The effects of anesthetic regimen in 90% hepatectomy in rats

Kieling, Carlos Oscar; Backes, Ariane Nádia; Maurer, Rafael Lucyk; Cruz, Carolina Uribe; Osvaldt, Alessandro Bersch; Silveira, Themis Reverbel da; Matte, Úrsula da Silveira

doi:10.1590/s0102-86502012001000006

Cited by 7 publications

(6 citation statements)

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“…Indeed the survival rate after 90% PH is quite variable. It depends on many factors, including the surgeon's experience, the use of glucose, and the type of anesthesia [ 25 ]. In fact, some authors report 100% survival in one week [ 30 ] whereas others have 0% survival after 2 days [ 31 ], both using glucose supplementation as in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…In brief, the left lateral (30%), left median (40%), and right superior lobes (20%) were removed, leaving only the caudate lobes. Hepatectomy was carried out under isoflurane (Forane, Abbott SA, Argentina) anesthesia [ 25 ]. Immediately after 90% PH and before complete suture, microcapsules (containing 3 × 10 7 WBM cells [ 26 ] or empty) were placed into the peritoneal cavity and glucose was supplemented i.p.…”

Section: Methodsmentioning

confidence: 99%

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Encapsulated Whole Bone Marrow Cells Improve Survival in Wistar Rats after 90% Partial Hepatectomy

Cruz

Kieling

López

et al. 2016

Stem Cells International

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Background and Aims. The use of bone marrow cells has been suggested as an alternative treatment for acute liver failure. In this study, we investigate the effect of encapsulated whole bone marrow cells in a liver failure model. Methods. Encapsulated cells or empty capsules were implanted in rats submitted to 90% partial hepatectomy. The survival rate was assessed. Another group was euthanized at 6, 12, 24, 48, and 72 hours after hepatectomy to study expression of cytokines and growth factors. Results. Whole bone marrow group showed a higher than 10 days survival rate compared to empty capsules group. Gene expression related to early phase of liver regeneration at 6 hours after hepatectomy was decreased in encapsulated cells group, whereas genes related to regeneration were increased at 12, 24, and 48 hours. Whole bone marrow group showed lower regeneration rate at 72 hours and higher expression and activity of caspase 3. In contrast, lysosomal-β-glucuronidase activity was elevated in empty capsules group. Conclusions. The results show that encapsulated whole bone marrow cells reduce the expression of genes involved in liver regeneration and increase those responsible for ending hepatocyte division. In addition, these cells favor apoptotic cell death and decrease necrosis, thus increasing survival.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Encapsulated Whole Bone Marrow Cells Improve Survival in Wistar Rats after 90% Partial Hepatectomy

Cruz

Kieling

López

et al. 2016

Stem Cells International

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“…Hepatectomy was carried out under isoflurane (Forane®, Abbott SA, Argentina) anesthesia [13]. An abdominal midline incision was made to expose the liver.…”

Section: Methodsmentioning

confidence: 99%

Paracrine Effects of Bone Marrow Mononuclear Cells in Survival and Cytokine Expression after 90% Partial Hepatectomy

Kieling

Cruz

López

et al. 2017

Stem Cells International

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Acute liver failure is a complex and fatal disease. Cell-based therapies are a promising alternative therapeutic approach for liver failure due to relatively simple technique and lower cost. The use of semipermeable microcapsules has become an interesting tool for evaluating paracrine effects in vivo. In this study, we aimed to assess the paracrine effects of bone marrow mononuclear cells (BMMC) encapsulated in sodium alginate to treat acute liver failure in an animal model of 90% partial hepatectomy (90% PH). Encapsulated BMMC were able to increase 10-day survival without enhancing liver regeneration markers. Gene expression of Il-6 and Il-10 in the remnant liver was markedly reduced at 6 h after 90% PH in animals receiving encapsulated BMMC compared to controls. This difference, however, was neither reflected by changes in the number of CD68+ cells nor by serum levels of IL6. On the other hand, treated animals presented increased caspase activity and gene expression in the liver. Taken together, these results suggest that BMMC regulate immune response and promote apoptosis in the liver after 90% PH by paracrine factors. These changes ultimately may be related to the higher survival observed in treated animals, suggesting that BMMC may be a promising alternative to treat acute liver failure.

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“…In brief, the left lateral (30%), left median (40%) and the right superior lobes (20%) were removed, leaving only the caudate lobe. Hepatectomy was carried out under isoflurane (Forane ® , Abbott SA, Buenos Aires, Argentina) anaesthesia . Immediately after 90% PH, and before complete laparotomy closure, microcapsules (either empty or containing platelets) were placed into the peritoneal cavity with a 60 ml syringe, and glucose was supplemented i.p.…”

Section: Methodsmentioning

confidence: 99%