Use of 2-octyl cyanoacrylate adhesive in rat liver induced lesion

Abstract

PURPOSE:To evaluate the healing process of rat traumatic liver lesion corrected with the use of 2-octyl cyanoacrylate adhesive, compared to the use of biologically absorbable chromed catgut thread suture. METHODS: Thirty mail adult rats were divided into two groups (15 per group) according to the used method for liver lesion correction as follows: adhesive group (AG), and catgut group (CG); each group being divided into three subsets of five animals (7th, 14th, and 21st day), respectively, according to post-surgery evaluation. All animals were submitted to homogeneous lesion applying synthetic bonding to AG and using chromed catgut suture to CG for lesion correction. Macroscopic and microscopic parameters of healing processes were evaluated. RESULTS: Both groups of animals showed excellent abdominal wall healing, with no evidence of infection, and no abdominal cavity peritonitis or abscess. The presence of adherence was observed in both groups with no statistically significant difference. As to macroscopic evaluation, there was statistically significant difference with respect to specific factors of clinical inflammation (ischemic inflammation and giant celular inflammatory reaction) between animals evaluated on the 10th day (ischemic necrosis and giant celular inflammatory reaction) among animals evaluated on the 14th day (A14 versus C14). CONCLUSION: Applying 2-octyl-cyanoacrylate adhesive for correcting rat liver lesion does not change healing process when compared to the use of chromed catgut stitch. Key words: Wound Healing. Liver. Adhesives. Rats. RESUMO OBJETIVO:Avaliar o processo de cicatrização de lesão induzida em fígado de rato utilizando adesivo de 2-octil cianoacrilato em comparação com a sutura com catgut cromado. MÉTODOS: Utilizou-se 30 ratos adultos, machos, distribuídos em dois grupos de 15 animais denominados de grupo adesivo (GA) e grupo catgut (GC); cada grupo foi dividido em três subgrupos, cada um com cinco animais, de acordo com a data marcada para avaliação pós-operatória (sétimo, décimo quarto e vigésimo primeiro dia). Todos os animais foram submetidos à lesão homogênea, aplicando o adesivo sintético no GA e utilizando sutura com catgut cromado no GC para correção das lesões. Foram avaliados parâmetros de macroscópicos e microscópicos do processo de cicatrização. RESULTADOS: Em ambos os grupos os animais evoluíram com boa cicatrização da parede abdominal, sem evidência clínica de infecção, sem abscessos ou peritonite na cavidade abdominal; a presença de aderências foi observada em ambos os grupos sem diferenças estatisticamente significantes; quanto à avaliação microscópica só houve diferença estatisticamente significante quanto aos parâmetros específicos de inflamação crônica (necrose isquêmica e reação gigantocelular) entre os animais avaliados no décimo quarto dia (A14 vs. C14). CONCLUSÃO: O emprego de adesivo de 2-octil cianoacrilato na correção de lesão em fígado de rato não altera o processo de cicatrização quando comparado ao uso de sutura com fio de categute cromado. Des...

Behaviour of the Biological Adhesives TachoSil®, GelitaSpon®, and a New Elastic Cyanoacrylate (Adhflex®) in Experimental Renal Trauma and Wound Healing

“…A previous study [22] used similar timelines, but others used only a single time point [14,23]. In this case, the study design did not allow wound healing to be explored.…”

“…In comparison, Dos Santos et al [22] compared CyA to conventional suture in traumatic liver injuries in rats. No differences in inflammatory reaction were found between the two treatments, leading the authors to conclude that the adhesive only had a higher number of adhesions than conventional suture 1 week after surgery.…”

Tracking Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesives Biomaterials. Matrix Metalloproteinase Evaluation

“…[10][11][12][13] Consequently, the sampling times used here were defined in line with the inflammatory, proliferative and maturation stages of injury healing. [21] Most MMPs operate simultaneously, at times even sharing substrates, with the activity of one MMP often causing others to become active. It is for that reason that comparisons were made here between groups of MMPs arranged into defined subfamilies.…”

The effect of adhesives on inflammatory immune‐markers during renal injury healing

“…This process prompts inflammatory cells to migrate from the endothelial cells to the injured area. Specific recruitment of these cells is mediated by proinflammatory cytokines and the interaction between adhesion molecules and the cells responsible for defending the organism in these processes …”