Comparison of effects of unfractionated heparin and low molecular weight heparin on skin wound healing of rats

Abstract

PURPOSE:To compare the effects of unfractionated heparin (UH) and a low molecular weight heparin (LMWH) on skin wound healing of rats. METHODS: Forty eight male Sprague-Dawley rats underwent 8mm full thickness dorsal skin wounds and were randomly assigned to three equal groups. In experimental group A, heparin sodium was injected at a concentration of 1000U/kg. In experimental group B, enoxaparin was injected at a concentration of 1mg/kg. Physiologic saline (0.5ml) was administered to the control group. Injections were made subcutaneously, once daily, for seven days. At 7 th and 10 th days tissue samples were taken from all rats. Histologic examination of these tissues was made under light microscope and scored. RESULTS: Histological examination showed a significant difference between the 7 th and 10 th day groups in wound healing. It was observed that wound healing of LMWH injected group is better. This difference is statistically significant at 10 th day. CONCLUSIONS: Daily administration of single doses of unfractionated heparin and a low molecular weight heparin improves wound healing positively. Low molecular weight heparin induces wound healing more than unfractionated heparin.

Heparin-Encapsulated Metered-Dose Topical “Nano-Spray Gel” Liposomal Formulation Ensures Rapid On-Site Management of Frostbite Injury by Inflammatory Cytokines Scavenging

“…These properties of Hp further influence the cell proliferation, collagen restoration, epithelial cell differentiation, and signal transduction pathways in the wound. The function of Hp in the wound-healing process has been established in both cell line and animal studies . In several clinical studies, patients treated with heparin for burns and diabetic foot injury exhibited enhanced capillary circulation that led to reduced wound healing time .…”

“…The function of Hp in the wound-healing process has been established in both cell line and animal studies. 37 In several clinical studies, patients treated with heparin for burns and diabetic foot injury exhibited enhanced capillary circulation that led to reduced wound healing time. 38 The wound healing properties of subcutaneously administered Hp was investigated on burn wounds of rats by Kus and Bienkiewicz, which results in a significant reduction in wound healing time.…”

Effects of apixaban, rivaroxaban, dabigatran and enoxaparin on histopathology and laboratory parameters in Achilles tendon injury: An in vivo study

“…Durmaz et al . [ 17 ] found that enoxaparin was better in the 7 th and 10 th days of wound healing than unfractionated heparin and the control group but did not compare with an oral anticoagulant agent. Although the findings of enoxaparin in the wound healing process differ in the literature, the difficulty of subcutaneous administration compared to oral drug intake restricts the use of this drug.…”

Effect of low molecular weight heparin on autotransplantation efficacy of ovarian tissues in rats

“…The proliferative phase of wound healing is significantly dependent on angiogenesis. Durmaz et al presented the positive effect of enoxaparin on skin wound healing in rats [20]. Norrby et al also presented a positive affect of dalteparin on VEGF-mediated angiogenesis.…”

“…Several authors have presented a negative effect of LMWH on angiogenesis [17][18][19]. On the other hand, the results of more works have shown a positive effect on wound healing and angiogenesis by LMWH application [20,21].…”