In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts

Bonatti, Silvilena; Hochman, Bernardo; Tucci-Viegas, Vanina Monique; Furtado, Fabianne; Pinfildi, Carlos Eduardo; Pedro, Ana Carolina; Ferreira, Lydia Masako

doi:10.1590/s0102-86502011000100006

Cited by 11 publications

(9 citation statements)

References 26 publications

(47 reference statements)

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“…Opländer et al14 demonstrated that single irradiation with 410 or 420 nm LED at 5~10 J/cm 2 inhibits the proliferation of dermal fibroblasts. Bonatti et al15 also reported a significant reduction in the number of normal fibroblasts irradiated with 470 nm light at 18 J/cm 2 . According to Seo et al16, red (630 nm, 9.5 J/cm 2 ) and green (530 nm, 9.8 J/cm 2 ) LED-irradiated cells were significantly more proliferated than cells irradiated with blue light (460 nm, 27 J/cm 2 ).…”

Section: Discussionmentioning

confidence: 91%

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Low-Level Light Therapy with 410 nm Light Emitting Diode Suppresses Collagen Synthesis in Human Keloid Fibroblasts: AnIn VitroStudy

et al. 2017

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BackgroundKeloids are characterized by excessive collagen deposition in the dermis, in which transforming growth factor β (TGF-β)/Smad signaling plays an important role. Low-level light therapy (LLLT) is reported as effective in preventing keloids in clinical reports, recently. To date, studies investigating the effect of LLLT on keloid fibroblasts are extremely rare.ObjectiveWe investigated the effect of LLLT with blue (410 nm), red (630 nm), and infrared (830 nm) light on the collagen synthesis in keloid fibroblasts.MethodsKeloid fibroblasts were isolated from keloid-revision surgery samples and irradiated using 410-, 630-, 830-nm light emitting diode twice, with a 24-hour interval at 10 J/cm2. After irradiation, cells were incubated for 24 and 48 hours and real-time quantitative reverse transcription polymerase chain reaction was performed. Western blot analysis was also performed in 48 hours after last irradiation. The genes and proteins of collagen type I, TGF-β1, Smad3, and Smad7 were analyzed.ResultsWe observed no statistically significant change in the viability of keloid fibroblasts after irradiation. Collagen type I was the only gene whose expression significantly decreased after irradiation at 410 nm when compared to the non-irradiated control. Western blot analysis showed that LLLT at 410 nm lowered the protein levels of collagen type I compared to the control.ConclusionLLLT at 410 nm decreased the expression of collagen type I in keloid fibroblasts and might be effective in preventing keloid formation in their initial stage.

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Section: Discussionmentioning

confidence: 91%

“…Bonatti et al15 showed that a single irradiation with 470 nm LED at 6, 12, and 18 J/cm 2 does not induce significant differences in the number of keloid fibroblasts. To our knowledge, no study reports that LLLT affects the molecules investigated here in keloid fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Low-Level Light Therapy with 410 nm Light Emitting Diode Suppresses Collagen Synthesis in Human Keloid Fibroblasts: AnIn VitroStudy

et al. 2017

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“…Moreover, the skin exposure to blue light results in antibacterial [ 38 – 40 ], antimicrobial [ 36 , 41 ], and anti-inflammatory [ 42 ] effects. The side effects of blue-light therapy of neonates [ 43 ], suppression of dendritic cell activation [ 44 ] and effect on human dermal fibroblasts [ 45 , 46 ] were also reported. The toxic effect of blue light on the skin was shown to be related to the generation of nonenzymatic nitric oxide (NO) radicals [ 47 – 49 ].…”

Section: Introductionmentioning

confidence: 99%

Blue-Violet Light Irradiation Dose Dependently Decreases Carotenoids in Human Skin, Which Indicates the Generation of Free Radicals

Vandersee

Beyer

Lademann

et al. 2015

Oxidative Medicine and Cellular Longevity

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In contrast to ultraviolet and infrared irradiation, which are known to facilitate cutaneous photoaging, immunosuppression, or tumour emergence due to formation of free radicals and reactive oxygen species, potentially similar effects of visible light on the human skin are still poorly characterized. Using a blue-violet light irradiation source and aiming to characterize its potential influence on the antioxidant status of the human skin, the cutaneous carotenoid concentration was measured noninvasively in nine healthy volunteers using resonance Raman spectroscopy following irradiation. The dose-dependent significant degradation of carotenoids was measured to be 13.5% and 21.2% directly after irradiation at 50 J/cm² and 100 J/cm² (P < 0.05). The irradiation intensity was 100 mW/cm². This is above natural conditions; the achieved doses, though, are acquirable under natural conditions. The corresponding restoration lasted 2 and 24 hours, respectively. The degradation of cutaneous carotenoids indirectly shows the amount of generated free radicals and especially reactive oxygen species in human skin. In all volunteers the cutaneous carotenoid concentration dropped down in a manner similar to that caused by the infrared or ultraviolet irradiations, leading to the conclusion that also blue-violet light at high doses could represent a comparably adverse factor for human skin.

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“…Therefore, the sequential use of red light PBM in the early phase of wound healing and blue light PBM in the later tissue remodeling phase might help to both quicken wound closure and reduce/prevent subsequent fibrosis or wound contracture. In pathological fibrotic lesions, such as keloid, blue PBM might also tilt the balance toward resolution of fibrosis . One important note is that, through the hydroxyproline assay two days after irradiation, Masson‐Meyers et al suggested that blue light PBM does not reduce collagen synthesis.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have shown that blue light PBM may decrease fibroblast activity in vitro , which provides a valuable perspective for anti‐fibrosis treatment. Over‐accumulation of extracellular matrix (ECM) and fibrosis in solid organs such as the lung, kidney, and liver compromises normal physiological functions .…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis Reveals Anti‐Fibrotic Effects of Blue Light Photobiomodulation on Fibroblasts

et al. 2019

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Background and Objectives This study was aimed at determining the effects of blue light photobiomodulation on primary adult mouse dermal fibroblasts (AMDFs) and the associated signaling pathways. Study Design/Materials and Methods Cultured AMDFs from adult C57BL/6 mice were irradiated by blue light from a light‐emitting diode (wavelength = 463 ± 50 nm; irradiance = 5 mW/cm2; energy density = 4–8 J/cm2). The cells were analyzed using mass spectrometry for proteomics/phosphoproteomics, AlamarBlue assay for mitochondrial activity, time‐lapse video for cell migration, quantitative polymerase chain reaction for gene expression, and immunofluorescence for protein expression. Results Proteomic/phosphoproteomic analysis showed inhibition of extracellular signal‐regulated kinases/mammalian target of rapamycin and casein kinase 2 pathways, cell motility‐related networks, and multiple metabolic processes, including carbon metabolism, biosynthesis of amino acid, glycolysis/gluconeogenesis, and the pentose phosphate pathway. Functional analysis demonstrated inhibition of mitochondrial activities, cell migration, and mitosis. Expression of growth promoting insulin‐like growth factor 1 and fibrosis‐related genes, including transforming growth factor β1 (TGFβ1) and collagen type 1 ɑ2 chain diminished. Protein expression of α‐smooth muscle actin, an important regulator of myofibroblast functions, was also suppressed. Conclusions Low‐level blue light exerted suppressive effects on AMDFs, including suppression of mitochondrial activity, metabolism, cell motility, proliferation, TGFβ1 levels, and collagen I production. Low‐level blue light can be a potential treatment for the prevention and reduction of tissue fibrosis, such as hypertrophic scar and keloids. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

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In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts

Cited by 11 publications

References 26 publications

Low-Level Light Therapy with 410 nm Light Emitting Diode Suppresses Collagen Synthesis in Human Keloid Fibroblasts: AnIn VitroStudy

Low-Level Light Therapy with 410 nm Light Emitting Diode Suppresses Collagen Synthesis in Human Keloid Fibroblasts: AnIn VitroStudy

Blue-Violet Light Irradiation Dose Dependently Decreases Carotenoids in Human Skin, Which Indicates the Generation of Free Radicals

Proteomic Analysis Reveals Anti‐Fibrotic Effects of Blue Light Photobiomodulation on Fibroblasts

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