2010
DOI: 10.1590/s0102-86502010000300011
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Abstract: Purpose: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that displays a rapid evolution. Current treatments have failed to revert clinical symptoms because the mechanisms involved in the death of motoneuron are still unknown. Recent publications have put non-neuronal cells, particularly, astrocyte and microglia, in the scenario of pathophisiology of the disease. Animal models for ALS, particularly transgenic mice expressing the human SOD1 gene with a G93A mutation (hSOD1), are availab… Show more

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Cited by 16 publications
(13 citation statements)
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“…We followed a modified protocol to establish pure microglial cultures from P0 Wistar rat pups [29]. Briefly, the spinal cords were dissected, freed of meninges and mechanically triturated in Dulbecco’s Modified Eagle Medium (DMEM) and propagated in DMEM with 10% FBS (GIBCO-BRL).…”
Section: Methodsmentioning
confidence: 99%
“…We followed a modified protocol to establish pure microglial cultures from P0 Wistar rat pups [29]. Briefly, the spinal cords were dissected, freed of meninges and mechanically triturated in Dulbecco’s Modified Eagle Medium (DMEM) and propagated in DMEM with 10% FBS (GIBCO-BRL).…”
Section: Methodsmentioning
confidence: 99%
“…Transgene SOD1 G93A mice (The Jackson Laboratory, Bar Harbor, ME, USA) were crossbred and the colony was maintained in a specific pathogen-free environment of the animal facility of University of São Paulo Medical School (São Paulo, Brazil) as described previously (Gurney, 1994; Scorisa et al, 2010; Alves et al, 2011). Animals were kept under controlled temperature and humidity conditions with a standardized light–dark cycle (lights on at 7.00 a.m. and off at 7.00 p.m.) and free access to food pellets and tap water.…”
Section: Methodsmentioning
confidence: 99%
“…Animals were kept under controlled temperature and humidity conditions with a standardized light–dark cycle (lights on at 7.00 a.m. and off at 7.00 p.m.) and free access to food pellets and tap water. Mice were genotyped by PCR amplification of DNA extracted from their tails in order to identify the SOD1 mutation (Gurney, 1994; Scorisa et al, 2010; Alves et al, 2011). The study was conducted under protocols approved by the Animal Care and Use of Ethic Committee at University of São Paulo and in accordance to the Guide for the Care and Use of Laboratory Animals adopted by the National Institutes of Health.…”
Section: Methodsmentioning
confidence: 99%
“…For genotyping, the DNA was extracted from all mice tails by using a protocol described previously (Scorisa et al, 2010). Briefly, 500 μl of 1 mg/ml K proteinase buffer (Sigma, USA) (Tris-HCl 20 mM pH 8.0, NaCl 10 mM, EDTA 30 mM pH8.0 and SDS 0.5%) was added to each tail sample.…”
Section: Animalsmentioning
confidence: 99%