A study of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) serum levels in rats subjected to fecal peritonitis and treated with intraperitoneal ropivacaine

Abstract

A study of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) serum levels in rats subjected to fecal peritonitis and treated with intraperitoneal ropivacaine 1 Avaliação dos níveis séricos de interleucina 6 (IL-6) e fator de necrose tumoral (TNF-α) em ratos submetidos a peritonite fecal e tratado com ropivacaína intraperitoneal ABSTRACT PURPOSE:The objective of this study was to assess the cytokine serum levels of IL-6 and TNF-α in rats subjected to fecal peritonitis and treated with peritoneal lavage with 0.2% ropivacaine by peritoneal lavage. METHODS:We subjected 16 Wistar rats to laparotomy 6 hours after the induction of fecal peritonitis with autogenous stool and subsequently divided the rats randomly into 4 groups: I-control, no treatment; II-drying of the abdominal cavity; III-lavage of the abdominal cavity with 3 mL of 0.9% normal saline and drying; IV-lavage of the abdominal cavity with 3 mL of 0.2% ropivacaine and drying. Six hours following the laparotomy, the animals underwent cardiac puncture, and 1 mL of blood was collected for cytokine assessment before the animals were euthanized. RESULTS:The lavage with ropivacaine resulted in smaller TNF-α levels compared with those observed in the other treatment groups (p <0.05). Regarding IL-6 , the ropivacaine group showed lower cytokine levels than those observed in groups I and II, but there was no significant difference (p> 0.05) between groups III and IV. CONCLUSION:Peritoneal lavage with 0.2% ropivacaine was shown to reduce plasma levels of IL-6 and TNF-α in the treatment of fecal peritonitis in rats.Key words: Peritonitis. Anesthesia. Sepsis. IL-6. TNF-α. RESUMO:OBJETIVO: O objetivo do presente estudo foi avaliar as dosagens séricas das citocinas Il-6 e TNF-α em ratos submetidos à peritonite fecal e tratados com lavagem peritoneal com ropivacaína a 0,2%. MÉTODOS:Utilizaram-se 16 ratos Wistar, submetidos à laparotomia 6 horas após a indução de peritonite fecal com fezes autógenas, distribuídos aleatoriamente em 4 grupos: I-Controle, nenhum tratamento; II-Enxugamento da cavidade abdominal; III-Lavagem da cavidade abdominal com 3 ml de solução salina 0,9% e enxugamento; IV-Lavagem da cavidade abdominal com 3 ml de ropivacaína a 0,2% e enxugamento. Seis horas após a laparotomia os animais foram submetidos à punção cardíaca com retirada de 1 mL de sangue A study of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) serum levels in rats subjected to fecal peritonitis and treated with intraperitoneal ropivacaineActa Cirúrgica Brasileira -Vol. 27 (7) 2012 -495 para a dosagem das citocinas e, a seguir, eutanasiados. RESULTADOS:A lavagem com ropivacaína apresentou valores de TNF-α menores do que os observados com os outros tratamentos (p<0,05). Em relação aos valores da IL-6, o grupo da ropivacaína apresentou valores menores do que os observados com os grupos I e II, mas não houve diferença estatística (p>0,05) em relação ao grupo III. CONCLUSÃO:A lavagem peritoneal com ropivacaína a 0,2% no tratamento da peritonite fecal em ratos demonstro...

Ropivacaine, Interleukin-6 and Tumor Necrosis Factor Alpha Plasma Levels during Intermittent Epidural and Continuous Wound Infusion of Ropivacaine for Analgesia after Hysterectomy or Myomectomy: An Observational Study

“…Previous studies have reported an inverse relationship between postoperative ropivacaine and IL-6 levels [16][17][18] . Also, TNF-α levels decreased initially between 0 and 8 h and then increased between 8 and 24 h. Previous studies on the effect of ropivacaine concentrations on TNF-α levels also produced conflicting results [17,18] . Interestingly, the dose normalized mean IL-6 levels after SC administration were found to be 2.73 times less at 8 h and 2.21 times less at 24 h than that after EP administration.…”

Levobupivacaine inhibits proliferation and promotes apoptosis of breast cancer cells by suppressing the PI3K/Akt/mTOR signalling pathway

“…Lee et al demonstrated that local anaesthetics potentiate TNF-α mediated apoptosis in HK-2 cells [22]. The cellular modification of treated cells is likely dependent on the duration of exposure and the dose of the local anaesthetic [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36]. In this study, we employed MCF-7 and MDA-MB-231 cells as models and found that different concentrations of levobupivacaine could effectively inhibit breast cancer cell proliferation and promote apoptosis in vitro.…”

Levobupivacaine inhibits proliferation and promotes apoptosis of breast cancer cells through PI3K/Akt/mTOR signalling pathway.

“…Recent growing evidence demonstrates that local anaesthetics have an antitumour effect and may suppress the motility of cellular function and invasiveness more likely via voltage-gated sodium channel inhibition. The cellular modi cation of treated cells is likely dependent on the duration of exposure and the dose of local anaesthetic [23][24][25][26][27][28][29][30][31][32][33][34][35][36][37]. In this study, we used MCF-7 and MDA-MB-231 cells as models and found that levobupivacaine could effectively inhibit breast cancer cell proliferation and promotes apoptosis in vitro.…”