2010
DOI: 10.1590/s0102-86502010000200015
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Abstract: Purpose: Standardization of a simple and low cost technique of exhaled breath condensate (EBC) collection to measure nitrite. Methods: Two devices were mounted in polystyrene boxes filled either with crushed ice/salt crystals or dry ice/crushed ice. Blood samples were stored at -70º C for posterior nitrite dosages by chemiluminescence and the Griess reaction. Results: a) The use of crushed ice/dry ice or salt revealed sufficient EBC room air collection, but was not efficient for patients under ventilation supp… Show more

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Cited by 7 publications
(6 citation statements)
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“…In this study, there were no significant correlations between EBC and plasma NO concentrations in the preoperative and postoperative periods of CABG and valve surgeries. This finding is similar to the results reported by Dillon et al [15] and Biban et al [12], who found no correlation between exhaled NO and plasma NO 2 -and NO 3 -levels. This could indicate that exhaled NO, at least in healthy conditions, is more reflective of local NO release than systemic-derived NO.…”
Section: Discussionsupporting
confidence: 92%
“…In this study, there were no significant correlations between EBC and plasma NO concentrations in the preoperative and postoperative periods of CABG and valve surgeries. This finding is similar to the results reported by Dillon et al [15] and Biban et al [12], who found no correlation between exhaled NO and plasma NO 2 -and NO 3 -levels. This could indicate that exhaled NO, at least in healthy conditions, is more reflective of local NO release than systemic-derived NO.…”
Section: Discussionsupporting
confidence: 92%
“…In conclusion EBC can be used to demonstrate inflammation noninvasively in the lungs of those with lung transplants. Markers of nonspecific inflammation such as nitrogen oxides and hydrogen peroxide are inexpensive, simple assays of markers that are reliably detected in EBC compared to cytokine levels, and the clinical applicability of these markers warrants further research [14,24]. Further development is needed to assess the potential of cytokines in the EBC as a diagnostic tool with more sensitive assays or methods of concentrating EBC to ensure that the appropriate analyses may be performed in all subjects.…”
Section: Discussionmentioning
confidence: 99%
“…This is because NO reacts in a nearly diffusion-limited reaction with both oxyhemoglobin (6 to 8x10 7 mol/L/sec) and deoxyhemoglobin (2 to 6x10 7 mol/L/ sec) to form methemoglobin/nitrate and iron nitrosyl hemoglobin, respectively, which yield a half-life of ~1 µs and a diffusion distance of ~0.1 µm [34]. Monitoring nitrite using exhaled breath condensate (EBC) is another alternative [61], but analysis is complicated due to the substantial contribution of nitrite from the oropharyngeal tract during standard collection of EBC [62].…”
Section: Current Methods For Measurement Of No and No 2 -mentioning
confidence: 99%