Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

Dornelas, Conceição Aparecida; Fechine-Jamacaru, Francisco Vagnaldo; Albuquerque, Irineu Lima; Magalhães, Hemerson Iury Ferreira; Dias, Thiago Alves; Faria, M.; Alves, Markênia Kely Santos; Rabenhorst, Sílvia Helena Barem; Almeida, Paulo Roberto Carvalho de; Lemos, Telma L. G.; Castro, Jaelson; Moraes, Maria Elisabete Amaral de; Moraes, Manoel Odoríco de

doi:10.1590/s0102-86502012000800003

Cited by 25 publications

(15 citation statements)

References 22 publications

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“…After the segmentation, the software provided the microvascular density of areas studied. The area density was obtained by dividing the area of microvasculature by the total area analyzed [24].…”

Section: Assessment Of Angiogenesis By Immunohistochemistrymentioning

confidence: 99%

“…For this, digital images of the CAM were standardized in four quadrants (top, bottom, right and left) corresponding to the region around the filter paper disk containing the tested compounds. Images acquired were analysed by the System Quantification of Angiogenesis (SQAN) as described above [24]. The parameters analysed for angiogenesis quantification included area of neovascularization, vascular length and total number of blood vessels.…”

Section: In Vivo Chorioallantoic Membrane (Cam) Assaymentioning

confidence: 99%

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Improvement of in vivo anticancer and antiangiogenic potential of thalidomide derivatives

Costa

Carvalho

et al. 2015

Chemico-Biological Interactions

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The strategy of antiangiogenic drugs is based on inhibiting formation of new blood vessels as alternative to limit cancer progression. In this work, we investigated the antitumor and antiangiogenic potential of eight thalidomide derivatives. Most of the molecules was not cytotoxic but 2a, 2d and 3d revealed weak antiproliferative activity on HL-60, Sarcoma 180 (S180) and normal peripheral blood mononuclear cells. Thalidomide, 2a and 2b were able to inhibit tumor growth (53.5%, 67.9% and 67.4%, respectively) in S180-bearing mice and presented moderate and reversible toxicity on liver, kidneys and spleens. Both analogs (2a and 2b) inhibited cell migration of endothelial (HUVEC) and melanoma cells (MDA/MB-435) at 50μg/mL. Immunohistochemistry labeling assays with CD-31 (PECAM-1) antibody showed microvascular density (MVD) was significantly reduced in thalidomide, 2a and 2b groups (30±4.9, 64.6±1.8 and 46.5±19.5%, respectively) (p<0.05). Neovascularization evaluated by Chorioallantoic Membrane Assay (CAM) with compounds 2a and 2b showed reduction of vessels' number (12. 9±2.3 and 14.8±3.3%), neovascularization area (13.1±1.7 and 14.3±1.7%) and total length of vessels (9.2±1.5 and 9.9±1.9%). On the other hand, thalidomide did not alter vascularization parameters. Consequently, addition of thiosemicarbazone pharmacophore group into the phthalimidic ring improved the in vivo antitumor and antiangiogenic potential of the analogs 2a and 2b.

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Section: Assessment Of Angiogenesis By Immunohistochemistrymentioning

confidence: 99%

Section: In Vivo Chorioallantoic Membrane (Cam) Assaymentioning

confidence: 99%

Improvement of in vivo anticancer and antiangiogenic potential of thalidomide derivatives

Costa

Carvalho

et al. 2015

Chemico-Biological Interactions

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“…This work is a continuation of earlier studies published by Dornelas et al 7,9 whose objective was to evaluate the effect of green propolis and L-lysine on carcinogenesis and angiogenesis in rats subjected to chemical carcinogenesis of the bladder. In the earlier studies, we found that propolis administered to rats inhibited angiogenesis and bladder carcinogenesis in this model at the doses studied and that L-lysine promoted bladder carcinogenesis.…”

Section: Discussionmentioning

confidence: 78%

“…Propolis is a resin produced by bees that has various antineoplasic 2 , antiinflammatory 3 , immunomodulatory 4 , antibacterial 5 and antiangiogenesis properties 6,7 . L-lysine is an essential amino acid and has been utilized in the extraction of propolis since the patented proposal of Nicolov et al 8 up to recent publications 2,3,7,9 . The objective of this work was to study the genotoxicity of green propolis and L-lysine on the bone marrow of rats subjected to chemical carcinogenesis of the bladder.…”

Section: Introductionmentioning

confidence: 99%

Potential chemoprotective effects of green propolis, L-lysine and celecoxib on bone marrow cells and peripheral blood lymphocytes of Wistar rats subjected to bladder chemical carcinogenesis

et al. 2014

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PURPOSE:To evaluate the genotoxicity of propolis and L-lysine, as well as their effects on the possible cellular damage in erythroblasts (bone marrow) and leukocytes (peripheral blood) caused by the carcinogen BBN (n -butyl -n {4 -hydroxybutyl} nitrosamine) in rats subjected to bladder carcinogenesis and treated with green propolis and L-lysine. METHODS:One hundred and twenty five rats were distributed into the following groups: I, IIA, IIB, III, K, L M N, X, XI, XII and XIII. Groups I to X received BBN in drinking water for 14 weeks (wks). Group I was treated with intragastric (ig) propolis at 150 mg/ kg body weight, for 44 wks, beginning 30 days before start of BBN. Groups IIA and III were treated with propolis (150 mg/kg), for 40 wks, subcutaneous (sc) and ig, respectively, beginning simultaneously with BBN. On the 32nd wk, the animals of groups L, M and N were treated ig with L-lysine (300 mg/kg), celecoxib (30 mg/kg) and propolis (300 mg/kg), respectively, up to the 40th wk. The groups that received only BBN (IIB and K) were treated with water, sc and orally, respectively, for 40 wks. Groups XI, XII and XIII received respectively propolis (150 mg/kg), L-lysine (150 mg/kg) and water ig for 40 wks. After 40 wks, the surviving animals were anesthetized and subjected to femoral bone marrow aspiration and blood collection from the aorta, for CA and MNT, respectively, for investigation of genotoxicity.RESULTS: Groups IIB and K, which received only BBN and water, showed the greatest DNA damage in peripheral leukocytes (CA) and largest number of micronuclei in bone marrow erythrocytes (MNT) in relation to all other groups that received BBN and lysine and/ or propolis (p<0.001). CONCLUSIONS:Both propolis and L-lysine are effective in protecting against genotoxicity, as well not being genotoxic themselves toward the cells evaluated, at the doses and times administered and according to the two tests utilized.

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“…This fully automated method allows absence of interference from the observer after the acquisition of the images as recommended by Dornelas 22 .…”

Section: Discussionmentioning

confidence: 99%

Oil mixes omega 9, 6 and 3, enriched with seaweed, promoted reduction of thermal burned modulating NF-kB and Ki-67

Campelo

Brito³

et al. 2015

Acta Cir. Bras.

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PURPOSE:To examine the effects of the oil mixes (ω-9, ω-6 and ω-3) in rats subjected to thermal burn. It was also aimed to assess whether the sources of ω3 would interfere with the effect of such mixes on the thermal injury. METHODS:Thirty-six rats distributed into five groups: burned + water, burned + isolipid mix, burned + oil mix 1 (ALA), burned + oil mix 2 (ALA + EPA + DHA of fish) and burned + oil mix 3 (ALA + DHA from seaweed). The thermal injury was involving total thickness of skin. After the burns animals received the oil mixes for seven days. The lesions were evaluated by immunohistochemistry.RESULTS: Animals receiving mix 3 showed a smaller extension of the thermal injury as compared to those that were supplemented with other oils mixes. Expression of Ki-67 in the receiving Mix 3 increased as compared to all the other groups. Animals supplemented with mix 3 were able to inhibit NF-κB in injured tissue. CONCLUSION:Rats received oil mix in which the source of ω3 (ALA+DHA of seaweed) showed inhibition of NF-κB, increase in cell proliferation, and reduction the extension of thermal lesion.Key words: Burn. Fatty Acids. Seaweed. Docosahexenoic Acid. alpha-Linolenic Acid. Rats.Oil mixes omega 9, 6 and 3, enriched with seaweed, promoted reduction of thermal burned modulating

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Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

Cited by 25 publications

References 22 publications

Improvement of in vivo anticancer and antiangiogenic potential of thalidomide derivatives

Improvement of in vivo anticancer and antiangiogenic potential of thalidomide derivatives

Potential chemoprotective effects of green propolis, L-lysine and celecoxib on bone marrow cells and peripheral blood lymphocytes of Wistar rats subjected to bladder chemical carcinogenesis

Oil mixes omega 9, 6 and 3, enriched with seaweed, promoted reduction of thermal burned modulating NF-kB and Ki-67

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