Histologic alterations of rat kidneys perfused with a Euro-Collins diltiazem solution

Abstract

Purpose: Analyse the histologic changes of rat kidneys perfused with isotonic saline solution (ISS), Euro-Collins solution (ECS) and Euro-Collins solution with diltiazem (ECSD). Methods: Thirty-six Wistar rats were used divided equally, as follow: group A (ISS), group B (ECS) and group C (ECSD). Through a catheter placed into the abdominal aorta, a renal perfusion was performed using a solution according to the group to which the animal belonged. After the complete perfusion, bilateral nephrectomy was performed and the organs were preserved under hypothermia for five distinct periods of time. Glomerulus and tubule were evaluated through optical microscopy. Results: Renal perfusion with ECS and ECSD proved effectiveness in the preservation of the organs up to 36 hours and an increase in the percentage of injured glomeruli was noticed only in the period of 48 hours. Conclusions: The results showed that exists an association between the tubular injury and the glomeruli lesion degree; kidneys with a higher degree of tubular damage were related to severe glomerular lesion. Also, the addition of a calcium channel blocker, diltiazem, to the ECS for the renal perfusion does not decrease the percentage of glomerular lesion. Key words: Diltiazem. Perfusion. Kidney. Rats. RESUMOObjetivo: Analisar as alterações histológicas nos rins de ratos perfundidos com solução salina isotônica (ISS), solução Euro-Collins (ECS) e solução Euro-Collins com diltiazem (ECSD). Métodos: Foram divididos, de forma igual, 36 ratos Wistar, como se segue: grupo A (ISS), grupo B (ECS), grupo C (ECSD). Através de um cateter localizado na aorta abdominal, foi realizada a perfusão renal com a solução de acordo com o grupo ao qual o animal pertencia. Após a perfusão total, realizou-se nefrectomia bilateral com a preservação dos órgãos sob hipotermia por cinco períodos distintos de tempo. Glomérulos e túbulos foram avaliados por microscopia óptica. Resultados: Tanto a perfusão renal com ECS quanto a com ECSD provaram sua efetividade na preservação dos órgãos em até 36 horas e aumento da porcentagem de glomérulos injuriados foi notada apenas no período de 48 horas. Conclusões: Os resultados mostraram haver uma correlação entre a injúria tubular e o grau de lesão glomerular; rins com um maior grau de dano tubular foram relacionados com lesão glomerular severa. Além disso, a adição de um bloqueador de canal de cálcio, diltiazem, à ECS para a perfusão renal não diminui a porcentagem de lesão glomerular. Descritores: Diltiazem. Perfusão. Rim. Ratos.

Hematological, Biochemical, Histopathological and 1H-NMR Metabolomics Application in Acute Toxicity Evaluation of Clinacanthus nutans Water Leaf Extract

“…In addition, there were no structural differences observed in the kidneys’ renal cortex ( Figure 2 b). The renal tubules are considered normal if they are coated by a single layer of cuboid cells densely populated with eosinophils and the tubules are optically empty [ 18 ].…”

Perconditioning combined with postconditioning on kidney ischemia and reperfusion

“…On the other hand, glomeruli were considered as damaged whenever they presented a contraction of glomerular tuft with approximation of structures, contents inside the Bowman's capsule or vacuolization of endothelial cells. The degree of glomerular lesion has been characterized as: 0-No damage; 1-light (up to 25% of damaged glomeruli); 2-moderate (26% to 50% of damaged glomeruli); 3-sharp (51% to 75% of damaged glomeruli); severe (above 75% of damaged glomeruli) 25 .…”

“…Tubular damage was graded at: 0: no damage; 1: 0-25% damaged tubules; 2: 25-50% damaged tubules; 3: 50-75% damaged tubules; 4: >75% damaged tubules 25 .…”