Histological analysis of low-intensity laser therapy effects in peripheral nerve regeneration in Wistar rats

Abstract

Purpose: Analyze the influence of low-intensity laser therapy in the sciatic nerve regeneration of rats submitted to controlled crush through histological analysis. Methods: Were used 20 Wistar rats, to analyze the influence of low-intensity laser therapy in the sciatic nerve regeneration, where the injury of the type axonotmesis was induced by a haemostatic clamp Crile (2nd level of the rack). The animals were randomly distributed in 2 groups. Control group (CG n = 10) and Laser group (LG n = 10). These were subdivided in 2 subgroups each, according to the euthanasia period: (CG14 -n = 5 and CG21 -n = 5) and (LG14 -n = 5 and LG21 -n = 5). At the end of treatment, the samples were removed and prepared for histological analysis, where were analyzed and quantified the following findings: Schwann cells, myelinic axons with large diameter and neurons. Results: In the groups submitted to low-intensity laser therapy, were observed an increase in the number of all analyzed aspects with significance level. Conclusion: The irradiation with low intensity laser (904nm) influenced positively the regeneration of the sciatic nerve in Wistar rats after being injured by crush (axonotmesis), becoming the nerve recovery more rapid and efficient.

Low-Level Laser Therapy (LLLT) Improves the Repair Process of Peripheral Nerve Injuries: A Mini Review

“…The PBMT can provide a decrease in the migration of mononuclear cells, and consequently edema absorption, besides providing an increase in the cellular respiration and an inference in the process of Schwann cells proliferation, allowing a better myelin production [31,32]. As evidenced in the present study, the PBMT increased the diameter of the nerve fibers, offering an increase in the regeneration of the peripheral nerve, being able to favor the elevation in the metabolism of the neurons [33,34].…”

The new heterologous fibrin sealant in combination with low-level laser therapy (LLLT) in the repair of the buccal branch of the facial nerve

“…This finding suggests that despite the satisfactory results obtained in clinical and experimental research, there is a need to carry out further studies to obtain the confirmation of the effects of laser therapy on the repair of peripheral nerves, as well as the standardization of the applied protocols and evaluation parameters [19,20,22,29,44].…”

Effect of low-level laser therapy (LLLT) on peripheral nerve regeneration using fibrin glue derived from snake venom

“…One of the beneficial effects of using low-level laser therapy is based on that light is capable of reducing the migration of mononuclear cells, leading to a decrease in the swelling area as well as an increase in cellular respiration and induction of proliferation in Schwann cells, and increasing the capacity of myelin production [38,39]. The larger diameter of nerve fibers, found in this study, suggests acceleration of the regenerative process after lowlevel laser therapy, which may promote higher metabolism in neurons and a better ability to produce myelin [40,41].…”