Application of Jatropha curcas L. seed oil (Euphorbiaceae) and microcurrent on the healing of experimental wounds in Wistar rats

Passarini, José Roberto; Gaspi, Fernanda de Gaspari de Oliveira; Neves, Lia Mara Grosso; Esquisatto, Marcelo Augusto Marretto; Santos, Gláucia Maria Tech dos; Mendonça, Fernanda Aparecida Sampaio

doi:10.1590/s0102-86502012000700002

Cited by 11 publications

(11 citation statements)

References 28 publications

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“…Komegae et al [23] reported that ES led to a reduction in inflammation responses. Passarini et al [24], Castro et al [25], and Fernanda et al [26] described an increase in the number of newly formed blood vessels and in the progress of epithelization in wounds experimentally induced in Wistar rats treated with microcurrent. Similar results were noted in this study.…”

Section: Discussionmentioning

confidence: 99%

A novel microcurrent dressing for wound healing in a rat skin defect model

Hao

et al. 2019

Military Med Res

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Background The exogenous application of low-intensity electric stimulation (ES) may mimic a natural endogenous bioelectric current and accelerate the repair process of skin wounds. This study designed a novel microcurrent dressing (MCD) and evaluated its potential effects on wound healing in a rat skin defect model. Methods First, wireless ES was integrated into a medical cotton cushion to fabricate the MCD, and its electrical property was examined by using a universal power meter. Then, animal experiments were conducted to evaluate the MCD’s effect. Forty-five rats were randomized into control (Con) group, Vaseline gauze (VG) group and MCD group. A full-thickness round skin incision 1.5 cm in diameter was made on the back of each animal. Apart from routine disinfection, the Con rats were untreated, whereas the other two groups were treated with VG or MCD. On days 3, 7 and 14 post injury, the wound areas were observed and measured using image analysis software following photography, and the skin samples were harvested from wound tissue. Then, histopathological morphology was observed routinely by hematoxylin and eosin (HE) staining; tumor necrosis factor α (TNF-α) and interleukin (IL)-1β expression were detected by Western blotting. Vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) expression were detected with immunohistochemistry. Results The MCD generated a sf electric potential greater than 0.95 V. Animal experiments showed that the wound-healing rate in the MCD group was significantly increased compared with the Con and VG groups ( P < 0.05 or P < 0.01). Histopathological observation revealed an alleviated inflammatory response, induced vascular proliferation and accelerated epithelization in the MCD group. Moreover, samples from the MCD group expressed reduced TNF-α and IL-1β levels and increased VEGF and EGF levels compared with those of the other two groups ( P < 0.05 or P < 0.01). However, no significant difference was noted between the Con and VG groups at each time point. Conclusions The MCD generates a stable and lasting ES and significantly promotes wound healing by reducing inflammation duration and increasing growth factors expression. Thus, MCD may act as a promising biomaterial device for skin wound healing.

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Section: Discussionmentioning

confidence: 99%

A novel microcurrent dressing for wound healing in a rat skin defect model

Hao

et al. 2019

Military Med Res

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“…Micro-current electric stimulator, model EMSI-4250 (made in Taiwan) was used for the treatment. Intensity was 100 microampere ( 10 ) and the frequency 10 Hz. Clip electrodes were used and microcurrent therapy was applied 3 sessions/week ( 11 ).…”

Section: Methodsmentioning

confidence: 99%

Possible Local Stem Cells Activation by Microcurrent Application in Experimentally Injured Soleus Muscle

Zickri

2014

Int J Stem Cells

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Background:Severe injuries in skeletal muscle result in muscle weakness that delays recovery and contribute to progressive decline in muscle function. Microcurrent therapy (MCT) is a novel treatment method used in soft tissue injury and tissue regeneration therapy. The regenerative capacity of skeletal muscle tissue resides in satellite cells, the quiescent adult stem cells.Aim:The present work aimed at investigating the relation between microcurrent therapy and local stem cells in regeneration of induced skeletal muscle injury in albino rat.Materials and methods:Twenty six adult male albino rats were divided into Sham group, Injury group (I): subjected to soleus muscle injury and subdivided into subgroups I1 & I2 sacrificed 2 and 4 weeks after injury respectively. Microcurrent group (M): subjected to muscle injury and micro-current was applied. The animals were subdivided into subgroups M1 and M2 sacrificed 2 and 4 weeks after injury. Histological, immunohistochemical and morphometric studies were performed.Results:Atypical fibers widely separated by infiltrating cells and strong acidophilic sarcoplasm with focal vacuolations were found in injury group. In M1 subgroup few atypical fibers were found. In M2 subgroup multiple typical fibers were detected. A significant decrease in the mean area of atypical fibers, a significant increase in the mean area% of alpha SMA+ve cells and that of CD34+ve cells were found in microcurrent group compared to injury group. Conclusion: A definite therapeutic effect of the microcurrent was found on induced skeletal muscle injury. This effect was proved to be related to satellite cell activation.

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“…A eletroestimulação também se apresenta eficaz na formação de novos vasos sanguíneos (angiogênese), o que foi observado neste estudo, sendo de suma importância para a cicatrização de modo a nutrir tecido lesionado (MENDONÇA, et al, 2009;PASSARINI et al, 2012;CASTRO et al, 2012;YU;HU;PENG, 2014).…”

Section: H Perforatum Modula a Inflamação E A Eletroestimulação Promove Angiogêneseunclassified

“…Sua aplicação é subsensorial não estando associada à sensação desconfortável como outras correntes elétricas, ou seja, é indolor, além de não apresentar efeitos colaterais, ser de baixo custo e de fácil aplicação (MARTELLI et al, 2016). A utilização da eletroestimulação também tem se mostrado bastante promissora e experimentos com correntes elétricas de baixa intensidade (microcorrente) demonstraram efeitos importantes na cicatrização de diferentes tecidos (MENDONÇA et al, 2009;PASSARINI et al, 2012;MOBINI;LEPPIK;BARKER, 2016;SPADARI et al, 2017;FONSECA et al, 2018). A eletroestimulação induz o fluxo de elétrons na pele e tem sido utilizada para restabelecer a atividade bioelétrica endógena de tecidos danificados.…”

Section: Introductionunclassified

Lesões Incisionais Tratadas Com Hypericum Perforatum L. E Eletroestimulação

Esquisatto

2020

REP

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O presente trabalho estudou efeitos de Hypericum perforatum associado ou não à eletroestimulação na cicatrização da pele de ratos. Trinta e seis animais foram divididos em (n=9): (C) sem tratamento; (MC) tratado com eletroestimulação; (HYP) tratado com H. perforatum; (HYP+MC) tratado com H. perforatum+eletroestimulação. Amostras foram coletadas dos animais eutanasiados no 2º, 6º e 10º dia após a lesão cirúrgica para análise histomorfométrica: quantificação de infiltrado inflamatório, vasos sanguíneos, fibroblastos e porcentagem da área de colágeno. Para análise estatística foi utilizado ANOVA Two-way e pós-teste de Tukey (média±erro padrão). Os resultados mostraram que o infiltrado inflamatório foi menor em HYP e MC+HYP em relação a C e MC no 2º e 6º período experimental; no dia 10 a eletroestimulação promoveu aumento do número dessas células. Em relação aos vasos sanguíneos observou-se que MC apresentou maiores valores no 10º período experimental em relação aos demais grupos. No 2º e 6º dia após a lesão, MC+HYP mostrou maior número de fibroblastos em relação aos demais grupos experimentais, o mesmo ocorrendo no 6º dia em MC e HYP em relação a C. Quanto à porcentagem de área de colágeno observou-se aumento em HYP e MC+HYP no 2º dia em relação a C e MC. Diante disso, pode-se concluir que H. perforatum em formulação homeopática neste modelo experimental diminuiu inflamação, promoveu fibroplasia e colagênese. A eletroestimulação diminuiu inflamação e estimulou angiogênese; em associação ou não ao H. perforatum promoveu fibroplasia. Portanto, essas terapias podem ser indicadas como alternativas no reparo de lesões incisionais.

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Application of Jatropha curcas L. seed oil (Euphorbiaceae) and microcurrent on the healing of experimental wounds in Wistar rats

Cited by 11 publications

References 28 publications

A novel microcurrent dressing for wound healing in a rat skin defect model

A novel microcurrent dressing for wound healing in a rat skin defect model

Possible Local Stem Cells Activation by Microcurrent Application in Experimentally Injured Soleus Muscle

Lesões Incisionais Tratadas Com Hypericum Perforatum L. E Eletroestimulação

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