three groups: nerve autografting (AG, n=10), autogenous inverted glycerol-conserved vein (VG, n=10), and autogenous inverted glycerol-conserved vein + ASC (VASCG, n=10). The study endpoints were neuromotor functional analysis, gastrocnemius muscle weight, and sciatic nerve graft histomorphometry analysis. In the histologic analysis, we added a control group (naïve nerve). Results: Regarding functional analysis (Walking tract- score), the findings at week 3 showed a difference between the AG and the VG (-96.6 vs . -59.6, p=0.01, respectively) and between the VG and the inverted vein + VASCG (-59.9 vs . -88.92, p=0.02). At week 12, this study showed a difference between the AG and the VG (-64.8 vs . -47.3, p=0.004, respectively), and also a difference between the VG and the VASCG (-47.3 vs . -57.4, p=0.02, respectively). There was no difference in the histomorphometry analysis (nerve diameter, Schwann cells counting). The gastrocnemius muscles on the intervention side were more atrophic when compared to the gastrocnemius muscles on the control side. Conclusions: Our results suggested better functional recovery in the inverted vein group when compared to control group, and inverted vein + ASC group.

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Peroneal nerve reconstruction by using glycerol-preserved veins: histological and functional assessment in rats

Abstract: Glycerol-preserved autogenous or allogeneic veins showed similar results to autograft results. The autogenous vein (without preservation in glycerol) presented histological and functional outcomes statistically lower than other groups.

Cited by 3 publications

References 18 publications

Injured Nerve Regeneration using Cell-Based Therapies: Current Challenges

Injured Nerve Regeneration using Cell-Based Therapies: Current Challenges

Combined polyglycolic acid tube and autografting versus autografting or polyglycolic acid tube alone. A comparative study of peripheral nerve regeneration in rats

Comparison of three different strategies to treat sciatic nerve regeneration: an experimental study

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