Ingestion of polydextrose increase the iron absorption in rats submitted to partial gastrectomy

Abstract

Purpose: To investigate whether polydextrose stimulates iron absorption in rats submitted to partial gastrectomy and sham operated. Methods: The rats were submitted to partial gastrectomy (Billroth II) or laparotomy (sham-operated control), in groups of 20 and 20 each respectively. The animals were fed with a control diet (AIN-93M) without polydextrose or a diet containing polydextrose (50g/Kg of diet) for eight weeks. They were divided into four subgroups: sham-operated and Billroth II gastrectomy and with or without polydextrose. Two animals died during the experiment. All rats submitted to gastrectomy received B-12 vitamin (intramuscular) each two weeks. The hematocrit and hemoglobin concentration were measured at the start and on day 30 and 56 after the beginning of the experimental period. At the end of the study, the blood was collected for determination of serum iron concentration. Results: The diet with polydextrose reduced the excretion of iron. Apparent iron absorption was higher in the polydextrose fed groups than in the control group. The haematocrit and haemoglobin concentration were lower after Billroth II gastrectomy rats fed the control diet as compared to the polydextrose diet groups. Conclusion: Polydextrose increase iron absorption and prevents postgastrectomy anemia. Key words: Prebiotics. Gastrectomy. Iron. Anemia. Rats. RESUMOObjetivo: Investigar se a polidextrose estimula a absorção de ferro em ratos submetidos à gastrectomia parcial e sham operados. Métodos: Os ratos foram submetidos à gastrectomia parcial (Billroth II) e à laparotomia (controle sham-operados) em grupos de 20 e 20 cada, respectivamente. Os animais foram alimentados com uma dieta controle (AIN-93M), sem polidextrose ou uma dieta contendo polidextrose (50g/kg de dieta) durante oito semanas. Foram divididos em quatro grupos: sham-operados e com gastrectomia BII e com ou sem polidextrose. Dois animais morreram durante o experimento. Todos os ratos com gastrectomia receberam vitamina B-12 (intramuscular) a cada duas semanas. O hematócrito e a hemoglobina foram dosados no início e nos dias 30 e 56 após o início do período experimental. No final do estudo, o sangue foi coletado para determinação da concentração de ferro sérico. Resultados: A dieta com polidextrose reduziu a excreção de ferro e a absorção de ferro aparente foi maior nos grupos alimentados com polidextrose do que no grupo controle. As dosagens de hematócrito e hemoglobina foram menores em ratos com gastrectomia alimentados com a dieta controle em relação aos grupos de dieta com polidextrose. Conclusão: A polidextrose aumenta a absorção do ferro e previne a anemia pós-gastrectomia.

A ingestão de prébioticos previne a malabsorção de ferro e anemia induzidas pela gastrectomia?: Estudo experimental em ratos

“…In our previous studies showed that the polydextrose administration increase absorption the iron 19 and calcium 20 in gastrectomized rats. An in vitro study modeling colonic fermentation, showed that polydextrose administration increases the level of bifidobacteria and the level of short chain organic acids, in particular acetic acid.…”

Polydextrose: Physiological Function, and Effects on Health

“…Iron is normally absorbed in the small intestine with the stomach playing an essential role in improving the biological availability of iron [ 27 , 28 , 29 ]. Also, calcium is normally absorbed in the small intestine [ 30 ].…”

Gut Function-Enhancing Properties and Metabolic Effects of Dietary Indigestible Sugars in Rodents and Rabbits

“…Ohta and colleagues confirmed that dietary FOS enhanced Mg absorption and the absorption of Ca, Mg and Fe in Mg-deficient rats and Fe-deficient anemic rats respectively [ 112 , 113 ]. As well, dietary supplements of GOS, FOS, DFA III, polydextrose and epilactose were reported to increase Ca and Fe bioavailability and prevent anemia in gastrectomized animal models [ 111 , 114 , 115 , 116 , 117 ].…”