Protective effect of N-acetylcysteine on kidney as a remote organ after skeletal muscle ischemia-reperfusion

Takhtfooladi, Mohammad Ashrafzadeh; Jahanshahi, Amirali; Jahanshahi, Gholamreza; Sotoudeh, Ahmad; Takhtfooladi, Hamed Ashrafzadeh; Khansari, Mohammadreza

doi:10.1590/s0102-86502012000900004

Cited by 21 publications

(11 citation statements)

References 31 publications

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“…A secondary damage might then occur later, when kidneys mitochondrial defences are overloaded by a higher amount of oxidative stress and inflammatory factors. Accordingly, we did not observe histological change in the kidney early after reperfusion, but other authors demonstrated tubular cell necrosis after 24 h of reperfusion following a 2-hour femoral artery occlusion [32]. …”

Section: Discussioncontrasting

confidence: 70%

Remote Effects of Lower Limb Ischemia-Reperfusion: Impaired Lung, Unchanged Liver, and Stimulated Kidney Oxidative Capacities

Mansour

Charles

Kindo

et al. 2014

BioMed Research International

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Remote organ impairments are frequent and increase patient morbidity and mortality after lower limb ischemia-reperfusion (IR). We challenged the hypothesis that lower limb IR might also impair lung, renal, and liver mitochondrial respiration. Two-hour tourniquet-induced ischemia was performed on both hindlimbs, followed by a two-hour reperfusion period in C57BL6 mice. Lungs, liver and kidneys maximal mitochondrial respiration (V max⁡), complexes II, III, and IV activity (V succ), and complex IV activity (V TMPD) were analyzed on isolated mitochondria. Lower limb IR decreased significantly lung V max⁡ (29.4 ± 3.3 versus 24 ± 3.7 μmol O2/min/g dry weight, resp.; P = 0.042) and tended to reduce V succ and V TMPD. IR did not modify liver but increased kidneys mitochondrial respiration (79.5 ± 19.9 versus 108.6 ± 21.4, P = 0.035, and 126 ± 13.4 versus 142.4 ± 10.4 μmol O2/min/g dry weight for V max⁡ and V succ, resp.). Kidneys mitochondrial coupling was increased after IR (6.5 ± 1.3 versus 8.8 ± 1.1, P = 0.008). There were no histological changes in liver and kidneys. Thus, lung mitochondrial dysfunction appears as a new early marker of hindlimb IR injuries in mice. Further studies will be useful to determine whether enhanced kidneys mitochondrial function allows postponing kidney impairment in lower limb IR setting.

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Section: Discussioncontrasting

confidence: 70%

Remote Effects of Lower Limb Ischemia-Reperfusion: Impaired Lung, Unchanged Liver, and Stimulated Kidney Oxidative Capacities

Mansour

Charles

Kindo

et al. 2014

BioMed Research International

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“…Major reasons of acute extremity ischemia are thrombosis and embolism 3 . Additionally ischemia reperfusion (IR) can be caused by numerous events such as trauma, various orthopaedic surgery and freeflap reconstruction 4,5 . A huge number of consecutive events are initiated by free oxygen radicals.…”

Section: Introductionmentioning

confidence: 99%

Quercetin protects rat skeletal muscle from ischemia reperfusion injury

Akdemir

Gülçın

Karagöz

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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In this study, we investigated the potential beneficial effects of quercetin on skeletal muscle ischemia reperfusion injury. Twenty-four Sprague-Dawley type rats were randomly divided into four groups. In the sham group, only gastrocnemius muscle were removed and given no quercetin. In ischemia group, all the femoral artery, vein and collaterals were occluded in the left hindlimb by applying tourniquate under general anaesthesia for three hours but reperfusion was not done. In the Quercetin + Ischemia reperfusion group, quercetin (200 mg kg À1 dose orally) was given during one-week reoperation and later ischemia reperfusion model was done. Finally, gastrocnemius muscle samples were removed to measure biochemical parameters. The biomarkers, MDA levels, SOD, CAT and GPx activities, were evaluated related to skeletal muscle ischemia reperfusion injury. MDA levels reduced and SOD, CAT and GPx activities increased significantly in Quercetin + Ischemia reperfusion group. Results clearly showed that Quercetin have a protective role against oxidative damage induced by ischemia reperfusion in rats.

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“…NAC treatment was successful in ameliorating serum creatinine levels in the BDL animals. NAC has been shown to reduce creatinine levels along with exerting protective effect against renal injury as a remote organ after skeletal muscle ischemia-reperfusion in rats [37]. The levels of MDA and conjugated dienes in liver and kidneys from BDL animals were observed to be significantly higher as compared to those observed in SC animals.…”

Section: Discussionmentioning

confidence: 90%

N-acetyl-l-cysteine Prevents Bile Duct Ligation Induced Renal Injury by Modulating Oxidative Stress

2016

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The aim of the present study was to evaluate the effect of N-acetyl-l-cysteine (NAC) on bile duct ligation (BDL) induced oxidative stress in kidneys. Male Wistar rats were randomly segregated into four groups; sham control (SC), SC + NAC, BDL and BDL + NAC group. Liver damage was induced following BDL and renal injury was assessed by kidney function tests along with lipid peroxidation, nitrite levels, thiols and antioxidant enzymes. Three weeks after BDL, rats developed renal dysfunction in terms of elevated serum creatinine levels. BDL animals exhibited an increase in lipid peroxidation, reduction in thiols and redox ratio in liver and kidney tissue along with altered antioxidant enzymes in kidneys. BDL animals that were orally administered NAC at a daily dose 100 mg/kg for duration of two weeks, showed significant reduction in serum creatinine levels. NAC was effective in lowering lipid peroxidation and was able to restore thiol levels along with GSH/GSSG ratio in both liver and kidneys along with the activity of antioxidant enzymes in the kidneys of BDL animals. The results clearly demonstrate the efficacy of NAC in attenuating oxidative stress in kidneys, suggesting a therapeutic role for NAC in individuals with renal dysfunction following BDL.

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Protective effect of N-acetylcysteine on kidney as a remote organ after skeletal muscle ischemia-reperfusion

Cited by 21 publications

References 31 publications

Remote Effects of Lower Limb Ischemia-Reperfusion: Impaired Lung, Unchanged Liver, and Stimulated Kidney Oxidative Capacities

Remote Effects of Lower Limb Ischemia-Reperfusion: Impaired Lung, Unchanged Liver, and Stimulated Kidney Oxidative Capacities

Quercetin protects rat skeletal muscle from ischemia reperfusion injury

N-acetyl-l-cysteine Prevents Bile Duct Ligation Induced Renal Injury by Modulating Oxidative Stress

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