Histological aspects of autologous transplantation of different fragments of the spleen in rats

Abstract

PURPOSE:To evaluate macro and microscopically the evolution of autotransplants of fragments of spleen different fragments in the greater omentum, after eight weeks of observation. METHODS:Twenty rats Wistar were used, males and adults, submitted to total splenectomy and divided in two groups. The group Iten animals with implant of spleen fragment (25% weight of spleen) in the omentum; and group II -ten animals with implant of spleen fragment (30% weight of spleen) in the omentum. It was analyzed macro and microscopically the evolution of the implant. RESULTS:It was observed adherences to the adjacent tissues and vascularization in all of the fragments transplanted. The group I and II presented white pulp with follicular formations and lymphoid tissue preserved, and the red pulp in cordon aspect. The group II presented white pulp more disorganized and red pulp hemorrhagic. The active macrophages were observed in the group I and II. CONCLUSION:The splenic autotransplantation of the group I showed better regeneration.Key words: Transplantation, Autologous. Spleen. Histology. Rats. RESUMO OBJETIVO:Avaliar macro e microscopicamente a evolução do autotransplante de diferentes fragmentos de baço no omento maior, após oito semanas de observação. MÉTODOS:Foram utilizados 20 ratos Wistar, machos e adultos, submetidos a esplenectomia total e distribuídos em dois grupos. O grupo I -dez animais com implante de fragmento com 25% do peso do baço no omento e o grupo II -dez animais com implante de fragmento com 30% do peso do baço no omento. Foram observados macro e microscopicamente a evolução dos implantes. RESULTADOS:Foi observada no fragmento transplantado aderência aos tecidos adjacentes e vascularização preservada. Os grupos I e II apresentaram polpa branca e vascularização preservada, polpa branca com formação folicular e tecido linfóide preservado, e a polpa vermelha com aspecto cordonal. O grupo II apresentou polpa branca mais desorganizada e polpa vermelha hemorrágica. Os macrófagos ativos foram observados nos grupos I e II. CONCLUSÃO:O autotransplante esplênico do grupo I mostrou melhor regeneração.Descritores: Transplante Autólogo. Baço. Histologia. Ratos. Histological aspects of autologous transplantation of different fragments of the spleen in rats

Assessing the spleen autotransplant effect, which common test is the choice?

“…[1,[25][26][27] Although the effectiveness of spleen autotransplantation on different parts of the body, such as the omentum, peritoneal cavity, retroperitoneum, intraportal, abdominal muscle, armpits, and liver, has been proven, the greater omentum has been favored as the appropriate location for autotransplantation. [1,2,6,21,25,28] There are some advantages and disadvantages of selecting the omentum. The advantages include suitable compliance, free space for implanted spleen slices to grow, and efficient revascularization.…”

“…The disadvantages attributed to the selection of this location are intrabdominal implant migration, increasing the rate of intrabdominal adhesions, and abscesses formation. [1,2,4,8,21] The next step after spleen autotransplantation is evaluating the effectiveness and success of the operation. Because blood filtration is an important function of the spleen, determining the presence of HJ bodies is recognized as a method for evaluating the viability of the implanted spleen.…”

“…[1,2] Historically, total splenectomy was the only treatment option for splenic injuries. For a long time, the general belief was that living without the spleen does not cause any significant consequences.…”

“…[1,3,6,7] In order to prevent hematological and immunological disorders, 10% and 25% of the splenic tissue should be preserved, respectively. [2,8] Smaller fragments are insufficient to prevent encapsulated bacterial infections. [9,10] …”

Preliminary study of coconut water for graft tissues preservation in transplantation

“…We then withdrew the abdominal suture, opening the cavity. The four splenic fragments were sutured side by side on the greater omentum, with 4-0 nylon 5 . All procedures were performed under sterile conditions 6 .…”