Hyperbaric oxygen therapy and mechanical resistence of the colonics anastomosis in rats with peritonitis

Rocha, Antonio Angelo; Leal, Raquel Franco; Ayrizono, Maria de Lourdes Setsuko; Chung, Wu Feng; Coy, Cláudio Saddy Rodrigues; Lee, Huei; Fagundes, João José

doi:10.1590/s0102-86502010000400013

Cited by 9 publications

(6 citation statements)

References 31 publications

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“…Other two studies corroborate our findings of no significant differences in ischemic necrosis and reepithelization 8,16 .…”

Section: ■ Discussionsupporting

confidence: 92%

“…Previous studies had different results with statistically significant increase in HBOT groups in neovascularization 16,27,30 , and in collagen deposition 8 .…”

Section: ■ Discussionmentioning

confidence: 91%

“…HBOT after the procedure or when the lesion is already in place is a well-established tool in clinical practice; the most commonly studied approach to date is postoperative HBOT [4][5][6][7][8] . Benefits of HBOT preceding the intervention have also been reported, in surgery 9 , in radiotherapy 10,11 , and in procedures related to experimentally induced neurological lesions in animal models 5,12 .…”

Section: ■ Introductionmentioning

confidence: 99%

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Effect of preconditioning and postoperative hyperbaric oxygen therapy on colonic anastomosis healing with and without ischemia in rats

Fontoura-Andrade

Pinto²,

Carneiro

et al. 2020

Acta Cir. Bras.

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“…Other two studies corroborate our findings of no significant differences in ischemic necrosis and reepithelization 8,16 .…”

Section: ■ Discussionsupporting

confidence: 92%

“…Previous studies had different results with statistically significant increase in HBOT groups in neovascularization 16,27,30 , and in collagen deposition 8 .…”

Section: ■ Discussionmentioning

confidence: 91%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of preconditioning and postoperative hyperbaric oxygen therapy on colonic anastomosis healing with and without ischemia in rats

Fontoura-Andrade

Pinto²,

Carneiro

et al. 2020

Acta Cir. Bras.

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“…They showed that delay in granulation tissue formation and fibroblast proliferation significantly influenced the therapy groups. Rocha et al 57 showed that hyperbaric oxygen therapy did not alter the mechanical resistance of anastomosis in distal colon of rats in the presence of peritonitis. Murray 33 found that impairment due to an increase in the collagen and protein synthesis in the early postoperative colon surgery after radiation returned to the normal levels on the fourth month.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Hyperbaric Oxygen Therapy on Experimental Colon Anastomosis After Preoperative Chemoradiotherapy

Yıldız¹,

Can²,

Yağcı³

et al. 2013

International Surgery

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The aim of the present study was to investigate the effect of hyperbaric oxygen therapy (HBOT) on colon anastomosis after chemoradiotherapy (CRT). Sixty female WistarAlbino rats were divided into 5 groups and underwent left colon resection and end-toend anastomosis. CRT simulation was performed on 2 sham groups before the anastomosis, and 1 of these groups was administered additional postoperative HBOT. Two groups were administered CRT before the anastomosis, and 1 of them received additional postoperative HBOT. On postoperative day 5, all groups underwent relaparotomy; burst pressure was measured and samples were obtained for histopathologic and biochemical analysis. There was a significant weight loss in the CRT groups and postoperative HBOT had an improving effect. Significantly decreased burst pressure values increased up to the levels of the controls after HBOT. Hydroxyproline levels were elevated in all groups compared to the control group. Hydroxyproline levels decreased with HBOT after CRT. No significant difference was observed between the groups regarding fibrosis formation at the anastomosis site. However, regression was observed in fibrosis in the group receiving HBOT after CRT. Preoperative CRT affected anastomosis and wound healing unfavorably. These unfavorable effects were alleviated by postoperative HBOT. HBOT improved the mechanical and biochemical parameters of colon anastomosis in rats.Key words: Anastomosis -Chemoradiotherapy -Hyperbaric oxygen -Rat -Burst pressure 2 Although adjuvant therapy has been a well-accepted procedure to prevent metastasis and local recurrence after surgical resection, more recently neoadjuvant therapy has gained popularity to enable secondary curative resection with clear surgical margin and to prolong overall and recurrence-free survival.2 It has been reported that local recurrence rate is lower, toxicity is rare, and the anal sphincter is more likely to be preserved in those undergoing preoperative radiation. 1,2Despite the current advances in surgical techniques, anastomotic leakage is a severe complication leading to morbidity and mortality after intestinal surgery.3-6 Chemoradiotherapy (CRT) has the potential to lead to inadequate vascularization in tissues, a well-known risk factor for intestinal anastomosis leak. Impaired oxygenation as a result of hampered vascularization may lead to delay in wound healing, which ultimately impairs anastomotic durability. Studies on factors that enhance wound healing to prevent anastomotic leakage in colon anastomosis have been accelerated in the past decade. In a limited number of studies performed for this purpose, hyperbaric oxygen therapy (HBOT), which is applied by means of intermittent inhalation of 100% oxygen under 1 atmosphere pressure, has been reported to have favorable effects on intestinal anastomotic healing.7-9 However, whether HBOT can reverse potential side effects of CRT on would healing and anastomotic durability is unclear. The aim of the present study was to investigate the effects of HBOT on the bioche...

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“…Rocha et al 13 demonstrated that peritonitis made the total bursting energy values decrease significantly on the fourth day after performing anastomosis in rats' distal colon. De Hing et al 14 evidenced that peritonitis decreased the anastomosis' mechanical resistance on the third post-operatory day, returning to normality on the fifth day.…”

Section: Discussionmentioning

confidence: 99%

Study on adhesion formation and the healing of colon anastomosis in rats with induced peritoneal sepsis

Silva¹,

Oliveira

Brandão

et al. 2011

Acta Cir. Bras.

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PURPOSE:To evaluate the effects of abdominal sepsis on adhesion formation and colon anastomosis healing in rats. METHODS: Forty rats were distributed in two groups containing 20 rats each for left colon anastomosis in the presence (Group S) or absence (Group N) of induced sepsis by cecal ligation and puncture. Each group was divided into subgroups for euthanasia on the third (N3 and S3) or seventh (N7 or S7) post-operative day. The amount of adhesions was evaluated and a segment of the colon was removed for histopathologic analysis, bursting strength assessment, hydroxyproline and the determination of tissue collagen. RESULTS: The subjects which underwent cecal ligation and puncture presented a higher amount of intra-abdominal adherences in both third (p=0,00) and seventh (p=0,00) post-operatory days. Smaller bursting strengths were found in the S3 subgroup, and greater bursting strengths were found in the S7 subgroup. There was no difference in the variations on the concentrations of hydroxyproline, tissue collagen and histopathology. CONCLUSIONS: The peritoneal infection which was developed by cecal ligation and puncture raised the amount of intra-cavitary adhesions. There was a decrease in the amount of colonic anastomosis on the third post-operatory day with a following raise on the seventh without any effects on other healing parameters. Key words: Anastomosis, Surgical. Colon. Sepsis. Tissue Adhesions. Rats. RESUMO OBJETIVO:Avaliar os efeitos da sepse abdominal sobre a formação de aderências e a cicatrização de anastomoses colônicas em ratos. MÉTODOS: 40 ratos distribuídos em dois grupos contendo 20 animais, para anastomose do cólon esquerdo na presença (grupo S) ou ausência (grupo N) de indução de sepse por ligadura e punção do ceco (CLP). Cada grupo foi dividido em subgrupos para eutanásia no terceiro (N3 e S3) ou sétimo (N7 e S7) dia de pós-operatório (DPO). Foi avaliada a quantidade de aderências e removido um segmento colônico contendo a anastomose para análise histopatológica, força de ruptura, hidroxiprolina e conteúdo de colágeno tecidual. RESULTADOS: Os animais submetidos à CLP apresentaram maior quantidade de aderências intra-abdominais tanto no 3° DPO (p=0,00) quanto no 7° DPO (p=0,00). Tiveram menores valores de força de ruptura no 3° DPO (p=0,00), porém maiores valores no 7°D PO (p=0,00). Não houve diferença na variação da concentração de hidroxiprolina, conteúdo de colágeno e histopatologia. CONCLUSÕES: A infecção peritoneal desencadeada por CLP aumentou a quantidade de aderências intra-cavitárias. Houve diminuição da resistência de anastomoses cólicas no 3° DPO, com posterior aumento no 7° DPO, sem efeito sobre os outros parâmetros da cicatrização. Descritores: Anastomose Cirúrgica. Cólon. Sepse. Aderências Teciduais. Ratos. Study on adhesion formation and the healing of colon anastomosis in rats with induced peritoneal sepsis

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Hyperbaric oxygen therapy and mechanical resistence of the colonics anastomosis in rats with peritonitis

Cited by 9 publications

References 31 publications

Effect of preconditioning and postoperative hyperbaric oxygen therapy on colonic anastomosis healing with and without ischemia in rats

Effect of preconditioning and postoperative hyperbaric oxygen therapy on colonic anastomosis healing with and without ischemia in rats

The Effects of Hyperbaric Oxygen Therapy on Experimental Colon Anastomosis After Preoperative Chemoradiotherapy

Study on adhesion formation and the healing of colon anastomosis in rats with induced peritoneal sepsis

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