Porcine peritoneum as source of biocompatible collagen in mice

Abstract

Purpose:To investigate the biocompatibility and biodegradability of a membrane made from porcine peritoneum. Methods: The membrane (5x5 mm) was inserted in the subcutaneous tissue on the back of 15 mice, which were killed after 1, 3 and 9 weeks (ISO 10993-6). The cellular components of the inflammatory response and degradation of the membrane were analyzed in hematoxylin-eosin-stained histological sections. Results: After one week, mononuclear cells were observed inside the membrane. After three weeks, the material was almost completely absorbed. After nine weeks, there was no presence of material and there were signs of tissue remodeling. There was neither a foreign body reaction nor signs of tissue necrosis. Conclusion: The collagen membrane derived from porcine peritoneum is biocompatible and bioabsorbable when implanted in the subcutaneous tissue of mice. Key words: Materials Testing. Biocompatible Materials. Subcutaneous Tissue. Peritoneum. Mice RESUMO Objetivo: Investigar a biocompatibilidade e biodegradabilidade de uma membrana feita de peritônio suíno. Métodos: A membrana (5x5 mm) foi inserida no tecido subcutâneo dorsal de 15 camundongos, que foram mortos após uma, três e nove semanas (ISO 10993-6). Os componentes celulares da resposta inflamatória e a degradação da membrana foram analisados em cortes corados em hematoxilina-eosina. Resultados: Após uma semana, células mononucleares foram observadas dentro da membrana. Após três semanas, o material foi quase completamente absorvido. Após nove semanas, não houve presença do material e houve sinais de remodelação tecidual. Não houve reação de corpo estranho nem sinais de necrose tecidual. Conclusão: A membrana colágena derivada de peritônio suíno é biocompatível e bioabsorvível quando implantada no tecido subcutâneo do camundongo. Descritores: Teste de Materiais. Materiais Biocompatíveis. Tela Subcutânea. Peritônio. Camundongos.

Effect of Extracellular Matrix Membrane on Bone Formation in a Rabbit Tibial Defect Model

“…Collagen is accepted as a safe material and also has a nontoxic degradation product [4–6]. However, its degradation period is uncontrolled, with a minimum period of 2 weeks and maximum of 8 months after implantation.…”

The Condensation of Collagen Leads to an Extended Standing Time and a Decreased Pro-inflammatory Tissue Response to a Newly Developed Pericardium-based Barrier Membrane for Guided Bone Regeneration

“…For example, collagen has a chemotactic effect on fibroblasts, promoting tissue integration (9). Collagen-based barrier membranes have already been extensively investigated in both animal experiments and clinical studies (3,(14)(15)(16)(17)(18)(19). Although the clinical results related to collagen-based membranes are comparable to those of non-resorbable membranes, their application has a lower incidence of spontaneous exposures (20,21).…”

Polysaccharide-Based Membrane Biocompatibility Study of Anacardium occidentale L. and Polyvinyl Alcohol after Subcutaneous Implant in Rats

“…There are few reports of polymeric biomaterials that maintain their integrity for 30 days or longer in murine subcutaneous tissue [37,38], although partial or total degradation occurs within 21 days [39,40] or even in 7 days [41]. In this study, the presence of the CGP/PVA membrane was observed within 60 days of implantation.…”